Research Output

1988

Active calf chymosin secreted from Trichoderma reesei

Harkki, A., Uusitalo, J., Bailey, M., Penttilä, M. & Knowles, J., 1988, Biotieteen päivät. Järvenpää, 17. - 18.5.1988. Abstract. Helsinki, p. II-10

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsScientific

1989

A novel fungal expression sys1em: Secretion of active calf chymosin from the filamentous fungus trichoderma reesei

Harkki, A., Uusitalo, J., Bailey, M., Penttila, M. & Knowles, J. K. C., 1 Jan 1989, In : Bio/Technology. 7, 6, p. 596-603 8 p.

Research output: Contribution to journalArticle

108 Citations (Scopus)
1990

Secretion of active calf chymosin by Trichoderma reesei

Uusitalo, J., Harkki, A., Penttilä, M., Keränen, S., Hirvonen, A. & Knowles, J., 1990, 6th International Symposium Genetics of Industrial Microorganisms (GIM 90). Strasbourg, 12 - 18 Aug. 1990: Abstract. Strasbourg, 171

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsScientific

Trichoderma: A fungal expression system for the production of heterologous proteins

Penttilä, M., Uusitalo, J., Hirvonen, A., Nyyssönen, E., Saloheimo, M., Keränen, S. & Knowles, J., 1990, 5th European Congress Biotechnology, Copenhagen, Denmark, 8 - 13 July 1990: Proceedings. Christiansen, C., Munck, L. & Villadsen, J. (eds.). Copenhagen, p. 200-202

Research output: Chapter in Book/Report/Conference proceedingConference article in proceedingsScientific

1991

Enzyme production by recombinant Trichoderma reesei strains

Uusitalo, J. M., Helena Nevalainen, K. M., Harkki, A. M., Knowles, J. K. C. & Penttilä, M. E., 1 Jan 1991, In : Journal of Biotechnology. 17, 1, p. 35-49 15 p.

Research output: Contribution to journalArticleScientificpeer-review

80 Citations (Scopus)

Protein engineering of chymosin and expression in Trichoderma reesei

Pitts, J. E., Quinn, D., Uusitalo, J. & Penttila, M., 1 Jan 1991, In : Biochemical Society Transactions. 19, 3, p. 663-666 4 p.

Research output: Contribution to journalArticle

9 Citations (Scopus)

Protein engineering of the aspartic proteinase chymosin: Abstract

Nugent, P., Pitts, J., Orprayoon, P., Mantafounis, D., Quinn, D., Uusitalo, J. & Penttilä, M., 1991, Protein Engineering and Design, London, England, 16 - 17 Dec. 1991. London, P13

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsScientific

Protein engineering of the surface loops of chymosin

Orprayoon, P., Pitts, J. E., Nugent, P., Mantafounis, D., Lawler, S. E., Blundell, T. L., Uusitalo, J. & Penttila, M., 2 Oct 1991, In : Biochemical Society Transactions. 19, 3

Research output: Contribution to journalArticle

1 Citation (Scopus)

Secretion of a chymosin-insulin fusion in Trichoderma reesei

Lawler, S. E., Pitts, J. E., Mantafounis, D., Gill, R., Uusitalo, J. & Penttila, M., 2 Oct 1991, In : Biochemical Society Transactions. 19, 3

Research output: Contribution to journalArticle

1992

Protein engineering of loops in chymosin and expression in Trichoderma reesei

Nugent, P., Pitts, J., Orprayoon, P., Blundell, T., Uusitalo, J. & Penttilä, M., 1992, 1st European Conference on Fungal Genetics. Nottingham: University of Nottingham, P2/73

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsScientific

1993

Expression and characterisation of chymosin pH optima mutants produced in Trichoderma reesei

Pitts, J. E., Uusitalo, J. M., Mantafounis, D., Nugent, P. G., Quinn, D. D., Orprayoon, P. & Penttilä, M. E., 1 Jan 1993, In : Journal of Biotechnology. 28, 1, p. 69-83 15 p.

Research output: Contribution to journalArticle

9 Citations (Scopus)

Prarbn engineering of a loop 155-165 in chymosin

Orprayoon, P., Nugent, P., Pitts, J. E., Blundell, T. L., Uusitalo, J. M. & Penttila, M., 1 Jan 1993, In : Protein Engineering, Design and Selection. 6, 1 p.

