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2019

Bioconjugation with Aminoalkylhydrazine for Efficient Mass Spectrometry-Based Detection of Small Carbonyl Compounds

Thangaraj, S. K., Voutilainen, S., Andberg, M., Koivula, A., Jänis, J. & Rouvinen, J., 20 Aug 2019, In : ACS Omega. 4, 8, p. 13447 - 13453 7 p.

Research output: Contribution to journalArticleScientificpeer-review

Open Access
Carbonyl compounds
Deoxyribose
Mass spectrometry
Hydrazones
Electrospray ionization

Cloning of novel bacterial xylanases from lignocellulose-enriched compost metagenomic libraries

Ellilä, S., Bromann, P., Nyyssönen, M., Itävaara, M., Koivula, A., Paulin, L. & Kruus, K., 2019, In : AMB Express. 9, 1, 124.

Research output: Contribution to journalArticleScientificpeer-review

Open Access
Metagenomics
Organism Cloning
Soil
Hydrolysis
Enzymes

In vitro reconstitution and characterisation of the oxidative d-xylose pathway for production of organic acids and alcohols

Boer, H., Andberg, M., Pylkkänen, R., Maaheimo, H. & Koivula, A., 2019, In : AMB Express. 9, 1, 48.

Research output: Contribution to journalArticleScientificpeer-review

Open Access
Xylose
glycolic acid
Alcohols
Acids
Enzymes
2018
20 Citations (Scopus)

A glucose/oxygen enzymatic fuel cell based on gold nanoparticles modified graphene screen-printed electrode. Proof-of-concept in human saliva

Bollella, P., Fusco, G., Stevar, D., Gorton, L., Ludwig, R., Ma, S., Boer, H., Koivula, A., Tortolini, C., Favero, G., Antiochia, R. & Mazzei, F., 2018, In : Sensors and Actuators B: Chemical. 256, p. 921-930 10 p.

Research output: Contribution to journalArticleScientificpeer-review

Enzymatic fuel cells
saliva
Graphite
glucose
Gold
4 Citations (Scopus)

The crystal structure of D-xylonate dehydratase reveals functional features of enzymes from the Ilv/ED dehydratase family

Rahman, M. M., Andberg, M., Koivula, A., Rouvinen, J. & Hakulinen, N., 1 Dec 2018, In : Scientific Reports. 8, 1, 865.

Research output: Contribution to journalArticleScientificpeer-review

Open Access
Hydro-Lyases
Monomers
Crystal structure
Enzymes
Catalysis
2017
7 Citations (Scopus)

The Crystal Structure of a Bacterial l -Arabinonate Dehydratase Contains a [2Fe-2S] Cluster

Rahman, M., Andberg, M., Thangaraj, S., Parkkinen, T., Penttilä, M., Jänis, J., Koivula, A., Rouvinen, J. & Hakulinen, N., 21 Jul 2017, In : ACS Chemical Biology. 12, 7, p. 1919-1927 9 p.

Research output: Contribution to journalArticleScientificpeer-review

Hydro-Lyases
Bacterial Structures
Dimers
Catalytic Domain
Crystal structure
2016
14 Citations (Scopus)

Characterization and mutagenesis of two novel iron-sulphur cluster pentonate dehydratases

Andberg, M., Aro-Kärkkäinen, N., Carlson, P., Oja, M., Bozonnet, S., Toivari, M., Hakulinen, N., O'Donohue, M., Penttilä, M. & Koivula, A., 2016, In : Applied Microbiology and Biotechnology. 100, 17, p. 7549-7563

Research output: Contribution to journalArticleScientificpeer-review

Hydro-Lyases
Sulfur
Mutagenesis
Iron
Sugar Acids
2 Citations (Scopus)

Characterization of a unique Caulobacter crescentus aldose-aldose oxidoreductase having dual activities

Andberg, M., Maaheimo, H., Kumpula, E-P., Boer, H., Toivari, M., Penttilä, M. & Koivula, A., 2016, In : Applied Microbiology and Biotechnology. 100, 2, p. 673-685

