1H NMR at 800 MHz facilitates detailed phospholipid follow-up during atherogenic modifications in low density lipoproteins

Pasi Soininen, K. Öörni, Hannu Maaheimo, R. Laatikainen, P. T. Kovanen, K. Kaski, M. Ala-Korpela (Corresponding Author)

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Abstract

The structure of low density lipoprotein (LDL) particles and, particularly, the enzymatic and oxidative modifications of their surface is crucial in the initiation of atherosclerosis. Due to the structural complexity of LDL, there is a lack of suitable methods for dynamic follow-up studies of the molecular mechanisms in native and modified particles in physiological conditions. Here, we report that phosphatidylcholine (PC), lysophosphatidylcholine (lyso-PC), and sphingomyelin (SM) can all be identified and quantified in LDL particles by 1H NMR spectroscopy at 800 MHz. The signal assignment for the lyso-PC is novel and we illustrate the applicability of the methodology in the case of lipid peroxidation that is generally considered as one of the key proatherogenic modifications of LDL. It was found, somewhat surprisingly, that the LDL-associated phospholipase A2 is activated in the very beginning of the formation of PC-hydroperoxides. The (patho)physiological rationale of the resulting lyso-PC generation is also briefly discussed.
Original languageEnglish
Pages (from-to)290-294
JournalBiochemical and Biophysical Research Communications
Volume360
Issue number1
DOIs
Publication statusPublished - 2007
MoE publication typeA1 Journal article-refereed

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Keywords

  • LDL
  • Lipoproteins
  • NMR spectroscopy
  • Phosphatidylcholine
  • Lysophosphatidylcholine
  • Sphingomyelin
  • Phospholipase A2
  • Lipoprotein-associated phospholipase A2
  • Lipid peroxidation
  • Atherosclerosis
  • Lipoprotein accumulation
  • Lipoprotein modifications

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