Research output: Contribution to journalArticle

Production of chymosin mutants in Trichoderma reesei

Pitts, J., Nugent, P., Orprayoon, P., Uusitalo, J. & Penttilä, M., 1993.

Research output: Contribution to conferenceConference AbstractScientific

1995

Protein engineering of surface loops: Preliminary X-ray analysis of the CHY155-165RHI mutant

Dhanaraj, R. R. V., Pitts, J. E., Nugent, P., Orprayoon, P., Cooper, J. B., Blundell, T. L., Uusitalo, J. & Penttila, M., 1 Jan 1995, Aspartic Proteinases: Structure, Function, Biology, and Biomedical Implications. Takahashi, K. (ed.). Boston: Springer, p. 95-99 (Advances in Experimental Medicine and Biology, Vol. 362).

Research output: Chapter in Book/Report/Conference proceedingConference article in proceedingsScientificpeer-review

1996

Kinetic analysis of protein production in the filamentous fungus Trichoderma reesei

Pakula, T., Salonen, K., Uusitalo, J., Saloheimo, M., Verboon, S., Aarts, R. & Penttilä, M., 1996.

Research output: Contribution to conferenceConference articleScientific

Kinetics of protein secretion in Trichoderma reesei

Pakula, T., Salonen, K., Uusitalo, J., Saloheimo, M., Aarts, R. & Penttilä, M., 1996.

Research output: Contribution to conferenceConference articleScientific

Kinetics of Protein Secretion in Trichoderma Reesei

Pakula, T., Salonen, K., Uusitalo, J., Saloheimo, M., Aarts, R. J. & Penttilä, M., 1996, Abstracts from the 3rd ECFG: European Congress on Fungal Genetics. New Prairie Press, (Fungal Genetics Reports; No. 34, Vol. 43).

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsScientific

Open Access
1997

Kinetics of protein secretion in Trichoderma reesei

Pakula, T., Salonen, K., Uusitalo, J., Saloheimo, M., Aarts, R. & Penttilä, M., 1997.

Research output: Contribution to conferenceConference articleScientific

Mutagenesis, biochemical characterization and X-ray structural analysis of point mutants of bovine chymosin

Williams, M. G., Wilsher, J., Nugent, P., Mills, A., Dhanaraj, V., Fabry, M., Sedlácek, J., Uusitalo, J. M., Penttila, M. E., Pitts, J. E. & Blundell, T. L., 1 Dec 1997, In : Protein Engineering. 10, 9, p. 991-997 7 p.

Research output: Contribution to journalArticle

17 Citations (Scopus)
1998

Engineering of the carbohydrate moiety of fungal proteins to a mammalian type

Maras, M., De Bruyn, A., Schraml, J., Herdewijn, P., Piens, K., Claeyssens, M., Uusitalo, J., Penttilä, M., Fiers, W. & Contreras, R., 1998, Carbohydrases from Trichoderma reesei and other microorganisms: structures, biochemistry, genetics and applications. Claeyssens, M., Nerinckx, W. & Piens, K. (eds.). Royal Society of Chemistry RSC, p. 323-326 (Special Publication; No. 219).

Research output: Chapter in Book/Report/Conference proceedingConference article in proceedingsScientific

Kinetic analysis of protein secretion in Trichoderma reesei

Uusitalo, J., Pakula, T., Salonen, K., Parkman, S., Kurkela, M., Huuskonen, A., Aristidou, A., Aarts, R. & Penttilä, M., 1998.

Research output: Contribution to conferenceConference articleScientific

Ultrastructural study indicates that fusion of recombinant calf chymosin to cellobiohydrolase I enhances its passage through the mature cell wall of Trichoderma reesei

Nykänen, M., Uusitalo, J., Mikkonen, A. & Penttilä, M., 1998, p. 111-112.

Research output: Contribution to conferenceConference articleScientific

1999

In vivo synthesis of complex N-glycans by expression of human N-acetylglucosaminyltransferase I in the filamentous fungus Trichoderma reesei

Maras, M., De Bruyn, A., Vervecken, W., Uusitalo, J., Penttilä, M., Busson, R., Herdewijn, P. & Contreras, R., 11 Jun 1999, In : FEBS Letters. 452, 3, p. 365-370 6 p.

