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2018
1 Citation (Scopus)
Xylans
Peeling
Fibers
Chemical analysis
Hydrolysis
2017
32 Citations (Scopus)

Development of a low-cost cellulase production process using Trichoderma reesei for Brazilian biorefineries

Ellilä, S., Fonseca, L., Uchima, C., Cota, J., Goldman, G. H., Saloheimo, M., Sacon, V. & Siika-Aho, M., 2017, In : Biotechnology for Biofuels. 10, 1, p. 30

Research output: Contribution to journalArticleScientificpeer-review

Open Access
Trichoderma
Cellulase
production cost
Saccharum
Costs and Cost Analysis
15 Citations (Scopus)

Enzymatic degradation of sulfite-pulped softwoods and the role of LPMOs

Chylenski, P., Petrovic, D. M., Müller, G., Dahlström, M., Bengtsson, O., Lersch, M., Siika-aho, M., Horn, S. J. & Eijsink, V. G. H., 11 Jul 2017, In : Biotechnology for Biofuels. 10, 1, 177.

Research output: Contribution to journalArticleScientificpeer-review

Open Access
Sulfites
Softwoods
sulfite
Polysaccharides
Mixed Function Oxygenases
10 Citations (Scopus)
Open Access
Enzymatic hydrolysis
Carbonization
Salix
hydrolysis
Biomass
11 Citations (Scopus)

Production of sugars from grass silage after steam explosion or soaking in aqueous ammonia

Niemi, P., Pihlajaniemi, V., Rinne, M. & Siika-aho, M., 1 Apr 2017, In : Industrial Crops and Products. 98, January, p. 93-99 7 p.

Research output: Contribution to journalArticleScientificpeer-review

explosions
grass silage
soaking
steam
ammonia
10 Citations (Scopus)

Purification and concentration of lignin from the spent liquor of the alkaline oxidation of woody biomass through membrane separation technology

Servaes, K., Varhimo, A., Dubreuil, M., Bulut, M., Vandezande, P., Siika-aho, M., Sirviö, J., Kruus, K., Porto-Carrero, W. & Bongers, M., 1 Nov 2017, In : Industrial Crops and Products. 106, p. 86-96 11 p.

Research output: Contribution to journalArticleScientificpeer-review

lignin
oxidation
biomass
softwood
pretreatment
2016

Comparison of four pre-treatment methods

Siika-aho, M., Leschinsky, M., van Groenestijn, J., Blomberg, D., Aasen, I. M., Dubreuil, M., Varhimo, A., Verges, M., Beckers, E., Johansson, E., Hytönen, E., Sirviö, J. & Kristiina, K., 2016.

Research output: Contribution to conferenceOther conference contributionProfessional

7 Citations (Scopus)

Effect of Microwave-Assisted Pretreatment Conditions on Hemicellulose Conversion and Enzymatic Hydrolysis of Norway Spruce

Kuittinen, S., Rodriguez, Y. P., Yang, M., Keinänen, M., Pastinen, O., Siika-aho, M., Turunen, O. & Pappinen, A., 1 Mar 2016, In : Bioenergy Research. 9, 1, p. 344-354 11 p.

Research output: Contribution to journalArticleScientificpeer-review

Enzymatic hydrolysis
enzymatic hydrolysis
hemicellulose
Picea abies
monosaccharides
7 Citations (Scopus)

Expression of Two Novel β-Glucosidases from Chaetomium atrobrunneum in Trichoderma reesei and Characterization of the Heterologous Protein Products

Colabardini, A. C., Valkonen, M., Huuskonen, A., Siika-aho, M., Koivula, A., Goldman, G. H. & Saloheimo, M., 1 Dec 2016, In : Molecular Biotechnology. 58, 12, p. 821-831 11 p.