Research output: Contribution to journalArticleScientificpeer-review

Caulobacter crescentus
Oxidoreductases
Arabinose
Monosaccharides
Xylose
3 Citations (Scopus)
Open Access
Caulobacter crescentus
Rhizobium leguminosarum
Hydro-Lyases
formates
Crystallization
7 Citations (Scopus)

Expression of Two Novel β-Glucosidases from Chaetomium atrobrunneum in Trichoderma reesei and Characterization of the Heterologous Protein Products

Colabardini, A. C., Valkonen, M., Huuskonen, A., Siika-aho, M., Koivula, A., Goldman, G. H. & Saloheimo, M., 1 Dec 2016, In : Molecular Biotechnology. 58, 12, p. 821-831 11 p.

Research output: Contribution to journalArticleScientificpeer-review

Chaetomium
Glucosidases
Trichoderma
Enzymes
Proteins
15 Citations (Scopus)

Production and applications of carbohydrate-derived sugar acids as generic biobased chemicals

Mehtiö, T., Toivari, M., Wiebe, M. G., Harlin, A., Penttilä, M. & Koivula, A., 2016, In : Critical Reviews in Biotechnology. 36, 5, p. 904-916

Research output: Contribution to journalArticleScientificpeer-review

Sugar Acids
Carbohydrates
Sequestering Agents
Uronic Acids
Hydrogels
2015
8 Citations (Scopus)

A novel aldose-aldose oxidoreductase for co-production of D-xylonate and xylitol from D-xylose with Saccharomyces cerevisiae

Wiebe, M. G., Nygård, Y., Oja, M., Andberg, M., Ruohonen, L., Koivula, A., Penttilä, M. & Toivari, M., 2015, In : Applied Microbiology and Biotechnology. 99, 22, p. 9439-9447

Research output: Contribution to journalArticleScientificpeer-review

Open Access
Xylitol
Xylose
Saccharomyces cerevisiae
Caulobacter crescentus
Oxidoreductases
3 Citations (Scopus)

Structure and function of Caulobacter crescentus aldose-aldose oxidoreductase

Taberman, H., Andberg, M., Koivula, A., Hakulinen, N., Penttilä, M., Rouvinen, J. & Parkkinen, T., 2015, In : Biochemical Journal. 472, 3, p. 297-307

Research output: Contribution to journalArticleScientificpeer-review

2014
21 Citations (Scopus)

Engineering chimeric thermostable GH7 cellobiohydrolases in Saccharomyces cerevisiae

Voutilainen, S. P., Nurmi-Rantala, S., Penttilä, M. & Koivula, A., 1 Jan 2014, In : Applied Microbiology and Biotechnology. 98, 7, p. 2991-3001

Research output: Contribution to journalArticleScientificpeer-review

Cellulose 1,4-beta-Cellobiosidase
Saccharomyces cerevisiae
Hydrolysis
Enzymes
Cellulose
6 Citations (Scopus)

l-Arabinose/d-galactose 1-dehydrogenase of Rhizobium leguminosarum bv. trifolii characterised and applied for bioconversion of l-arabinose to l-arabonate with Saccharomyces cerevisiae

Aro-Kärkkäinen, N., Toivari, M., Maaheimo, H., Ylilauri, M., Pentikäinen, O. T., Andberg, M., Oja, M., Penttilä, M., Wiebe, M. G., Ruohonen, L. & Koivula, A., 1 Jan 2014, In : Applied Microbiology and Biotechnology. 98, 23, p. 9653-9665 13 p.

Research output: Contribution to journalArticleScientificpeer-review

galactose dehydrogenase
Rhizobium leguminosarum
Arabinose
Saccharomyces cerevisiae
Yeasts
5 Citations (Scopus)

Purification, crystallization and preliminary X-ray diffraction analysis of a novel keto-deoxy-D-galactarate (KDG) dehydratase from Agrobacterium tumefaciens

Taberman, H., Andberg, M., Parkkinen, T., Richard, P., Hakulinen, N., Koivula, A. & Rouvinen, J., 2014, In : Acta Crystallographica Section F: Structural Biology Communications. 70, p. 49-52 4 p.

