@inbook{5b11270ce4d54d0f912e71e0e060f437,
title = "α-Amylases: Interaction with Polysaccharide Substrates, Proteinaceous Inhibitors and Regulatory Proteins",
abstract = "α-Amylases occur widely in plants, animals, and microorganisms. They often act in synergy with other related and degradative enzymes and may also be regulated by proteinaceous inhibitors. Open questions exist on how αamylases interact with polysaccharides. Several enzymes possess secondary carbohydrate binding sites situated on the surface at a certain distance of the active site cleft. The functions of such sites were studied in barley αamylase isozymes by structure-guided mutational analysis and measurement of activity and binding parameters. Two surface sites were assigned distinct roles. One of the sites seems to participate in hydrolysis of polysaccharides by a multiple attack mechanism. Polysaccharide processing enzymes can also contain carbohydrate binding modules, e.g. starch binding domains that assist in the attack on macromolecular substrates and are useful in engineering of enzyme efficiency. The multidomain nature of these enzymes raises questions on the dynamics and structural properties in solution and in substrate complexes.",
author = "Seo, {E. S.} and Nielsen, {M. M.} and Andersen, {J. M.} and Vester-Christensen, {M. B.} and Jensen, {J. M.} and C. Christiansen and Adiphol Dilokpimol and Hachem, {M. Abou} and P. H{\"a}gglund and K. Maedal and C. Finnie and A. Blennow and B. Svensson",
note = "Funding Information: The expert technical assistance of Susanne Blume and Karina Rasmussen is gratefully acknowledged. The work has been supported by the Danish Natural Science Research Council, the Danish Research Council for Technology and Production Sciences and the Carlsberg Foundation. ESS held a Korea Research Foundation Grant funded by the Korean Government (MOEHRD) (KRF-2005-214-D00275) and a H.C. {\O}rsted postdoctoral fellowship from DTU. MMN and MB VC received Ph.D. stipends from DTU. CC holds a Ph.D. stipend from the FOBI graduate school. JMA received a Novo student scholarship.",
year = "2008",
month = sep,
doi = "10.1533/9781845695750.1.20",
language = "English",
isbn = "978-1-84569-519-4",
pages = "20--36",
editor = "Kwan-Hwa Park",
booktitle = "Carbohydrate-Active Enzymes",
publisher = "Woodhead Publishing",
address = "United Kingdom",
}