Research output: Contribution to journalArticleScientificpeer-review

Chaetomium
Glucosidases
Trichoderma
Enzymes
Proteins
7 Citations (Scopus)

Utilization of recycled wood in biorefineries: preliminary results of steam explosion and ethanol/water organosolv pulping without a catalyst

Lesar, B., Humar, M., Hora, G., Hachmeister, P., Schmiedl, D., Pindel, E., Siika-aho, M. & Liitiä, T., 2016, In : European Journal of Wood and Wood Products. 74, 5, p. 711-723

Research output: Contribution to journalArticleScientificpeer-review

organosolv pulping
biorefining
explosions
Steam
catalysts
2015
21 Citations (Scopus)

Charge-Based Engineering of Hydrophobin HFBI: Effect on Interfacial Assembly and Interactions

Lienemann, M., Grunér, M. S., Paananen, A., Siika-Aho, M. & Linder, M. B., 27 Feb 2015, In : Biomacromolecules. 16, 4, p. 1283-1292 10 p.

Research output: Contribution to journalArticleScientificpeer-review

Proteins
Fungi
Adhesion
Viscoelasticity
Macromolecules

Sugars from biomass: High cellulose hydrolysability of oxygen alkali treated spruce, beech and wheat straw

Siika-aho, M., Varhimo, A., Sirviö, J. & Kristiina, K., 2015.

Research output: Contribution to conferenceConference articleProfessional

1 Citation (Scopus)

The essential role of plant cell wall degrading enzymes in the success of biorefineries: current status and future challenges

Silveira, M. H. L., Siika-Aho, M., Kruus, K., Garriga, L. M. & Ramos, L. P., 2015, Biofuels in Brazil: fundamental aspects, recent developments, and future perspectives. da Silva, S. S. & Chandel, A. K. (eds.). Springer, p. 151-172

Research output: Chapter in Book/Report/Conference proceedingChapter or book articleProfessional

biorefining
cell walls
fuel production
bioethanol
enzymatic hydrolysis
2014
18 Citations (Scopus)

Assessment of the enzymatic hydrolysis profile of cellulosic substrates based on reducing sugar release

Silveira, M. H. L., Aguiar, R. S., Siika-aho, M. & Ramos, L. P., 2014, In : Bioresource Technology. 151, p. 392-396

Research output: Contribution to journalArticleScientificpeer-review

Enzymatic hydrolysis
Sugars
Cellulase
hydrolysis
sugar
3 Citations (Scopus)

Hydrolysis and composition of recovered fibres fractionated from solid recovered fuel

Kemppainen, K., Siika-aho, M., Östman, A., Sipilä, E., Puranen, T., von Weymarn, N. & Kruus, K., 2014, In : Bioresource Technology. 169, p. 88-95 8 p.

Research output: Contribution to journalArticleScientificpeer-review

hydrolysis
Hydrolysis
Fibers
Chemical analysis
Carbohydrates
14 Citations (Scopus)

Mixtures of Thermostable Enzymes Show High Performance in Biomass Saccharification

Kallioinen, A., Puranen, T. & Siika-aho, M., 2014, In : Applied Biochemistry and Biotechnology. 173, 5, p. 1038-1056 19 p.

Research output: Contribution to journalArticleScientificpeer-review

Saccharification
Biomass
Enzymes
Bagasse
Hydrolysis
45 Citations (Scopus)

Spruce bark as an industrial source of condensed tannins and non-cellulosic sugars

Kemppainen, K., Siika-aho, M., Pattathi, S., Giovando, S. & Kruus, K., 2014, In : Industrial Crops and Products. 52, p. 158-168 11 p.

Research output: Contribution to journalArticleScientificpeer-review

proanthocyanidins
Picea
bark
sugars
tannins

Sugars from woody biomass: High cellulose hydrolysability of oxygen alkali treated spruce wood

Siika-aho, M., Määttänen, M., Liukkonen, S., Sirviö, J. & Varhimo, A., 2014.

Research output: Contribution to conferenceOther conference contributionProfessional

15 Citations (Scopus)

The role of endoglucanase and endoxylanase in liquefaction of hydrothermally pretreated wheat straw

Skovgaard, P. A., Thygesen, L. G., Jørgensen, H., Cardona, M., Tozzi, E., Mccarthy, M. & Siika-aho, M., 2014, In : Biotechnology Progress. 30, 4, p. 923-931 8 p.

