1988 …2019

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2019

Bioconjugation with Aminoalkylhydrazine for Efficient Mass Spectrometry-Based Detection of Small Carbonyl Compounds

Thangaraj, S. K., Voutilainen, S., Andberg, M., Koivula, A., Jänis, J. & Rouvinen, J., 20 Aug 2019, In : ACS Omega. 4, 8, p. 13447 - 13453 7 p.

Research output: Contribution to journalArticleScientificpeer-review

Open Access
1 Citation (Scopus)

Cloning of novel bacterial xylanases from lignocellulose-enriched compost metagenomic libraries

Ellilä, S., Bromann, P., Nyyssönen, M., Itävaara, M., Koivula, A., Paulin, L. & Kruus, K., 2019, In : AMB Express. 9, 1, 124.

Research output: Contribution to journalArticleScientificpeer-review

Open Access
2 Citations (Scopus)

In vitro reconstitution and characterisation of the oxidative d-xylose pathway for production of organic acids and alcohols

Boer, H., Andberg, M., Pylkkänen, R., Maaheimo, H. & Koivula, A., 2019, In : AMB Express. 9, 1, 48.

Research output: Contribution to journalArticleScientificpeer-review

Open Access
2 Citations (Scopus)
2018

A glucose/oxygen enzymatic fuel cell based on gold nanoparticles modified graphene screen-printed electrode. Proof-of-concept in human saliva

Bollella, P., Fusco, G., Stevar, D., Gorton, L., Ludwig, R., Ma, S., Boer, H., Koivula, A., Tortolini, C., Favero, G., Antiochia, R. & Mazzei, F., 2018, In : Sensors and Actuators B: Chemical. 256, p. 921-930 10 p.

Research output: Contribution to journalArticleScientificpeer-review

35 Citations (Scopus)

The crystal structure of D-xylonate dehydratase reveals functional features of enzymes from the Ilv/ED dehydratase family

Rahman, M. M., Andberg, M., Koivula, A., Rouvinen, J. & Hakulinen, N., 1 Dec 2018, In : Scientific Reports. 8, 1, 865.

Research output: Contribution to journalArticleScientificpeer-review

Open Access
8 Citations (Scopus)
2017

The Crystal Structure of a Bacterial l -Arabinonate Dehydratase Contains a [2Fe-2S] Cluster

Rahman, M., Andberg, M., Thangaraj, S., Parkkinen, T., Penttilä, M., Jänis, J., Koivula, A., Rouvinen, J. & Hakulinen, N., 21 Jul 2017, In : ACS Chemical Biology. 12, 7, p. 1919-1927 9 p.

Research output: Contribution to journalArticleScientificpeer-review

10 Citations (Scopus)
2016

Characterization and mutagenesis of two novel iron-sulphur cluster pentonate dehydratases

Andberg, M., Aro-Kärkkäinen, N., Carlson, P., Oja, M., Bozonnet, S., Toivari, M., Hakulinen, N., O'Donohue, M., Penttilä, M. & Koivula, A., 2016, In : Applied Microbiology and Biotechnology. 100, 17, p. 7549-7563

Research output: Contribution to journalArticleScientificpeer-review

17 Citations (Scopus)

Characterization of a unique Caulobacter crescentus aldose-aldose oxidoreductase having dual activities

Andberg, M., Maaheimo, H., Kumpula, E-P., Boer, H., Toivari, M., Penttilä, M. & Koivula, A., 2016, In : Applied Microbiology and Biotechnology. 100, 2, p. 673-685

Research output: Contribution to journalArticleScientificpeer-review

2 Citations (Scopus)
Open Access
3 Citations (Scopus)

Expression of Two Novel β-Glucosidases from Chaetomium atrobrunneum in Trichoderma reesei and Characterization of the Heterologous Protein Products

Colabardini, A. C., Valkonen, M., Huuskonen, A., Siika-aho, M., Koivula, A., Goldman, G. H. & Saloheimo, M., 1 Dec 2016, In : Molecular Biotechnology. 58, 12, p. 821-831 11 p.