Research output: Contribution to journalArticleScientificpeer-review

34 Citations (Scopus)

Kinetics of protein secretion in the filamentous fungus Trichoderma reesei

Pakula, T., Riikonen, M., Uusitalo, J., Saloheimo, M., Salonen, K. & Penttilä, M., 1999.

Research output: Contribution to conferenceConference articleScientific

2000

Kinetics of protein secretiom in the filamentous fungus Trichoderma reesei

Pakula, T., Riikonen, M., Uusitalo, J., Saloheimo, M., Salonen, K. & Penttilä, M., 2000.

Research output: Contribution to conferenceConference articleScientific

Kinetics of protein secretion in the filamentous fungus Trichoderma reesei

Pakula, T., Riikonen, M., Uusitalo, J., Saloheimo, M. & Penttilä, M., 2000.

Research output: Contribution to conferenceConference articleScientific

Monitoring the kinetics of glycoprotein synthesis and secretion in the filamentous fungus Trichoderma reesei: Cellobiohydrolase I (CBHI) as a model protein

Pakula, T. M., Uusitalo, J., Saloheimo, M., Salonen, K., Aarts, R. J. & Penttilä, M., 1 Jan 2000, In : Microbiology. 146, 1, p. 223-232 10 p.

Research output: Contribution to journalArticleScientificpeer-review

40 Citations (Scopus)
2001

A new technology for beer flavor stabilization: control of key intermediates of the Maillard reaction

Rangel-Aldao, R., Bravo, A., Galindo-Castro, I., Sanchez, B., Reverol, L., Scherer, E., Madrid, J., Ramirez, J. L., Herrera, J., Penttilä, M., Vehkomäki, M-L., Vidgren, V., Virtanen, H. & Home, S., 2001, Proceedings of the 28th EBC Congress: Budapest 2001. Nurnberg: Fachverlag Hans Carl, p. 576-584

Research output: Chapter in Book/Report/Conference proceedingConference article in proceedingsScientificpeer-review

Cellular responses to impaired protein folding and transport in Trichoderma reesei

Pakula, T., Riikonen, M., Huuskonen, A., Saloheimo, M., Valkonen, M., Uusitalo, J. & Penttilä, M., 2001.

Research output: Contribution to conferenceConference articleScientific

Development of brewers' yeast with increased carbonyl reducing activity

Vehkomäki, M-L., Vidgren, V., Vilpola, A., Virtanen, H., Sanchez, B., Galindo-Castsro, I., Penttilä, M. & Rangel-Aldao, R., 2001, Proceedings of the 28th EBC congress : Budapest 2001. Nurnberg: Fachverlag Hans Carl

Research output: Chapter in Book/Report/Conference proceedingConference article in proceedingsScientificpeer-review

Production of human tissue plasminogen activator in Trichoderma reesei

Uusitalo, J., Pakula, T., Bailey, M. & Penttilä, M., 2001.

Research output: Contribution to conferenceConference articleScientific

2002

13C-NMR flux ratio analysis of Saccharomyces cerevisiae growing on glucose or xylose in chemostat cultivations

Maaheimo, H., Pitkänen, J-P., Szyperski, T., Aristidou, A. & Penttilä, M., 2002.

Research output: Contribution to conferenceConference PosterScientific

13NMR flux ratio analysis of Saccharomyces cerevisiae growing on glucose or xylose in chemostat cultivations

Maaheimo, H., Pitkänen, J-P., Szyperski, T., Salusjärvi, L., Aristidou, A. & Penttilä, M., 2002.

Research output: Contribution to conferenceConference articleScientific

Maltoosin ja maltotrioosin kuljetus panimohiivakannoissa

Translated title of the contribution: Transport av maltos och maltotrios in i bryggerijäst; Maltose and maltotriose transport into brewer's yeastsVidgren, V., Ruohonen, L. & Londesborough, J., 2002, In : Mallas ja Olut. 4, p. 110-119

Research output: Contribution to journalArticle

Recombinant xylose utilizing Saccharomyces cerevisiae studied by genome wide approaches

Salusjärvi, L., Poutanen, M., Pitkänen, J-P., Koivistoinen, H., Aristidou, A., Kalkkinen, N., Ruohonen, L. & Penttilä, M., 2002. 1 p.

