β-Lactamase Production in Prevotella intermedia, Prevotella nigrescens, and Prevotella pallens Genotypes and In Vitro Susceptibilities to Selected Antimicrobial Agents

Jaana Mättö (Corresponding Author), Sirkka Asikainen, Marja-Liisa Väisänen, Birgitta Von Troil-Lindén, Eija Könönen, Maria Saarela, Kari Salminen, Sydney M. Finegold, Hannele Jousimies-Somer

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Abstract

The present study investigated the β-lactamase production of 73 Prevotella intermedia, 84 Prevotella nigrescens, and 14 Prevotella pallens isolates and their in vitro susceptibilities to six antimicrobial agents. The P. intermedia and P. nigrescens isolates were recovered from oral and extraoral samples obtained from subjects in two geographic locations from 1985 to 1995. The clonality of the β-lactamase-positive and β-lactamase-negative isolates and the clustering of the genotypes were studied by arbitrarily primed-PCR fingerprinting. β-Lactamase production was detected in 29% of P. intermedia isolates, 29% of P. nigrescens isolates, and 57% of P. pallens isolates. No difference in the frequencies of β-lactamase production by P. intermedia and P. nigrescens between isolates from oral and extraoral sites, between isolates obtained at different time periods, or between P. intermedia isolates from different geographic locations was observed. However, the P. nigrescens isolates from the United States were significantly more frequently (P = 0.015) β-lactamase positive than those from Finland. No association between the genotypes and β-lactamase production or between the genotypes and the sources of the isolates was found. The penicillin G MICs at which 90% of the isolates were inhibited were 8 μg/ml for P. intermedia, 8 μg/ml for P. nigrescens, and 16 μg/ml for P. pallens. For the β-lactamase-negative isolates, the corresponding values were 0.031, 0.031, and 0.125 μg/ml, and for the β-lactamase-positive isolates, the corresponding values were 16, 8, and 32 μg/ml. All isolates were susceptible to amoxicillin-clavulanate, cefoxitin, metronidazole, azithromycin, and trovafloxacin. The MICs of amoxicillin-clavulanate and cefoxitin were relatively higher for the β-lactamase-positive population than for the β-lactamase-negative population.
Original languageEnglish
Pages (from-to)2383-2388
Number of pages6
JournalAntimicrobial Agents and Chemotherapy
Volume43
Issue number10
DOIs
Publication statusPublished - 1999
MoE publication typeA1 Journal article-refereed

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Prevotella nigrescens
Prevotella intermedia
Prevotella
Anti-Infective Agents
Genotype
Cefoxitin
Geographic Locations
Clavulanic Acid
Amoxicillin
Azithromycin
Penicillin G
Metronidazole
Finland
Population
Cluster Analysis
In Vitro Techniques
Polymerase Chain Reaction

Cite this

Mättö, Jaana ; Asikainen, Sirkka ; Väisänen, Marja-Liisa ; Von Troil-Lindén, Birgitta ; Könönen, Eija ; Saarela, Maria ; Salminen, Kari ; Finegold, Sydney M. ; Jousimies-Somer, Hannele. / β-Lactamase Production in Prevotella intermedia, Prevotella nigrescens, and Prevotella pallens Genotypes and In Vitro Susceptibilities to Selected Antimicrobial Agents. In: Antimicrobial Agents and Chemotherapy. 1999 ; Vol. 43, No. 10. pp. 2383-2388.
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title = "β-Lactamase Production in Prevotella intermedia, Prevotella nigrescens, and Prevotella pallens Genotypes and In Vitro Susceptibilities to Selected Antimicrobial Agents",
abstract = "The present study investigated the β-lactamase production of 73 Prevotella intermedia, 84 Prevotella nigrescens, and 14 Prevotella pallens isolates and their in vitro susceptibilities to six antimicrobial agents. The P. intermedia and P. nigrescens isolates were recovered from oral and extraoral samples obtained from subjects in two geographic locations from 1985 to 1995. The clonality of the β-lactamase-positive and β-lactamase-negative isolates and the clustering of the genotypes were studied by arbitrarily primed-PCR fingerprinting. β-Lactamase production was detected in 29{\%} of P. intermedia isolates, 29{\%} of P. nigrescens isolates, and 57{\%} of P. pallens isolates. No difference in the frequencies of β-lactamase production by P. intermedia and P. nigrescens between isolates from oral and extraoral sites, between isolates obtained at different time periods, or between P. intermedia isolates from different geographic locations was observed. However, the P. nigrescens isolates from the United States were significantly more frequently (P = 0.015) β-lactamase positive than those from Finland. No association between the genotypes and β-lactamase production or between the genotypes and the sources of the isolates was found. The penicillin G MICs at which 90{\%} of the isolates were inhibited were 8 μg/ml for P. intermedia, 8 μg/ml for P. nigrescens, and 16 μg/ml for P. pallens. For the β-lactamase-negative isolates, the corresponding values were 0.031, 0.031, and 0.125 μg/ml, and for the β-lactamase-positive isolates, the corresponding values were 16, 8, and 32 μg/ml. All isolates were susceptible to amoxicillin-clavulanate, cefoxitin, metronidazole, azithromycin, and trovafloxacin. The MICs of amoxicillin-clavulanate and cefoxitin were relatively higher for the β-lactamase-positive population than for the β-lactamase-negative population.",
author = "Jaana M{\"a}tt{\"o} and Sirkka Asikainen and Marja-Liisa V{\"a}is{\"a}nen and {Von Troil-Lind{\'e}n}, Birgitta and Eija K{\"o}n{\"o}nen and Maria Saarela and Kari Salminen and Finegold, {Sydney M.} and Hannele Jousimies-Somer",
year = "1999",
doi = "10.1128/AAC.43.10.2383",
language = "English",
volume = "43",
pages = "2383--2388",
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Mättö, J, Asikainen, S, Väisänen, M-L, Von Troil-Lindén, B, Könönen, E, Saarela, M, Salminen, K, Finegold, SM & Jousimies-Somer, H 1999, 'β-Lactamase Production in Prevotella intermedia, Prevotella nigrescens, and Prevotella pallens Genotypes and In Vitro Susceptibilities to Selected Antimicrobial Agents', Antimicrobial Agents and Chemotherapy, vol. 43, no. 10, pp. 2383-2388. https://doi.org/10.1128/AAC.43.10.2383

