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2019

Cloning of novel bacterial xylanases from lignocellulose-enriched compost metagenomic libraries

Ellilä, S., Bromann, P., Nyyssönen, M., Itävaara, M., Koivula, A., Paulin, L. & Kruus, K., 2019, In : AMB Express. 9, 1, 124.

Research output: Contribution to journalArticleScientificpeer-review

Open Access
Metagenomics
Organism Cloning
Soil
Hydrolysis
Enzymes

Effect of cellulase family and structure on modification of wood fibres at high consistency

Rahikainen, J., Ceccherini, S., Molinier, M., Holopainen-Mantila, U., Reza, M., Väisänen, S., Puranen, T., Kruus, K., Vuorinen, T., Maloney, T., Suurnäkki, A. & Grönqvist, S., 30 May 2019, In : Cellulose. 26, 8, p. 5085-5103 19 p.

Research output: Contribution to journalArticleScientificpeer-review

Open Access
Cellulase
Wood
Fibers
Pulp
Enzymes
3 Citations (Scopus)

Effect of hydrothermal pretreatment severity on lignin inhibition in enzymatic hydrolysis

Kellock, M., Maaheimo, H., Marjamaa, K., Rahikainen, J., Zhang, H., Holopainen-Mantila, U., Ralph, J., Tamminen, T., Felby, C. & Kruus, K., 2019, In : Bioresource Technology. 280, p. 303-312 10 p.

Research output: Contribution to journalArticleScientificpeer-review

Enzyme inhibition
Enzymatic hydrolysis
Lignin
lignin
hydrolysis

The liquid fraction from hydrothermal pretreatment of wheat straw provides lytic polysaccharide monooxygenases with both electrons and H2O2 co-substrate

Kont, R., Pihlajaniemi, V., Borisova, A. S., Aro, N., Marjamaa, K., Loogen, J., Büchs, J., Eijsink, V. G. H., Kruus, K. & Väljamaë, P., 8 Oct 2019, In : Biotechnology for Biofuels. 12, 1, 235.

Research output: Contribution to journalArticleScientificpeer-review

Open Access
Straw
Polysaccharides
Mixed Function Oxygenases
polysaccharide
straw
2018
2 Citations (Scopus)

A new crystal form of Aspergillus oryzae catechol oxidase and evaluation of copper site structures in coupled binuclear copper enzymes

Penttinen, L., Rutanen, C., Saloheimo, M., Kruus, K., Rouvinen, J. & Hakulinen, N., 1 May 2018, In : PLoS ONE. 13, 5, e0196691.

Research output: Contribution to journalArticleScientificpeer-review

Open Access
Aspergillus oryzae
Catechol Oxidase
Aspergillus
catechol oxidase
crystals

Bits and biomass: A roadmap to the digitalisation-empowered bioeconomy

Leinonen, A. (ed.), Åkerman, M. (ed.), Kruus, K. (ed.), Asikainen, A. (ed.), Muhonen, T. (ed.), Kohl, J. (ed.), Ala-Ilomäki, J., Arvas, M., Backman, J., Hantula, J., Häme, T., Isto, P., Kangas, A., Lantto, R., Nieminen, K., Nordlund, E., Pastell, M., Peltola, R., Pesonen, L., Pitkänen, J-P. & 7 others, Piri, T., Pusenius, J., Seisto, A., Suomi, P., Utriainen, M., Viiri, H. & Väätäinen, K., 2018, VTT Technical Research Centre of Finland. 51 p. (VTT Visions; No. 12).

Research output: Book/ReportReport

Open Access
Natural resources
Biomass
Raw materials
Production control
Transparency
1 Citation (Scopus)

Cellulases adsorb reversibly on biomass lignin

Djajadi, D. T., Pihlajaniemi, V., Rahikainen, J., Kruus, K. & Meyer, A. S., 21 Aug 2018, In : Biotechnology and Bioengineering. 115, 12, p. 2869-2880 12 p.

Research output: Contribution to journalArticleScientificpeer-review

Open Access
Cellulases
Lignin
Biomass
Adsorption
Straw
1 Citation (Scopus)
Xylans
Peeling
Fibers
Chemical analysis
Hydrolysis
3 Citations (Scopus)

Enzyme biotechnology in degradation and modification of plant cell wall polymers

Marjamaa, K. & Kruus, K., 1 Sep 2018, In : Physiologia Plantarum. 164, 1, p. 106-118 13 p.