Research output: Contribution to conferenceConference articleScientific

The effect of cytosolic malic enzyme on anaerobic xylose fermentations

Rintala, E., Ruohonen, L., Londesborough, J., Uusitalo, J. & Penttilä, M., 2002.

Research output: Contribution to conferenceConference PosterScientific

Xylose utilisation by recombinant Saccharomyces cerevisiae

Rintala, E., Salusjärvi, L., Saloheimo, A., Toivari, M., Richard, P., Uusitalo, J., Londesborough, J., Ruohonen, L. & Penttilä, M., 2002, Biotechnology for Fuels and Chemicals: The Twenty-Fourth Symposium. Davison, B. H., Lee, J. W., McMillan, J. D. & Finkelstein, M. (eds.). Humana Press, 82. (Applied Biochemistry and Biotechnology (ABAB); No. 105-108).

Research output: Chapter in Book/Report/Conference proceedingConference article in proceedingsScientific

2003

Efficient cellulase production by Trichoderma reesei in continuous cultivation on lactose medium with a computer-controlled feeding strategy

Bailey, M. & Tähtiharju, J., 2003, In : Applied Microbiology and Biotechnology. 62, 2-3, p. 156-162 7 p.

Research output: Contribution to journalArticleScientificpeer-review

84 Citations (Scopus)

Investigation of the pentose phosphate pathway in Trichoderma reesei: disruption of the phosphoglucose isomerase gene

Limon, C., Uusitalo, J., Pakula, T., Saloheimo, M. & Penttilä, M., 2003, 22nd Fungal Genetics Conference at Asilomar: Abstracts from the 2003 Fungal Genetics Conference at Asilomar. 30. (Fungal Genetics Reports; No. Article 18, Vol. 50).

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsScientific

Open Access

Metabolic flux analysis of xylose metabolism in recombinant Saccharomyces cerevisiae using continuous culture

Pitkänen, J. P., Aristidou, A., Salusjärvi, L., Ruohonen, L. & Penttilä, M., 1 Jan 2003, In : Metabolic Engineering. 5, 1, p. 16-31 16 p.

Research output: Contribution to journalArticle

75 Citations (Scopus)

Proteome analysis of recombinant xylose-fermenting Saccharomyces cerevisiae

Salusjärvi, L., Poutanen, M., Pitkänen, J. P., Koivistoinen, H., Aristidou, A., Kalkkinen, N., Ruohonen, L. & Penttilä, M., 1 Mar 2003, In : Yeast. 20, 4, p. 295-314 20 p.

Research output: Contribution to journalArticleScientificpeer-review

47 Citations (Scopus)

Stable production of recombinant proteins in filamentous fungi: Problems and improvements

Wiebe, M., 2003, In : Mycologist. 17, 3, p. 140-144 5 p.

Research output: Contribution to journalArticleScientificpeer-review

21 Citations (Scopus)
103 Citations (Scopus)
2004

Evolution of recombinant (t-PA) Trichoderma reesei in chemostats selects mutants down-regulated in cellulase production

Lanthaler, K., Uusitalo, J., Pakula, T., Penttilä, M. & Robson, G. D., 2004, Poster Abstracts. p. 189 VIIIp-5

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsScientific

Open Access

Integrative approaches for studying pentose metabolism in Saccharomyces cerevisiae

Salusjärvi, L., Oresic, M., Pitkänen, J-P., Rintala, E., Koivistoinen, H., Ruohonen, L. & Penttilä, M., 2004.

Research output: Contribution to conferenceConference articleScientific

Proteome analysis in Trichoderma reesei

Pakula, T., Riipi, T., Koivistoinen, H., Lanthaler, K., Uusitalo, J., Saloheimo, M., Valmu, L., Kalkkinen, N., Robson, G. & Penttilä, M., 2004.

Research output: Contribution to conferenceConference articleScientific

Quorn™ myco-protein: Overview of a successful fungal product

Wiebe, M., 2004, In : Mycologist. 18, 1, p. 17 - 20 4 p.

Research output: Contribution to journalArticle

40 Citations (Scopus)