Research output: Contribution to journalArticleScientificpeer-review

11 Citations (Scopus)

Production and applications of carbohydrate-derived sugar acids as generic biobased chemicals

Mehtiö, T., Toivari, M., Wiebe, M. G., Harlin, A., Penttilä, M. & Koivula, A., 2016, In : Critical Reviews in Biotechnology. 36, 5, p. 904-916

Research output: Contribution to journalArticleScientificpeer-review

28 Citations (Scopus)
2015

A novel aldose-aldose oxidoreductase for co-production of D-xylonate and xylitol from D-xylose with Saccharomyces cerevisiae

Wiebe, M. G., Nygård, Y., Oja, M., Andberg, M., Ruohonen, L., Koivula, A., Penttilä, M. & Toivari, M., 2015, In : Applied Microbiology and Biotechnology. 99, 22, p. 9439-9447

Research output: Contribution to journalArticleScientificpeer-review

Open Access
11 Citations (Scopus)

Structure and function of Caulobacter crescentus aldose-aldose oxidoreductase

Taberman, H., Andberg, M., Koivula, A., Hakulinen, N., Penttilä, M., Rouvinen, J. & Parkkinen, T., 2015, In : Biochemical Journal. 472, 3, p. 297-307

Research output: Contribution to journalArticleScientificpeer-review

8 Citations (Scopus)
2014

Engineering chimeric thermostable GH7 cellobiohydrolases in Saccharomyces cerevisiae

Voutilainen, S. P., Nurmi-Rantala, S., Penttilä, M. & Koivula, A., 1 Jan 2014, In : Applied Microbiology and Biotechnology. 98, 7, p. 2991-3001

Research output: Contribution to journalArticleScientificpeer-review

25 Citations (Scopus)

l-Arabinose/d-galactose 1-dehydrogenase of Rhizobium leguminosarum bv. trifolii characterised and applied for bioconversion of l-arabinose to l-arabonate with Saccharomyces cerevisiae

Aro-Kärkkäinen, N., Toivari, M., Maaheimo, H., Ylilauri, M., Pentikäinen, O. T., Andberg, M., Oja, M., Penttilä, M., Wiebe, M. G., Ruohonen, L. & Koivula, A., 1 Jan 2014, In : Applied Microbiology and Biotechnology. 98, 23, p. 9653-9665 13 p.

Research output: Contribution to journalArticleScientificpeer-review

9 Citations (Scopus)

Purification, crystallization and preliminary X-ray diffraction analysis of a novel keto-deoxy-D-galactarate (KDG) dehydratase from Agrobacterium tumefaciens

Taberman, H., Andberg, M., Parkkinen, T., Richard, P., Hakulinen, N., Koivula, A. & Rouvinen, J., 2014, In : Acta Crystallographica Section F: Structural Biology Communications. 70, p. 49-52 4 p.

Research output: Contribution to journalArticleScientificpeer-review

5 Citations (Scopus)

Single-molecule imaging analysis of elementary reaction steps of Trichoderma reesei cellobiohydrolase I (Cel7A) hydrolyzing crystalline cellulose Ia and IIII

Shibafuji, Y., Nakamura, A., Uchihashi, T., Sugimoto, N., Fukuda, S., Watanabe, H., Samejima, M., Ando, T., Noji, H., Koivula, A., Igarashi, K. & Iino, R., 2014, In : Journal of Biological Chemistry. 289, 20, p. 14056-14065 10 p.

Research output: Contribution to journalArticleScientificpeer-review

30 Citations (Scopus)

Structure and function of a decarboxylating Agrobacterium tumefaciens Keto-deoxy-D-galactarate dehydratase

Taberman, H., Andberg, M., Parkkinen, T., Jänis, J., Penttilä, M., Hakulinen, N., Koivula, A. & Rouvinen, J., 30 Dec 2014, In : Biochemistry. 53, 51, p. 8052-8060 9 p.

Research output: Contribution to journalArticleScientificpeer-review

8 Citations (Scopus)
2013

Effect of temperature on lignin-derived inhibition studied with three structurally different cellobiohydrolases

Rahikainen, J., Moilanen, U., Nurmi-Rantala, S., Lappas, A., Koivula, A., Viikari, L. & Kruus, K., 2013, In : Bioresource Technology. 146, p. 118-125 7 p.