Research output: Contribution to journalArticleScientificpeer-review

Plant Cells
Biotechnology
Cell Wall
biotechnology
polymers
5 Citations (Scopus)

Impact of thermochemical pre-treatment and carbohydrate and protein hydrolyzing enzyme treatment on fractionation of protein and lignin from brewer's spent grain

Rommi, K., Niemi, P., Kemppainen, K. & Kruus, K., 1 Jan 2018, In : Journal of Cereal Science. 79, p. 168-173 6 p.

Research output: Contribution to journalArticleScientificpeer-review

spent grains
brewers grains
Lignin
enzymatic treatment
Fractionation

Screening of glycoside hydrolases and ionic liquids for fibre modification

Rahikainen, J., Anbarasan, S., Wahlström, R., Parviainen, A., King, A. W. T., Puranen, T., Kruus, K., Kilpeläinen, I., Turunen, O. & Suurnäkki, A., 2018, In : Journal of Chemical Technology and Biotechnology. 93, 3, p. 818-826 9 p.

Research output: Contribution to journalArticleScientificpeer-review

Ionic Liquids
Glycoside Hydrolases
Ionic liquids
Screening
Fibers
2017
4 Citations (Scopus)

A novel Colletotrichum graminicola raffinose oxidase in the AA5 family

Blomster Andberg, M., Mollerup, F., Parikka, K., Koutaniemi, S., Boer, H., Juvonen, M., Master, E., Tenkanen, M. & Kruus, K., 2017, In : Applied and Environmental Microbiology. 83, 20, 17 p., e01383.

Research output: Contribution to journalArticleScientificpeer-review

Open Access
Glomerella graminicola
Colletotrichum
Raffinose
raffinose
substrate preference

Bittejä ja biomassaa: Tiekartta digitalisaation vauhdittamaan biotalouteen

Leinonen, A. (ed.), Åkerman, M. (ed.), Kruus, K. (ed.), Asikainen, A. (ed.), Muhonen, T. (ed.), Kohl, J. (ed.), Ala-Ilomäki, J., Arvas, M., Backman, J., Hantula, J., Holmala, K., Häme, T., Kangas, A., Isto, P., Lantto, R., Nieminen, K., Pastell, M., Nordlund, E., Peltola, R., Pesonen, L. & 8 others, Pitkänen, J-P., Piri, T., Pusenius, J., Seisto, A., Suomi, P., Utriainen, M., Viiri, H. & Väätäinen, K., 2017, VTT Technical Research Centre of Finland. 103 p. (VTT Visions; No. 11).

Research output: Book/ReportReport

Open Access
1 Citation (Scopus)

Characterization of sulfhydryl oxidase from Aspergillus tubingensis

Nivala, O., Faccio, G., Arvas, M., Permi, P., Buchert, J., Kruus, K. & Mattinen, M. L., 8 Dec 2017, In : BMC biochemistry. 18, 1, 1 p., 15.

Research output: Contribution to journalArticleScientificpeer-review

Open Access
Aspergillus
Enzymes
Glutathione
Gene encoding
Gliotoxin
24 Citations (Scopus)

Lignin-derived inhibition of monocomponent cellulases and a xylanase in the hydrolysis of lignocellulosics

Kellock, M., Rahikainen, J., Marjamaa, K. & Kruus, K., 11 Feb 2017, In : Bioresource Technology. 232, p. 183-191 9 p.

Research output: Contribution to journalArticleScientificpeer-review

Cellulases
Lignin
lignin
hydrolysis
Hydrolysis
11 Citations (Scopus)

Lignin Films from Spruce, Eucalyptus, and Wheat Straw Studied with Electroacoustic and Optical Sensors: Effect of Composition and Electrostatic Screening on Enzyme Binding

Pereira, A., Hoeger, I. C., Ferrer, A., Rencoret, J., Del Rio, J., Kruus, K., Rahikainen, J., Kellock, M., Gutiérrez, A. & Rojas, O. J., 10 Apr 2017, In : Biomacromolecules. 18, 4, p. 1322-1332 11 p.