Research output: Contribution to journalArticleScientificpeer-review

Endo-1,4-beta Xylanases
Cellulase
Viscosity
Triticum
Cellulases
2013
31 Citations (Scopus)

A novel alkaline oxidation pretreatment for spruce, birch and sugar cane bagasse

Kallioinen, A., Hakola, M., Riekkola, T., Repo, T., Leskelä, M., Von Weymarn, N. & Siika-aho, M., 2013, In : Bioresource Technology. 140, p. 414-420 6 p.

Research output: Contribution to journalArticleScientificpeer-review

Sugar cane
Bagasse
sugar cane
hydrolysis
Hydrolysis
74 Citations (Scopus)

Carbohydrate-binding modules (CBMs) revisited: reduced amount of water counterbalances the need for CBMs

Várnai, A., Siika-aho, M. & Viikari, L., 26 Feb 2013, In : Biotechnology for Biofuels. 6, p. 30 11 p.

Research output: Contribution to journalArticleScientificpeer-review

Open Access
Cellulases
Carbohydrates
carbohydrate
Water
substrate
11 Citations (Scopus)

Small-angle scattering study of structural changes in the microfibril network of nanocellulose during enzymatic hydrolysis

Penttilä, P. A., Várnai, A., Fernández, M., Kontro, I., Liljeström, V., Lindner, P., Siika-aho, M., Viikari, L. & Serimaa, R., 2013, In : Cellulose. 20, 3, p. 1031-1040 10 p.

Research output: Contribution to journalArticleScientificpeer-review

Enzymatic hydrolysis
Cellulose
Xylans
Scattering
Hydrolysis

Spruce bark biorefinery

Kemppainen, K., Niemelä, T., Inkinen, J., Uusitalo, J., Kaijaluoto, S., Sorsamäki, L., Aaltonen, O., Suurnäkki, A., Peltonen, S., Mikkonen, H., Grönberg, V., Kruus, K., Nakari-Setälä, T. & Siika-aho, M., 2013, Proceedings of the 21st European Biomass Conference. p. 540-543

Research output: Chapter in Book/Report/Conference proceedingConference article in proceedingsScientific

biorefining
Picea
bark
tannins
forest industries
40 Citations (Scopus)

Xylanase XYN IV from Trichoderma reesei showing exo- and endo-xylanase activity

Tenkanen, M., Vršanská, M., Siika-Aho, M., Wong, D. W., Puchart, V., Penttilä, M., Saloheimo, M. & Biely, P., 1 Jan 2013, In : FEBS Journal. 280, 1, p. 285-301

Research output: Contribution to journalArticleScientificpeer-review

Trichoderma
Xylose
Enzymes
Xylans
glucuronoxylan xylanohydrolase
52 Citations (Scopus)

Xylan as limiting factor in enzymatic hydrolysis of nanocellulose

Penttilä, P. A., Varnai, A., Pere, J., Tammelin, T., Salmén, L., Siika-aho, M., Viikari, L. & Serimaa, R., 2013, In : Bioresource Technology. 129, p. 135-141 7 p.

Research output: Contribution to journalArticleScientificpeer-review

Xylans
Enzymatic hydrolysis
limiting factor
hydrolysis
cellulose
2012

Alkaline oxidation pretreatment of wood materials to produce ethanol

Kallioinen, A., Siika-aho, M., Hytönen, E., Hakola, M., Rovio, S., Tamminen, T., Repo, T., Leskelä, M. & von Weymarn, N., 2012, NWBC 2012: The 4th Nordic Wood Biorefinery Conference. Niemelä, K. (ed.). Espoo: VTT Technical Research Centre of Finland, p. 327-328 (VTT Technology; No. 53).

Research output: Chapter in Book/Report/Conference proceedingConference article in proceedingsProfessional

Open Access
Fractionation
Wood
Ethanol
Oxidation
Enzymatic hydrolysis
15 Citations (Scopus)

Catalysed alkaline oxidation as a wood fractionation technique

Rovio, S., Kallioinen, A., Tamminen, T., Hakola, M., Leskelä, M. & Siika-aho, M., 2012, In : BioResources. 7, 1, p. 756-776 20 p.

Research output: Contribution to journalArticleScientificpeer-review

Open Access
Fractionation
Wood
fractionation
oxidation
Oxidation

Continuous pilot scale process demonstrating waste fibre as a feasible feedstock to ethanol and biogas production

Kemppainen, K., Ranta, L., Sipilä, E., Östman, A., Vehmaanperä, J., Puranen, T., Langfelder, K., Hannula, J., Kallioinen, A., Siika-aho, M., Sipilä, K. & von Weymarn, N., 2012.