Research output: Contribution to journalArticleScientificpeer-review

32 Citations (Scopus)

The Tryptophan Residue at the Active Site Tunnel Entrance of Trichoderma reesei Cellobiohydrolase Cel7A Is Important for Initiation of Degradation of Crystalline Cellulose

Nakamura, A., Tsukada, T., Auer, S., Furuta, T., Wada, M., Koivula, A., Igarashi, K. & Samejima, M., 2013, In : Journal of Biological Chemistry. 288, 19, p. 13503-13510 8 p.

Research output: Contribution to journalArticleScientificpeer-review

53 Citations (Scopus)
2012
24 Citations (Scopus)

Engineering chitinases for the synthesis of chitin oligosaccharides: Catalytic amino acid mutations convert the GH-18 family glycoside hydrolases into transglycosylases

Martinez, E. A., Boer, H., Koivula, A., Samain, E., Driguez, H., Armand, S. & Cottaz, S., 2012, In : Journal of Molecular Catalysis B: Enzymatic. 74, 1-2, p. 89-96 8 p.

Research output: Contribution to journalArticleScientificpeer-review

28 Citations (Scopus)

Lignocellulosic ethanol: From science to industry

Viikari, L., Vehmaanperä, J. & Koivula, A., 2012, In : Biomass and Bioenergy. 46, p. 13-24 11 p.

Research output: Contribution to journalArticleScientificpeer-review

166 Citations (Scopus)

Metabolic engineering of Saccharomyces cerevisiae for bioconversion of d-xylose to d-xylonate

Toivari, M., Nygård, Y., Kumpula, E. P., Vehkomäki, M. L., Benčina, M., Valkonen, M., Maaheimo, H., Andberg, M., Koivula, A., Ruohonen, L., Penttilä, M. & Wiebe, M. G., 1 Jul 2012, In : Metabolic Engineering. 14, 4, p. 427-436

Research output: Contribution to journalArticleScientificpeer-review

Open Access
51 Citations (Scopus)

Visualization of cellobiohydrolase i from trichoderma reesei moving on crystalline cellulose using high-speed atomic force microscopy

Igarashi, K., Uchihashi, T., Koivula, A., Wada, M., Kimura, S., Penttilä, M., Ando, T. & Samejima, M., 22 May 2012, In : Methods in Enzymology. 510, p. 169-182

Research output: Contribution to journalArticleScientificpeer-review

15 Citations (Scopus)
2011

Crystal structure of an ascomycete fungal laccase from Thielavia arenaria : Common structural features of asco-laccases

Kallio, J. P., Gasparetti, C., Andberg, M., Boer, H., Koivula, A., Kruus, K., Rouvinen, J. & Hakulinen, N., 2011, In : FEBS Journal. 278, 13, p. 2283-2295 14 p.

Research output: Contribution to journalArticleScientificpeer-review

Open Access
49 Citations (Scopus)

Crystal structure of uronate dehydrogenase from Agrobacterium tumefaciens

Parkkinen, T., Boer, H., Jänis, J., Andberg, M., Penttilä, M., Koivula, A. & Rouvinen, J., 5 Aug 2011, In : Journal of Biological Chemistry. 286, 31, p. 27294-27300

Research output: Contribution to journalArticleScientificpeer-review

25 Citations (Scopus)

High level secretion of cellobiohydrolases by Saccharomyces cerevisiae

Ilmén, M., Den Haan, R., Brevnova, E., McBride, J., Wiswall, E., Froehlich, A., Koivula, A., Voutilainen, S. P., Siika-Aho, M., La Grange, D. C., Thorngren, N., Ahlgren, S., Mellon, M., Deleault, K., Rajgarhia, V., Van Zyl, W. H. & Penttilä, M., 14 Sep 2011, In : Biotechnology for Biofuels. 4, 30.

Research output: Contribution to journalArticleScientificpeer-review

Open Access
107 Citations (Scopus)

Traffic jams reduce hydrolytic efficiency of cellulase on cellulose surface

Igarashi, K., Uchihashi, T., Koivula, A., Wada, M., Kimura, S., Okamoto, T., Penttilä, M., Ando, T. & Samejima, M., 2 Sep 2011, In : Science. 333, 6047, p. 1279-1282

Research output: Contribution to journalArticleScientificpeer-review

364 Citations (Scopus)
2010

Electrochemical evaluation of electron transfer kinetics of high and low redox potential laccases on gold electrode surface

Frasconi, M., Boer, H., Koivula, A. & Mazzei, F., 2010, In : Electrochimica Acta. 56, 2, p. 817-827 11 p.