Research output: Contribution to journalArticleScientificpeer-review

Lignin
Optical sensors
Straw
Electrostatics
Screening
10 Citations (Scopus)

Purification and concentration of lignin from the spent liquor of the alkaline oxidation of woody biomass through membrane separation technology

Servaes, K., Varhimo, A., Dubreuil, M., Bulut, M., Vandezande, P., Siika-aho, M., Sirviö, J., Kruus, K., Porto-Carrero, W. & Bongers, M., 1 Nov 2017, In : Industrial Crops and Products. 106, p. 86-96 11 p.

Research output: Contribution to journalArticleScientificpeer-review

lignin
oxidation
biomass
softwood
pretreatment
11 Citations (Scopus)

Structuring colloidal oat and faba bean protein particles via enzymatic modification

Nivala, O., Mäkinen, O., Kruus, K., Nordlund, E. & Ercili-Cura, D., 15 Sep 2017, In : Food Chemistry. 231, p. 87-95 9 p.

Research output: Contribution to journalArticleScientificpeer-review

Vicia faba
oat protein
protein-glutamine gamma-glutamyltransferase
faba beans
foaming properties
71 Downloads (Pure)

The Making of BIOECONOMY TRANSFORMATION

Kruus, K. (ed.) & Hakala, T. (ed.), 2017, VTT Technical Research Centre of Finland. 84 p.

Research output: Book/ReportReport

Open Access
File
national strategy
programme
ecosystem
research centre
2016
31 Citations (Scopus)

Back to the future of soil metagenomics

Nesme, J., Achouak, W., Agathos, S. N., Bailey, M. J., Simonet, P., Kruus, K. & et al., 2016, In : Frontiers in Microbiology. 7

Research output: Contribution to journalArticleScientificpeer-review

Open Access
10 Citations (Scopus)

Chemical characterization of hydrothermally pretreated and enzyme-digested wheat straw : an evaluation of recalcitrance

Merali, Z., Marjamaa, K., Käsper, A., Kruus, K., Gunning, A. P., Morris, V. J. & Waldron, K. W., 2016, In : Food Chemistry. 198, p. 132-140

Research output: Contribution to journalArticleScientificpeer-review

Straw
wheat straw
Triticum
Saccharification
saccharification
18 Citations (Scopus)

Comparison of three deep eutectic solvents and 1-ethyl-3-methylimidazolium acetate in the pretreatment of lignocellulose: effect on enzyme stability, lignocellulose digestibility and one-pot hydrolysis

Wahlström, R., Hiltunen, J., Pitaluga de Souza Nascente Sirkka, M., Vuoti, S. & Kruus, K., 2016, In : RSC Advances. 6, 72, p. 68100-68110

Research output: Contribution to journalArticleScientificpeer-review

Eutectics
Hydrolysis
Enzymes
Ionic Liquids
Ionic liquids

Effect of hydrothermal pretreatment severity on lignin inhibition in the enzymatic hydrolysis of lignocellulosic biomass

Kellock, M., Zhang, H., Marjamaa, K., Tamminen, T., Felby, C. & Kruus, K., 2016.

Research output: Contribution to conferenceConference articleScientific

enzymatic hydrolysis
lignin
pretreatment
biomass
enzymes

Effect of pretreatment severity on the enzymatic hydrolysis of spruce and wheat straw

Kellock, M., Zhang, H., Marjamaa, K., Tamminen, T., Felby, C. & Kruus, K., 2016.

Research output: Contribution to conferenceConference articleScientific

Effect of pretreatment severity on the inhibitory nature of lignin in the enzymatic hydrolysis of lignocellulose

Kellock, M., Zhang, H., Marjamaa, K., Tamminen, T., Felby, C. & Kruus, K., 2016.