Research output: Contribution to journalArticleScientificpeer-review

Hydro-Lyases
Agrobacterium tumefaciens
Crystallization
purification
X-Ray Diffraction
23 Citations (Scopus)

Single-molecule imaging analysis of elementary reaction steps of Trichoderma reesei cellobiohydrolase I (Cel7A) hydrolyzing crystalline cellulose Ia and IIII

Shibafuji, Y., Nakamura, A., Uchihashi, T., Sugimoto, N., Fukuda, S., Watanabe, H., Samejima, M., Ando, T., Noji, H., Koivula, A., Igarashi, K. & Iino, R., 2014, In : Journal of Biological Chemistry. 289, 20, p. 14056-14065 10 p.

Research output: Contribution to journalArticleScientificpeer-review

Cellulose 1,4-beta-Cellobiosidase
Trichoderma
Cellulose
Crystalline materials
Imaging techniques
7 Citations (Scopus)

Structure and function of a decarboxylating Agrobacterium tumefaciens Keto-deoxy-D-galactarate dehydratase

Taberman, H., Andberg, M., Parkkinen, T., Jänis, J., Penttilä, M., Hakulinen, N., Koivula, A. & Rouvinen, J., 30 Dec 2014, In : Biochemistry. 53, 51, p. 8052-8060 9 p.

Research output: Contribution to journalArticleScientificpeer-review

Hydro-Lyases
Agrobacterium tumefaciens
uronate dehydrogenase
Genes
Crystal structure
2013
29 Citations (Scopus)

Effect of temperature on lignin-derived inhibition studied with three structurally different cellobiohydrolases

Rahikainen, J., Moilanen, U., Nurmi-Rantala, S., Lappas, A., Koivula, A., Viikari, L. & Kruus, K., 2013, In : Bioresource Technology. 146, p. 118-125 7 p.

Research output: Contribution to journalArticleScientificpeer-review

Cellulose 1,4-beta-Cellobiosidase
Lignin
lignin
Enzymes
enzyme

Identification and characterization of enzymes involved in the oxidative D-galacturonic acid pathway

Koivula, A., Andberg, M., Maaheimo, H., Boer, H., Mojzita, D., Wiebe, M., Richard, P., Parkkinen, T., Taberman, H., Rouvinen, J. & Penttilä, M., 2013.

Research output: Contribution to conferenceConference AbstractScientific

1 Citation (Scopus)

Polysaccharide-acting enzymes and their applications

Koivula, A., Voutilainen, S., Pere, J., Kruus, K., Suurnäkki, A., van der Broek, L. A. M., Bakker, R. & Lips, S., 2013, The European Polysaccharide Network of Excellence (EPNOE): Research initiative and results. Navard, P. (ed.). Springer, p. 375-392

Research output: Chapter in Book/Report/Conference proceedingChapter or book articleProfessional

49 Citations (Scopus)

The Tryptophan Residue at the Active Site Tunnel Entrance of Trichoderma reesei Cellobiohydrolase Cel7A Is Important for Initiation of Degradation of Crystalline Cellulose

Nakamura, A., Tsukada, T., Auer, S., Furuta, T., Wada, M., Koivula, A., Igarashi, K. & Samejima, M., 2013, In : Journal of Biological Chemistry. 288, 19, p. 13503-13510 8 p.

Research output: Contribution to journalArticleScientificpeer-review

Cellulose 1,4-beta-Cellobiosidase
Trichoderma
Cellulose
Tryptophan
Catalytic Domain
2012
24 Citations (Scopus)
Agrobacterium tumefaciens
uronate dehydrogenase
Lactones
mandelate racemase
Enzymes
24 Citations (Scopus)

Engineering chitinases for the synthesis of chitin oligosaccharides: Catalytic amino acid mutations convert the GH-18 family glycoside hydrolases into transglycosylases

Martinez, E. A., Boer, H., Koivula, A., Samain, E., Driguez, H., Armand, S. & Cottaz, S., 2012, In : Journal of Molecular Catalysis B: Enzymatic. 74, 1-2, p. 89-96 8 p.

