3D-printable bioactivated nanocellulose-alginate hydrogels

Research output: Contribution to journalArticleScientificpeer-review

61 Citations (Scopus)

Abstract

We describe herein a nanocellulose-alginate hydrogel suitable for 3D printing. The composition of the hydrogel was optimized based on material characterization methods and 3D printing experiments, and its behavior during the printing process was studied using computational fluid dynamics simulations. The hydrogel was biofunctionalized by the covalent coupling of an enhanced avidin protein to the cellulose nanofibrils. Ionic cross-linking of the hydrogel using calcium ions improved the performance of the material. The resulting hydrogel is suitable for 3D printing, its mechanical properties indicate good tissue compatibility, and the hydrogel absorbs water in moist conditions, suggesting potential in applications such as wound dressings. The biofunctionalization potential was shown by attaching a biotinylated fluorescent protein and a biotinylated fluorescent small molecule via avidin and monitoring the material using confocal microscopy. The 3D-printable bioactivated nanocellulose-alginate hydrogel offers a platform for the development of biomedical devices, wearable sensors, and drug-releasing materials.
Original languageEnglish
Pages (from-to)21959-21970
Number of pages12
JournalACS Applied Materials & Interfaces
Volume9
Issue number26
DOIs
Publication statusPublished - 2017
MoE publication typeA1 Journal article-refereed

Fingerprint

Hydrogels
Hydrogel
Alginate
Printing
Avidin
Proteins
Confocal microscopy
alginic acid
Cellulose
Calcium
Computational fluid dynamics
Ions
Tissue
Mechanical properties
Molecules
Water
Monitoring
Computer simulation
Chemical analysis
Pharmaceutical Preparations

Keywords

  • 3D printing
  • alginate
  • avidin
  • hydrogel
  • nanocellulose
  • wound healing
  • ProperTune

Cite this

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title = "3D-printable bioactivated nanocellulose-alginate hydrogels",
abstract = "We describe herein a nanocellulose-alginate hydrogel suitable for 3D printing. The composition of the hydrogel was optimized based on material characterization methods and 3D printing experiments, and its behavior during the printing process was studied using computational fluid dynamics simulations. The hydrogel was biofunctionalized by the covalent coupling of an enhanced avidin protein to the cellulose nanofibrils. Ionic cross-linking of the hydrogel using calcium ions improved the performance of the material. The resulting hydrogel is suitable for 3D printing, its mechanical properties indicate good tissue compatibility, and the hydrogel absorbs water in moist conditions, suggesting potential in applications such as wound dressings. The biofunctionalization potential was shown by attaching a biotinylated fluorescent protein and a biotinylated fluorescent small molecule via avidin and monitoring the material using confocal microscopy. The 3D-printable bioactivated nanocellulose-alginate hydrogel offers a platform for the development of biomedical devices, wearable sensors, and drug-releasing materials.",
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author = "Jenni Leppiniemi and Panu Lahtinen and Antti Paajanen and Riitta Mahlberg and Sini Mets{\"a}-Kortelainen and Tatu Pinomaa and Heikki Pajari and Inger Vikholm-Lundin and Pekka Pursula and Vesa Hyt{\"o}nen",
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3D-printable bioactivated nanocellulose-alginate hydrogels. / Leppiniemi, Jenni; Lahtinen, Panu; Paajanen, Antti; Mahlberg, Riitta; Metsä-Kortelainen, Sini; Pinomaa, Tatu; Pajari, Heikki; Vikholm-Lundin, Inger; Pursula, Pekka; Hytönen, Vesa.

In: ACS Applied Materials & Interfaces, Vol. 9, No. 26, 2017, p. 21959-21970.

Research output: Contribution to journalArticleScientificpeer-review

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AU - Lahtinen, Panu

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AU - Metsä-Kortelainen, Sini

AU - Pinomaa, Tatu

AU - Pajari, Heikki

AU - Vikholm-Lundin, Inger

AU - Pursula, Pekka

AU - Hytönen, Vesa

PY - 2017

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N2 - We describe herein a nanocellulose-alginate hydrogel suitable for 3D printing. The composition of the hydrogel was optimized based on material characterization methods and 3D printing experiments, and its behavior during the printing process was studied using computational fluid dynamics simulations. The hydrogel was biofunctionalized by the covalent coupling of an enhanced avidin protein to the cellulose nanofibrils. Ionic cross-linking of the hydrogel using calcium ions improved the performance of the material. The resulting hydrogel is suitable for 3D printing, its mechanical properties indicate good tissue compatibility, and the hydrogel absorbs water in moist conditions, suggesting potential in applications such as wound dressings. The biofunctionalization potential was shown by attaching a biotinylated fluorescent protein and a biotinylated fluorescent small molecule via avidin and monitoring the material using confocal microscopy. The 3D-printable bioactivated nanocellulose-alginate hydrogel offers a platform for the development of biomedical devices, wearable sensors, and drug-releasing materials.

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