Research output: Contribution to conferenceConference articleScientific

Biogas
Feedstocks
Ethanol
Fibers
Hydrolysis

Enzymes - tools to upgrade biomass to high-value products

Grönqvist, S., Pere, J., Siika-aho, M., Suurnäkki, A. & Hakala, T. K., 2012, NWBC 2012: The 4th Nordic Wood Biorefinery Conference. Niemelä, K. (ed.). Espoo: VTT Technical Research Centre of Finland, p. 329-330 (VTT Technology; No. 53).

Research output: Chapter in Book/Report/Conference proceedingConference article in proceedingsProfessional

Open Access
enzyme
biomass
natural resource
fossil
climate change
19 Citations (Scopus)

Ethanol and biogas production from waste fibre and fibre sludge: The FibreEtOH concept

Kemppainen, K., Ranta, L., Sipilä, E., Östman, A., Vehmaanperä, J., Puranen, T., Langfelder, K., Hannula, J., Kallioinen, A., Siika-aho, M., Sipilä, K. & von Weymarn, N., 2012, In : Biomass and Bioenergy. 46, p. 60-69 10 p.

Research output: Contribution to journalArticleScientificpeer-review

Biogas
biogas
sludge
Fermentation
fermentation
42 Citations (Scopus)

Hot water extraction and steam explosion as pretreatments for ethanol production from spruce bark

Kemppainen, K., Inkinen, J., Uusitalo, J., Nakari-Setälä, T. & Siika-aho, M., 2012, In : Bioresource Technology. 117, p. 131-139

Research output: Contribution to journalArticleScientificpeer-review

Steam
bark
Explosions
explosion
ethanol
23 Citations (Scopus)

Reed canary grass as a feedstock for 2nd generation bioethanol production

Kallioinen, A., Uusitalo, J., Pahkala, K., Kontturi, M., Viikari, L., von Weymarn, N. & Siika-aho, M., 2012, In : Bioresource Technology. 123, p. 669-672 3 p.

Research output: Contribution to journalArticleScientificpeer-review

Bioethanol
Feedstocks
hydrolysis
Hydrolysis
grass
4 Citations (Scopus)

Susceptibility of pretreated wood sections of Norway spruce (Picea abies) clones to enzymatic hydrolysis

Rodriguez, Y. P., Puhakka-Tarvainen, H., Pastinen, O., Siika-aho, M., Alvila, L., Turunen, O., Morales, L. & Pappinen, A., 2012, In : Canadian Journal of Forest Research. 42, 1, p. 38-46 9 p.

Research output: Contribution to journalArticleScientificpeer-review

enzymatic hydrolysis
Picea abies
clone
hydrolysis
reducing sugars
2011
53 Citations (Scopus)

Comparison of the synergistic action of two thermostable xylanases from GH families 10 and 11 with thermostable cellulases in lignocellulose hydrolysis

Zhang, J., Tuomainen, P., Siika-aho, M. & Viikari, L., 2011, In : Bioresource Technology. 102, 19, p. 9090-9095 6 p.

Research output: Contribution to journalArticleScientificpeer-review

Cellulases
Xylans
Straw
hydrolysis
Hydrolysis

Enzymatic hydrolysis and ethanol production of hot water extracted spruce bark

Nyman, K., Uusitalo, J., Inkinen, J., Siika-aho, M. & Nakari-Setälä, T., 2011.

Research output: Contribution to conferenceConference PosterScientific

97 Citations (Scopus)

High level secretion of cellobiohydrolases by Saccharomyces cerevisiae

Ilmén, M., Den Haan, R., Brevnova, E., McBride, J., Wiswall, E., Froehlich, A., Koivula, A., Voutilainen, S. P., Siika-Aho, M., La Grange, D. C., Thorngren, N., Ahlgren, S., Mellon, M., Deleault, K., Rajgarhia, V., Van Zyl, W. H. & Penttilä, M., 14 Sep 2011, In : Biotechnology for Biofuels. 4, 30.