β-Lactamase Production in Prevotella intermedia, Prevotella nigrescens, and Prevotella pallens Genotypes and In Vitro Susceptibilities to Selected Antimicrobial Agents. / Mättö, Jaana (Corresponding Author); Asikainen, Sirkka; Väisänen, Marja-Liisa; Von Troil-Lindén, Birgitta; Könönen, Eija; Saarela, Maria; Salminen, Kari; Finegold, Sydney M.; Jousimies-Somer, Hannele.

In: Antimicrobial Agents and Chemotherapy, Vol. 43, No. 10, 1999, p. 2383-2388.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - β-Lactamase Production in Prevotella intermedia, Prevotella nigrescens, and Prevotella pallens Genotypes and In Vitro Susceptibilities to Selected Antimicrobial Agents

AU - Mättö, Jaana

AU - Asikainen, Sirkka

AU - Väisänen, Marja-Liisa

AU - Von Troil-Lindén, Birgitta

AU - Könönen, Eija

AU - Saarela, Maria

AU - Salminen, Kari

AU - Finegold, Sydney M.

AU - Jousimies-Somer, Hannele

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N2 - The present study investigated the β-lactamase production of 73 Prevotella intermedia, 84 Prevotella nigrescens, and 14 Prevotella pallens isolates and their in vitro susceptibilities to six antimicrobial agents. The P. intermedia and P. nigrescens isolates were recovered from oral and extraoral samples obtained from subjects in two geographic locations from 1985 to 1995. The clonality of the β-lactamase-positive and β-lactamase-negative isolates and the clustering of the genotypes were studied by arbitrarily primed-PCR fingerprinting. β-Lactamase production was detected in 29% of P. intermedia isolates, 29% of P. nigrescens isolates, and 57% of P. pallens isolates. No difference in the frequencies of β-lactamase production by P. intermedia and P. nigrescens between isolates from oral and extraoral sites, between isolates obtained at different time periods, or between P. intermedia isolates from different geographic locations was observed. However, the P. nigrescens isolates from the United States were significantly more frequently (P = 0.015) β-lactamase positive than those from Finland. No association between the genotypes and β-lactamase production or between the genotypes and the sources of the isolates was found. The penicillin G MICs at which 90% of the isolates were inhibited were 8 μg/ml for P. intermedia, 8 μg/ml for P. nigrescens, and 16 μg/ml for P. pallens. For the β-lactamase-negative isolates, the corresponding values were 0.031, 0.031, and 0.125 μg/ml, and for the β-lactamase-positive isolates, the corresponding values were 16, 8, and 32 μg/ml. All isolates were susceptible to amoxicillin-clavulanate, cefoxitin, metronidazole, azithromycin, and trovafloxacin. The MICs of amoxicillin-clavulanate and cefoxitin were relatively higher for the β-lactamase-positive population than for the β-lactamase-negative population.

AB - The present study investigated the β-lactamase production of 73 Prevotella intermedia, 84 Prevotella nigrescens, and 14 Prevotella pallens isolates and their in vitro susceptibilities to six antimicrobial agents. The P. intermedia and P. nigrescens isolates were recovered from oral and extraoral samples obtained from subjects in two geographic locations from 1985 to 1995. The clonality of the β-lactamase-positive and β-lactamase-negative isolates and the clustering of the genotypes were studied by arbitrarily primed-PCR fingerprinting. β-Lactamase production was detected in 29% of P. intermedia isolates, 29% of P. nigrescens isolates, and 57% of P. pallens isolates. No difference in the frequencies of β-lactamase production by P. intermedia and P. nigrescens between isolates from oral and extraoral sites, between isolates obtained at different time periods, or between P. intermedia isolates from different geographic locations was observed. However, the P. nigrescens isolates from the United States were significantly more frequently (P = 0.015) β-lactamase positive than those from Finland. No association between the genotypes and β-lactamase production or between the genotypes and the sources of the isolates was found. The penicillin G MICs at which 90% of the isolates were inhibited were 8 μg/ml for P. intermedia, 8 μg/ml for P. nigrescens, and 16 μg/ml for P. pallens. For the β-lactamase-negative isolates, the corresponding values were 0.031, 0.031, and 0.125 μg/ml, and for the β-lactamase-positive isolates, the corresponding values were 16, 8, and 32 μg/ml. All isolates were susceptible to amoxicillin-clavulanate, cefoxitin, metronidazole, azithromycin, and trovafloxacin. The MICs of amoxicillin-clavulanate and cefoxitin were relatively higher for the β-lactamase-positive population than for the β-lactamase-negative population.

U2 - 10.1128/AAC.43.10.2383

DO - 10.1128/AAC.43.10.2383

M3 - Article

VL - 43

SP - 2383

EP - 2388

JO - Antimicrobial Agents and Chemotherapy

JF - Antimicrobial Agents and Chemotherapy

SN - 0066-4804

IS - 10

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