Research output: Contribution to journalArticleScientificpeer-review

Chitinases
Chitin
Oligosaccharides
Glycoside Hydrolases
Amino acids

Lignin-derived inhibition of GH7 cellobiohydrolases: Insight to the inhibitory mechanism

Rahikainen, J., Marjamaa, K., Nurmi-Rantala, S., Koivula, A. & Kruus, K., 2012.

Research output: Contribution to conferenceConference articleScientific

152 Citations (Scopus)

Lignocellulosic ethanol: From science to industry

Viikari, L., Vehmaanperä, J. & Koivula, A., 2012, In : Biomass and Bioenergy. 46, p. 13-24 11 p.

Research output: Contribution to journalArticleScientificpeer-review

ethanol
Ethanol
industry
ethanol production
Enzymes
44 Citations (Scopus)

Metabolic engineering of Saccharomyces cerevisiae for bioconversion of d-xylose to d-xylonate

Toivari, M., Nygård, Y., Kumpula, E. P., Vehkomäki, M. L., Benčina, M., Valkonen, M., Maaheimo, H., Andberg, M., Koivula, A., Ruohonen, L., Penttilä, M. & Wiebe, M. G., 1 Jul 2012, In : Metabolic Engineering. 14, 4, p. 427-436

Research output: Contribution to journalArticleScientificpeer-review

Open Access
Metabolic engineering
Metabolic Engineering
Bioconversion
Xylose
Xylitol
11 Citations (Scopus)

Visualization of cellobiohydrolase i from trichoderma reesei moving on crystalline cellulose using high-speed atomic force microscopy

Igarashi, K., Uchihashi, T., Koivula, A., Wada, M., Kimura, S., Penttilä, M., Ando, T. & Samejima, M., 22 May 2012, In : Methods in Enzymology. 510, p. 169-182

Research output: Contribution to journalArticleScientificpeer-review

Cellulose 1,4-beta-Cellobiosidase
Trichoderma
Atomic Force Microscopy
Cellulose
Atomic force microscopy
2011
43 Citations (Scopus)

Crystal structure of an ascomycete fungal laccase from Thielavia arenaria : Common structural features of asco-laccases

Kallio, J. P., Gasparetti, C., Andberg, M., Boer, H., Koivula, A., Kruus, K., Rouvinen, J. & Hakulinen, N., 2011, In : FEBS Journal. 278, 13, p. 2283-2295 14 p.

Research output: Contribution to journalArticleScientificpeer-review

Open Access
Fungal Structures
Laccase
Ascomycota
Crystal structure
Proton transfer
24 Citations (Scopus)

Crystal structure of uronate dehydrogenase from Agrobacterium tumefaciens

Parkkinen, T., Boer, H., Jänis, J., Andberg, M., Penttilä, M., Koivula, A. & Rouvinen, J., 5 Aug 2011, In : Journal of Biological Chemistry. 286, 31, p. 27294-27300

Research output: Contribution to journalArticleScientificpeer-review

uronate dehydrogenase
Agrobacterium tumefaciens
NAD
Oxidoreductases
Crystal structure
97 Citations (Scopus)

High level secretion of cellobiohydrolases by Saccharomyces cerevisiae

Ilmén, M., Den Haan, R., Brevnova, E., McBride, J., Wiswall, E., Froehlich, A., Koivula, A., Voutilainen, S. P., Siika-Aho, M., La Grange, D. C., Thorngren, N., Ahlgren, S., Mellon, M., Deleault, K., Rajgarhia, V., Van Zyl, W. H. & Penttilä, M., 14 Sep 2011, In : Biotechnology for Biofuels. 4, 30.

