Abstract
The introduction of a three dimensional scaffold
providing the closest analogies to extracellular matrix
(ECM) is currently a key strategy for tackling many
challenges in tissue repair. Here, we present a new
hybrid scaffold constructed by coating electrospun
chitosan/polyethylene oxide (PEO) nanofibers on
commercial BioTek polystyrene (PS) scaffold obtained from
Sigma Aldrich. The viability and proliferation rate of
mesenchymal stem cells (MSCs) seeded on micro-nano
structured hybrid scaffold (MNHS) and commercial PS
scaffolds were analyzed by MTT assay. The results of the
MTT assay revealed a higher degree of viability and
proliferation rate in MSCs seeded on MNHS compared with
the commercial PS scaffold. DAPI images also confirmed
the higher degree of attachment and viability of MSCs
seeded on MNHS. Moreover, MSCs on both scaffolds
differentiated to osteoblasts and adipocytes cells, as
reflected by the images obtained from Alizarin Red and
Oil Red-O staining. Alkaline phosphatase activity (ALP)
and calcium content assays revealed that the MNHS has a
higher potential for osteogenic differentiation than the
commercial scaffold. To quantify the osteoblast and
adipocyte gene expression, quantitative RT-PCR was
carried out for MNHS, commercial scaffold and Tissue
culture polystyrene (TCPS). It was found that MNHS can
express a higher level of Runt-related transcription
factor 2 (Runx2), osteonectin and osteocalcin in
osteogenic differentiation as well as increased
expression of PPAR? and UCP-1 in adipogenic
differentiation. The enhancement of the attachment,
viability and proliferation as well as bi-lineage
differentiation may result from the biochemical and
structural analogies of MNHS to native ECM. Furthermore,
it was observed that biocompatible MNHS scaffold can
potentially be utilized as a suitable scaffold for bone
and connective tissue engineering.
Original language | English |
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Pages (from-to) | 9000-9007 |
Journal | Journal of Nanoscience and Nanotechnology |
Volume | 16 |
Issue number | 9 |
DOIs | |
Publication status | Published - 2016 |
MoE publication type | A1 Journal article-refereed |
Keywords
- adipogenic differentiation
- hybrid scaffold
- mesenchymal stem cell
- micro porosity
- nanofiber
- osteogenic differentiation