Research output: Contribution to conferenceConference articleScientific

11 Citations (Scopus)

Steam explosion of Brewer’s spent grain improves enzymatic digestibility of carbohydrates and affects solubility and stability of proteins

Kemppainen, K., Rommi, K., Holopainen, U. & Kruus, K., 15 Apr 2016, In : Applied Biochemistry and Biotechnology. 180, 1, p. 94-108

Research output: Contribution to journalArticleScientificpeer-review

Explosions
Protein Stability
Steam
Carbohydrates
Solubility
2015
5 Citations (Scopus)

Treatment of milk fat with sn-2 specific Pseudozyma antarctica lipase A for targeted hydrolysis of saturated medium and long-chain fatty acids

Nyyssölä, A., Miettinen, H., Kontkanen, H., Lille, M., Partanen, R., Rokka, S., Järvenpää, E., Lantto, R. & Kruus, K., 2015, In : International Dairy Journal. 41, p. 16-22

Research output: Contribution to journalArticleScientificpeer-review

Pseudozyma antarctica
medium chain fatty acids
long chain fatty acids
Lipase
milk fat
2014
19 Citations (Scopus)

Cellulose hydrolysis and binding with Trichoderma reesei Cel5A and Cel7A and their core domains in ionic liquid solutions

Wahlström, R., Rahikainen, J., Kruus, K. & Suurnäkki, A., 2014, In : Biotechnology and Bioengineering. 111, 4, p. 726-733

Research output: Contribution to journalArticleScientificpeer-review

Ionic Liquids
Trichoderma
Ionic liquids
Cellulose
Hydrolysis

Effect of lignin chemistry on non-productive enzyme adsorption in the enzymatic hydrolysis of lignocellulose

Rahikainen, J., Martin-Sampedro, R., Heikkinen, H., Rovio, S., Marjamaa, K., Tamminen, T., Rojas, O. J. & Kruus, K., 2014, Proceedings of the 13th European Workshop on Lignocellulosics and Pulp: EWLP 2014. Río Andrade, J. C. D., Gutiérrez Suárez, A., Rencoret, J. & Martínez, Á. T. (eds.). Sevilla, p. 49-52 O.13

Research output: Chapter in Book/Report/Conference proceedingConference article in proceedingsScientificpeer-review

Open Access
lignocellulose
enzymatic hydrolysis
lignin
adsorption
chemistry
3 Citations (Scopus)

Hydrolysis and composition of recovered fibres fractionated from solid recovered fuel

Kemppainen, K., Siika-aho, M., Östman, A., Sipilä, E., Puranen, T., von Weymarn, N. & Kruus, K., 2014, In : Bioresource Technology. 169, p. 88-95 8 p.

Research output: Contribution to journalArticleScientificpeer-review

hydrolysis
Hydrolysis
Fibers
Chemical analysis
Carbohydrates
28 Citations (Scopus)

Impact of steam explosion on the wheat straw lignin structure studied by solution-state nuclear magnetic resonance and density functional methods

Heikkinen, H., Elder, T., Maaheimo, H., Rovio, S., Rahikainen, J., Kruus, K. & Tamminen, T., 2014, In : Journal of Agricultural and Food Chemistry. 62, 43, p. 10437-10444 7 p.

Research output: Contribution to journalArticleScientificpeer-review

Explosions
Lignin
explosions
Steam
Straw
45 Citations (Scopus)

Spruce bark as an industrial source of condensed tannins and non-cellulosic sugars

Kemppainen, K., Siika-aho, M., Pattathi, S., Giovando, S. & Kruus, K., 2014, In : Industrial Crops and Products. 52, p. 158-168 11 p.

Research output: Contribution to journalArticleScientificpeer-review

proanthocyanidins
Picea
bark
sugars
tannins
2013
84 Citations (Scopus)

Cellulase-lignin interactions: The role of carbohydrate-binding module and pH in non-productive binding

Rahikainen, J., Evans, J. D., Mikander, S., Kalliola, A., Puranen, T., Tamminen, T., Marjamaa, K. & Kruus, K., 2013, In : Enzyme and Microbial Technology. 53, 5, p. 315-321 6 p.

Research output: Contribution to journalArticleScientificpeer-review

Cellulase
Lignin
Carbohydrates
Enzymes
Coulomb interactions
16 Citations (Scopus)

Cellulose hydrolysis with thermo- and alkali-tolerant cellulases in cellulose-dissolving superbase ionic liquids

Wahlström, R., King, A., Parviainen, A., Kruus, K. & Suurnäkki, A., 2013, In : RSC Advances. 3, 43, p. 20001-20009 8 p.

