4-O-Methyl-beta-L-idopyranosyluronic acid linked to xylan from kraft pulp: Isolation procedure and characterisation by NMR spectroscopy

Anita Teleman (Corresponding Author), Matti Siika-aho, Harri Sorsa, Johanna Buchert, Marjukka Perttula, Tiina Hausalo, Maija Tenkanen

Research output: Contribution to journalArticleScientificpeer-review

20 Citations (Scopus)

Abstract

The tetrasaccharide 2"-0-(4-0-methyl-β-L-idopyranosyluronic acid) was isolated from enzymatically hydrolysed, unbleached, birch kraft pulp by anion-exchange chromatography in two steps. The primary structure of the tetrasaccharide was determined by 1H and 13C NMR spectroscopy, using homonuclear and heteronuclear two-dimensional techniques. NOE data and 3JH.H coupling constants show that the 4-0-methyl-β-L-idopyranosyluronic acid in the tetrasaccharide is predominantly in the 1C4 chair conformation. The pKa value (3.17) for 4-O-methyliduronic acid attached β-(1 → 2) to xylose was determined from the pH-dependent chemical shift of H-5. The amount of 4-O-methyliduronic acid (0.1–0.5 mol%) in surface xylan of unbleached birch and pine kraft pulps was determined by extensive xylanase treatment and further analysis by NMR spectroscopy and high-performance anion-exchange chromatography.
Original languageEnglish
Pages (from-to)1-13
Number of pages13
JournalCarbohydrate Research
Volume293
Issue number1
DOIs
Publication statusPublished - 1996
MoE publication typeA1 Journal article-refereed

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