Research output: Contribution to journalArticleScientificpeer-review

29 Citations (Scopus)

Expression of Talaromyces emersonii cellobiohydrolase Cel7A in Saccharomyces cerevisiae and rational mutagenesis to improve its thermostability and activity

Voutilainen, S., Murray, P. G., Tuohy, M. G. & Koivula, A., 2010, In : Protein Engineering, Design and Selection. 23, 2, p. 69-79 11 p.

Research output: Contribution to journalArticleScientificpeer-review

93 Citations (Scopus)

Identification in Agrobacterium tumefaciens of the D-galacturonic acid dehydrogenase gene

Boer, H., Maaheimo, H., Koivula, A., Penttilä, M. & Richard, P., 1 Apr 2010, In : Applied Microbiology and Biotechnology. 86, 3, p. 901-909

Research output: Contribution to journalArticleScientificpeer-review

37 Citations (Scopus)

Kinetic and biochemical properties of high and low redox potential laccases from fungal and plant origin

Frasconi, M., Favero, G., Boer, H., Koivula, A. & Mazzei, F., 2010, In : Biochimica et Biophysica Acta: Proteins and Proteomics. 1804, 4, p. 899-908 10 p.

Research output: Contribution to journalArticleScientificpeer-review

66 Citations (Scopus)

Performance of a printable enzymatic fuel cell: Study on mediated ThL laccase cathode

Tuurala, S., Smolander, M., Uotila, J., Kaukoniemi, O-V., Boer, H., Valkiainen, M., Vaari, A., Koivula, A. & Jenkins, P., 2010, In : ECS Transactions. 25, 33, p. 1-10 10 p.

Research output: Contribution to journalArticleScientificpeer-review

4 Citations (Scopus)
2009

Characterisation of the wheat germ agglutinin binding to self-assembled monolayers of neoglycoconjugates by AFM and SPR

Lienemann, M., Paananen, A., Boer, H., de la Fuente, J. M., García, I., Penadés, S. & Koivula, A., 24 Feb 2009, In : Glycobiology. 19, 6, p. 633 - 643

Research output: Contribution to journalArticleScientificpeer-review

21 Citations (Scopus)

Essential role of the C-terminus in Melanocarpus albomyces laccase for enzyme production, catalytic properties and structure

Andberg, M., Hakulinen, N., Auer, S., Saloheimo, M., Koivula, A., Rouvinen, J. & Kruus, K., 2009, In : FEBS Journal. 276, 21, p. 6285-6300 16 p.

Research output: Contribution to journalArticleScientificpeer-review

60 Citations (Scopus)

High speed atomic force microscopy visualizes processive movement of Trichoderma reesei cellobiohydrolase I on crystalline cellulose

Igarashi, K., Koivula, A., Wada, M., Kimura, S., Penttilä, M. & Samejima, M., 25 Dec 2009, In : Journal of Biological Chemistry. 284, 52, p. 36186-36190

Research output: Contribution to journalArticleScientificpeer-review

199 Citations (Scopus)

Improving the thermostability and activity of Melanocarpus albomyces cellobiohydrolase Cel7B

Voutilainen, S., Boer, H., Alapuranen, M., Jänis, J., Vehmaanperä, J. & Koivula, A., 2009, In : Applied Microbiology and Biotechnology. 83, 2, p. 261-272 12 p.

Research output: Contribution to journalArticleScientificpeer-review

44 Citations (Scopus)

Structure-function studies of a Melanocarpus albomyces laccase suggest a pathway for oxidation of phenolic compounds

Kallio, J. P., Auer, S., Jänis, J., Andberg, M., Kruus, K., Rouvinen, J., Koivula, A. & Hakulinen, N., 2009, In : Journal of Molecular Biology. 392, 4, p. 895-909 15 p.