Research output: Contribution to journalArticleScientificpeer-review

Cellulases
Ionic Liquids
Alkalies
Ionic liquids
Cellulose
2 Citations (Scopus)

Discovery of novel secreted fungal sulfhydryl oxidases with a plate test screen

Nivala, O., Mattinen, M., Faccio, G., Buchert, J. & Kruus, K., 2013, In : Applied Microbiology and Biotechnology. 97, 21, p. 9429-9437 9 p.

Research output: Contribution to journalArticleScientificpeer-review

Penicillium
Chaetomium
Trametes
Nitrobenzoates
Dithionitrobenzoic Acid
30 Citations (Scopus)

Effect of temperature on lignin-derived inhibition studied with three structurally different cellobiohydrolases

Rahikainen, J., Moilanen, U., Nurmi-Rantala, S., Lappas, A., Koivula, A., Viikari, L. & Kruus, K., 2013, In : Bioresource Technology. 146, p. 118-125 7 p.

Research output: Contribution to journalArticleScientificpeer-review

Cellulose 1,4-beta-Cellobiosidase
Lignin
lignin
Enzymes
enzyme
20 Citations (Scopus)

Hydroperoxide production from linoleic acid by heterologous Gaeumannomyces graminis tritici lipoxygenase: Optimization and scale-up

Villaverde, J. J., van der Vlist, V., Santos, S. A. O., Haarmann, T., Langfelder, K., Pirttimaa, M., Nyyssölä, A., Jylhä, S., Tamminen, T., Kruus, K., de Graaff, L., Neto, C. P., Simoes, M. M. Q., Domingues, M. R. M., Silvestre, A. J. D., Eidner, J. & Buchert, J., 2013, In : Chemical Engineering Journal. 217, p. 82-90 9 p.

Research output: Contribution to journalArticleScientificpeer-review

Linoleic acid
Lipoxygenase
Linoleic Acid
Hydrogen Peroxide
Acids
43 Citations (Scopus)

Impact of hydrothermal pre-treatment to chemical composition, enzymatic digestibility and spatial distribution of cell wall polymers

Holopainen-Mantila, U., Marjamaa, K., Merali, Z., Käsper, A., de Bot, P., Jääskeläinen, A. S., Waldron, K., Kruus, K. & Tamminen, T., 2013, In : Bioresource Technology. 138, p. 156-162 7 p.

Research output: Contribution to journalArticleScientificpeer-review

digestibility
Cellulose
Spatial distribution
cellulose
Polymers
196 Citations (Scopus)

Inhibitory effect of lignin during cellulose bioconversion: The effect of lignin chemistry on non-productive enzyme adsorption

Rahikainen, J., Martin-Sampedro, R., Heikkinen, H., Rovio, S., Marjamaa, K., Tamminen, T., Rojas, O. J. & Kruus, K., 2013, In : Bioresource Technology. 133, p. 270-278 9 p.

Research output: Contribution to journalArticleScientificpeer-review

Bioconversion
Lignin
Cellulose
lignin
cellulose
25 Citations (Scopus)

Novel Penicillium cellulases for total hydrolysis of lignocellulosics

Marjamaa, K., Toth, K., Bromann, P. A., Szakacs, G. & Kruus, K., 2013, In : Enzyme and Microbial Technology. 52, 6-7, p. 358-369 12 p.

Research output: Contribution to journalArticleScientificpeer-review

Cellulases
Lignin
Penicillium
Hydrolysis
Cellulose
11 Citations (Scopus)

On the factors affecting product distribution in laccase-catalyzed oxidation of a lignin model compound vanillyl alcohol: Experimental and computational evaluation

Lahtinen, M., Heinonen, P., Oivanen, M., Karhunen, P., Kruus, K. & Sipilä, J., 2013, In : Organic and Biomolecular Chemistry. 11, 33, p. 5454-5464 10 p.

Research output: Contribution to journalArticleScientificpeer-review

Open Access
Laccase
lignin
Lignin
alcohols
Oxidation
11 Citations (Scopus)

On the reactivity of the Melanocarpus albomyces laccase and formation of coniferyl alcohol dehydropolymer (DHP) in the presence of ionic liquid 1-allyl-3-methylimidazolium chloride

Lahtinen, M., Viikari, L., Karhunen, P., Asikkala, J., Kruus, K. & Kilpeläinen, I., 2013, In : Journal of Molecular Catalysis B: Enzymatic. 85-86, p. 169-177 9 p.

