TY - JOUR
T1 - β-1,3-Glucan synthesis, novel supramolecular self-assembly, characterization and application
AU - Pylkkänen, Robert
AU - Mohammadi, Pezhman
AU - Liljeström, Ville
AU - Płaziński, Wojciech
AU - Beaune, Grégory
AU - Timonen, Jaakko V. I.
AU - Penttilä, Merja
N1 - Publisher Copyright:
© 2022 The Royal Society of Chemistry.
PY - 2022/10/4
Y1 - 2022/10/4
N2 - β-1,3-Glucans are ubiquitously observed in various biological systems with diverse physio-ecological functions, yet their underlying assembly mechanism and multiscale complexation in vitro remains poorly understood. Here, we provide for the first-time evidence of unidentified β-1,3-glucan supramolecular complexation into intricate hierarchical architectures over several length scales. We mediated these unique assemblies using a recombinantly produced β-1,3-glucan phosphorylase (Ta1,3BGP) by fine-tuning solution conditions during particle nucleation and growth. We report a synthesis of interconnected parallel hexagonal lamellae composed of 8 nm thick sheets of highly expanded paracrystals. The architecture consists of β-1,3-glucan triple-helices with considerable inter–intra hydrogen bonding within, as well as in between adjacent triple-helices. The results extend our understanding of β-1,3-glucan molecular organization and shed light on different aspects of the crystallization processes of biomolecules into structures unseen by nature. The presented versatile synthesis yields new materials for diverse medical and industrial applications.
AB - β-1,3-Glucans are ubiquitously observed in various biological systems with diverse physio-ecological functions, yet their underlying assembly mechanism and multiscale complexation in vitro remains poorly understood. Here, we provide for the first-time evidence of unidentified β-1,3-glucan supramolecular complexation into intricate hierarchical architectures over several length scales. We mediated these unique assemblies using a recombinantly produced β-1,3-glucan phosphorylase (Ta1,3BGP) by fine-tuning solution conditions during particle nucleation and growth. We report a synthesis of interconnected parallel hexagonal lamellae composed of 8 nm thick sheets of highly expanded paracrystals. The architecture consists of β-1,3-glucan triple-helices with considerable inter–intra hydrogen bonding within, as well as in between adjacent triple-helices. The results extend our understanding of β-1,3-glucan molecular organization and shed light on different aspects of the crystallization processes of biomolecules into structures unseen by nature. The presented versatile synthesis yields new materials for diverse medical and industrial applications.
UR - http://www.scopus.com/inward/record.url?scp=85140933188&partnerID=8YFLogxK
U2 - 10.1039/D2NR02731C
DO - 10.1039/D2NR02731C
M3 - Article
SN - 2040-3364
VL - 14
SP - 15533
EP - 15541
JO - Nanoscale
JF - Nanoscale
IS - 41
ER -