Research output: Contribution to journalArticleScientificpeer-review

Open Access
Cellulose 1,4-beta-Cellobiosidase
secretion
Yeast
Saccharomyces cerevisiae
cellulose
333 Citations (Scopus)

Traffic jams reduce hydrolytic efficiency of cellulase on cellulose surface

Igarashi, K., Uchihashi, T., Koivula, A., Wada, M., Kimura, S., Okamoto, T., Penttilä, M., Ando, T. & Samejima, M., 2 Sep 2011, In : Science. 333, 6047, p. 1279-1282

Research output: Contribution to journalArticleScientificpeer-review

Cellulase
Cellulose
Cellulose 1,4-beta-Cellobiosidase
Enzymes
Trichoderma
2010

Biofuel cell based on printed bioelectrocatalytic layers

Smolander, M., Vaari, A., Tuurala, S., Valkiainen, M., Boer, H., Koivula, A., Keskinen, J., Kaukoniemi, O-V., Uotila, J. & Bergelin, M., 2010, 217th ECS Meeting Abstracts 2010. Curran Associates Inc., Vol. 1. p. 459

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsScientific

26 Citations (Scopus)

Electrochemical evaluation of electron transfer kinetics of high and low redox potential laccases on gold electrode surface

Frasconi, M., Boer, H., Koivula, A. & Mazzei, F., 2010, In : Electrochimica Acta. 56, 2, p. 817-827 11 p.

Research output: Contribution to journalArticleScientificpeer-review

Laccase
Gold
Electrodes
Kinetics
Electrons

Exploration of the phenolic substrate binding site and the C-terminus of Melanocarpus albomyces laccase by site-directed mutagenesis

Andberg, M., Hakulinen, N., Auer, S., Koivula, A., Rouvinen, J. & Kruus, K., 2010.

Research output: Contribution to conferenceConference articleScientific

82 Citations (Scopus)

Expression of Talaromyces emersonii cellobiohydrolase Cel7A in Saccharomyces cerevisiae and rational mutagenesis to improve its thermostability and activity

Voutilainen, S., Murray, P. G., Tuohy, M. G. & Koivula, A., 2010, In : Protein Engineering, Design and Selection. 23, 2, p. 69-79 11 p.

Research output: Contribution to journalArticleScientificpeer-review

Talaromyces
Cellulose 1,4-beta-Cellobiosidase
Mutagenesis
Yeast
Saccharomyces cerevisiae
35 Citations (Scopus)

Identification in Agrobacterium tumefaciens of the D-galacturonic acid dehydrogenase gene

Boer, H., Maaheimo, H., Koivula, A., Penttilä, M. & Richard, P., 1 Apr 2010, In : Applied Microbiology and Biotechnology. 86, 3, p. 901-909

Research output: Contribution to journalArticleScientificpeer-review

Agrobacterium tumefaciens
Oxidoreductases
NAD
uronate dehydrogenase
Lactones
61 Citations (Scopus)

Kinetic and biochemical properties of high and low redox potential laccases from fungal and plant origin

Frasconi, M., Favero, G., Boer, H., Koivula, A. & Mazzei, F., 2010, In : Biochimica et Biophysica Acta: Proteins and Proteomics. 1804, 4, p. 899-908 10 p.

Research output: Contribution to journalArticleScientificpeer-review

Laccase
Trametes
Oxidation-Reduction
Kinetics
Rhus
4 Citations (Scopus)

Performance of a printable enzymatic fuel cell: Study on mediated ThL laccase cathode

Tuurala, S., Smolander, M., Uotila, J., Kaukoniemi, O-V., Boer, H., Valkiainen, M., Vaari, A., Koivula, A. & Jenkins, P., 2010, In : ECS Transactions. 25, 33, p. 1-10 10 p.

Research output: Contribution to journalArticleScientificpeer-review

Enzymatic fuel cells
Cathodes
Application programs
Substrates
Enzymes