Research output: Contribution to journalArticleScientificpeer-review

Methyl Chloride
Ionic Liquids
Laccase
Ionic liquids
Chlorides
1 Citation (Scopus)

Polysaccharide-acting enzymes and their applications

Koivula, A., Voutilainen, S., Pere, J., Kruus, K., Suurnäkki, A., van der Broek, L. A. M., Bakker, R. & Lips, S., 2013, The European Polysaccharide Network of Excellence (EPNOE): Research initiative and results. Navard, P. (ed.). Springer, p. 375-392

Research output: Chapter in Book/Report/Conference proceedingChapter or book articleProfessional

54 Citations (Scopus)

Preferential adsorption and activity of monocomponent cellulases on lignocellulose thin films with varying lignin content

Martin-Sampedro, R., Rahikainen, J., Johansson, L-S., Marjamaa, K., Laine, J., Kruus, K. & Rojas, O. J., 2013, In : Biomacromolecules. 14, 4, p. 1231-1239 9 p.

Research output: Contribution to journalArticleScientificpeer-review

Cellulases
Lignin
Cellulose
Adsorption
Thin films
5 Citations (Scopus)

Screening of microbes for lipases specific for saturated medium and long-chain fatty acids of milk fat

Miettinen, H., Nyyssölä, A., Rokka, S., Kontkanen, H. & Kruus, K., 2013, In : International Dairy Journal. 32, 2, p. 61-67 6 p.

Research output: Contribution to journalArticleScientificpeer-review

medium chain fatty acids
long chain fatty acids
Lipase
milk fat
saturated fatty acids
24 Citations (Scopus)

Screening of microbes for novel acidic cutinases and cloning and expression of an acidic cutinase from Aspergillus niger CBS 513.88

Nyyssölä, A., Pihlajaniemi, V., Järvinen, R., Mikander, S., Kontkanen, H., Kruus, K., Kallio, H. & Buchert, J., 2013, In : Enzyme and Microbial Technology. 52, 4-5, p. 272-278 7 p.

Research output: Contribution to journalArticleScientificpeer-review

Aspergillus niger
Cloning
Aspergillus
Organism Cloning
Hydrolysis

Spruce bark biorefinery

Kemppainen, K., Niemelä, T., Inkinen, J., Uusitalo, J., Kaijaluoto, S., Sorsamäki, L., Aaltonen, O., Suurnäkki, A., Peltonen, S., Mikkonen, H., Grönberg, V., Kruus, K., Nakari-Setälä, T. & Siika-aho, M., 2013, Proceedings of the 21st European Biomass Conference. p. 540-543

Research output: Chapter in Book/Report/Conference proceedingConference article in proceedingsScientific

biorefining
Picea
bark
tannins
forest industries
25 Citations (Scopus)

The crystal structure of an extracellular catechol oxidase from the ascomycete fungus Aspergillus oryzae

Hakulinen, N., Gasparetti, C., Kaljunen, H., Kruus, K. & Rouvinen, J., 2013, In : Journal of Biological Inorganic Chemistry. 18, 8, p. 917-929 12 p.

Research output: Contribution to journalArticleScientificpeer-review

Aspergillus oryzae
Catechol Oxidase
Ascomycota
Monophenol Monooxygenase
Aspergillus
51 Citations (Scopus)

Topochemical pretreatment of wood biomass to enhance enzymatic hydrolysis of polysaccharides to sugars

Mou, H-Y., Orblin, E., Kruus, K. & Fardim, P., 2013, In : Bioresource Technology. 142, p. 540-545 5 p.

Research output: Contribution to journalArticleScientificpeer-review

Enzymatic hydrolysis
Lignin
Polysaccharides
polysaccharide
Sugars
2012
48 Citations (Scopus)

Bacterial tyrosinases and their applications

Faccio, G., Kruus, K., Saloheimo, M. & Thöny-Meyer, L., 2012, In : Process Biochemistry. 47, 12, p. 1749-1760 11 p.

Research output: Contribution to journalReview ArticleScientificpeer-review

Monophenol Monooxygenase
Substrates
Melanin
Food processing
Nucleophiles