3-D printed meat alternatives based on pea and single cell proteins and hydrocolloids: effect of paste formulation on process-induced fibre alignment and structural and textural properties

Extrusion-based 3D food printing can be used as an alternative structuring technique to traditional extrusion processing for creating meat-like structures. This study focused on 3-D food printing to generate structures analogous to meat by using various combinations of texturized pea protein fibrils, microbial Single Cell Protein (SCP) and hydrocolloids locust bean gum and/or sodium alginate. Simple moulding was utilized as benchmarking to better understand the 3D printing-induced structural effects. To gain understanding of the interactions between proteins of different origin (plant and SCP) and with hydrocolloids, structural, textural and rheological properties were analysed. Oscillatory stress sweeps of all printing pastes revealed elastic-dominant rheological behaviour (G’ 4000-6000 Pa) with a defined yield stress (25-60 Pa) explaining their printability and shape stability. X-ray microtomography of ion-crosslinked analogues showed a printing-induced preferential alignment of fibrils in the direction of nozzle movement, while moulding led to a random orientation. Textural characterization via bi-directional cutting tests demonstrated higher cutting force in transversal (FT) over longitudinal (FL) direction in 3D-printed samples and equal forces in moulded samples. The anisotropy index (AI=FT/FL) of printed samples ranged between 1.4-2.5, indicating anisotropic texture, and 0.8-1 for moulded samples indicating isotropic texture. This study demonstrated the applicability of paste-extrusion in generating anisotropic structures analogous to meat by process-induced fibril alignment. The results support further development of 3D food printing technology in design of sustainable meat alternatives resembling whole-muscle meat.