Research output: Contribution to journalArticleScientificpeer-review

70 Citations (Scopus)

Toward understanding of carbohydrate binding and substrate specificity of a glycosyl hydrolase 18 family (GH-18) chitinase from Trichoderma harzianum

Lienemann, M., Boer, H., Paananen, A., Cottaz, S. & Koivula, A., 13 Jul 2009, In : Glycobiology. 19, 10, p. 1116 - 1126

Research output: Contribution to journalArticleScientificpeer-review

19 Citations (Scopus)
2008

A near atomic resolution structure of a Melanocarpus albomyces laccase

Hakulinen, N., Andberg, M., Kallio, J., Koivula, A., Kruus, K. & Rouvinen, J., 2008, In : Journal of Structural Biology. 162, 1, p. 29 - 39 11 p.

Research output: Contribution to journalArticleScientificpeer-review

54 Citations (Scopus)

Cloning, expression, and characterization of novel thermostable family 7 cellobiohydrolases

Voutilainen, S., Puranen, T., Siika-aho, M., Lappalainen, A., Alapuranen, M., Kallio, J., Hooman, S., Viikari, L., Vehmaanperä, J. & Koivula, A., 2008, In : Biotechnology and Bioengineering. 101, 3, p. 515-528 13 p.

Research output: Contribution to journalArticleScientificpeer-review

Open Access
81 Citations (Scopus)

Crystal structures of Melanocarpus albomyces cellobiohydrolase Cel7B in complex with cello‐oligomers show high flexibility in the substrate binding

Parkkinen, T., Koivula, A., Vehmaanperä, J. & Rouvinen, J., 2008, In : Protein Science. 17, 8, p. 1383 - 1394 12 p.

Research output: Contribution to journalArticleScientificpeer-review

38 Citations (Scopus)

Development of a printable laccase-based biocathode for fuel cell applications

Smolander, M., Boer, H., Valkiainen, M., Roozeman, R., Bergelin, M., Eriksson, J-E., Zhang, X-C., Koivula, A. & Viikari, L., 2008, In : Enzyme and Microbial Technology. 43, 2, p. 93 - 102 10 p.

Research output: Contribution to journalArticleScientificpeer-review

72 Citations (Scopus)
2007

Heterologous expression and site-directed mutagenesis studies of two Trichoderma harzianum chitinases, Chit33 and Chit42, in Escherichia coli

Boer, H., Simolin, H., Cottaz, S., Söderlund, H. & Koivula, A., 2007, In : Protein Expression and Purification. 51, 2, p. 216-226

Research output: Contribution to journalArticleScientificpeer-review

17 Citations (Scopus)

Heterologous expression of Melanocarpus albomyces cellobiohydrolase Cel7B, and random mutagenesis to improve its thermostability

Voutilainen, S., Boer, H., Linder, M., Puranen, T., Rouvinen, J., Vehmaanperä, J. & Koivula, A., 2007, In : Enzyme and Microbial Technology. 41, 3, p. 234-243

Research output: Contribution to journalArticleScientificpeer-review

35 Citations (Scopus)

Mutation of fungal endoglucanases into glycosynthases and characterization of their acceptor substrate specificity

Blanchard, S., Cottaz, S., Coutinho, P. M., Patkar, S., Vind, J., Boer, H., Koivula, A., Driguez, H. & Armand, S., 2007, In : Journal of Molecular Catalysis B: Enzymatic. 44, 3-4, p. 106-116

Research output: Contribution to journalArticleScientificpeer-review

5 Citations (Scopus)

Preliminary X-ray analysis of cellobiohydrolase Cel7B from Melanocarpus albomyces

Parkkinen, T., Koivula, A., Vehmaanperä, J. & Rouvinen, J., 2007, In : Acta Crystallographica Section F: Structural Biology and Crystallization Communications. F63, 9, p. 754-757

Research output: Contribution to journalArticleScientificpeer-review

4 Citations (Scopus)
2006

A crystallographic and spectroscopic study on the effect of X-ray radiation on the crystal structure of Melanocarpus albomyces laccase

Hakulinen, N., Kruus, K., Koivula, A. & Rouvinen, J., 2006, In : Biochemical and Biophysical Research Communications. 350, 4, p. 929-934 6 p.

Research output: Contribution to journalArticleScientificpeer-review

44 Citations (Scopus)

Crystal structures of an enantioselective Fab-fragment in free and complex forms

Parkkinen, T., Nevanen, T., Koivula, A. & Rouvinen, J., 2006, In : Journal of Molecular Biology. 357, 2, p. 471-480

Research output: Contribution to journalArticleScientificpeer-review

15 Citations (Scopus)