Research output: Contribution to journalArticleScientificpeer-review

Open Access
Cellulose 1,4-beta-Cellobiosidase
secretion
Yeast
Saccharomyces cerevisiae
cellulose

Isolation of oligosaccharides and xylan from kraft pulp by enzyme-aided alkaline extraction

Hakala, T., Mikkelson, A., Manelius, R., Willför, S. & Siika-aho, M., 2011, 2nd International Polysaccharide Conference EPNOE 2011: Book of abstracts. Wageningen Academic Publishers, 16

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsScientific

26 Citations (Scopus)

Proteomic and functional analysis of the cellulase system expressed by Postia placenta during brown rot of solid wood

Ryu, J. S., Shary, S., Houtman, C. J., Panisko, E. A., Korripally, P., St. John, F. J., Crooks, C., Siika-aho, M., Magnuson, J. K. & Hammel, K. E., 2011, In : Applied and Environmental Microbiology. 77, 22, p. 7933-7941 9 p.

Research output: Contribution to journalArticleScientificpeer-review

Rhodonia placenta
cellulases
proteomics
Cellulase
Systems Analysis
82 Citations (Scopus)

Synergistic action of xylanase and mannanase improves the total hydrolysis of softwood

Várnai, A., Huikko, L., Pere, J., Siika-aho, M. & Viikari, L., 2011, In : Bioresource Technology. 102, 19, p. 9096-9104 9 p.

Research output: Contribution to journalArticleScientificpeer-review

Softwoods
hydrolysis
Hydrolysis
Xylans
Enzymes
41 Citations (Scopus)

Thermostable endoglucanases in the liquefaction of hydrothermally pretreated wheat straw

Szijártó, N., Horan, E., Zhang, J., Puranen, T., Siika-aho, M. & Viikari, L., 2011, In : Biotechnology for Biofuels. 4, 2

Research output: Contribution to journalArticleScientificpeer-review

Open Access
Cellulase
Straw
Liquefaction
liquefaction
straw
86 Citations (Scopus)

The role of acetyl xylan esterase in the solubilization of xylan and enzymatic hydrolysis of wheat straw and giant reed

Zhang, J., Siika-aho, M., Tenkanen, M. & Viikari, L., 2011, In : Biotechnology for Biofuels. 4, 60

Research output: Contribution to journalArticleScientificpeer-review

Open Access
acetylxylan esterase
Xylans
Enzymatic hydrolysis
Straw
solubilization
2010
66 Citations (Scopus)

Adsorption of monocomponent enzymes in enzyme mixture analyzed quantitatively during hydrolysis of lignocellulose substrates

Varnai, A., Viikari, L., Marjamaa, K. & Siika-aho, M., 2010, In : Bioresource Technology. 102, 2, p. 1220-1227 8 p.

Research output: Contribution to journalArticleScientificpeer-review

hydrolysis
Hydrolysis
Enzymes
enzyme
adsorption
42 Citations (Scopus)

Changes in submicrometer structure of enzymatically hydrolyzed microcrystalline cellulose

Penttilä, P. A., Varnai, A., Leppänen, K., Peura, M., Kallonen, A., Jääskeläinen, P., Lucenius, J., Ruokolainen, J., Siika-aho, M., Viikari, L. & Serimaa, R., 2010, In : Biomacromolecules. 11, 4, p. 1111-1117 7 p.

Research output: Contribution to journalArticleScientificpeer-review

X ray scattering
Cellulose
Enzymes
Transmission electron microscopy
Enzymatic hydrolysis
62 Citations (Scopus)

Characterisation of specific activities and hydrolytic properties of cell-wall-degrading enzymes produced by Trichoderma reesei Rut C30 on different carbon sources

Sipos, B., Bénko, Z., Dienes, D., Réczey, K., Viikari, L. & Siika-aho, M., 2010, In : Applied Biochemistry and Biotechnology. 161, 1-8, p. 347-364 18 p.

Research output: Contribution to journalArticleScientificpeer-review

Glucosidases
Trichoderma
Xylans
Cell Wall
Carbon

Characterization of two thermostable xylanases as accessory enzymes in lignocelluloses hydrolysis

Zhang, J., Szijártó, N., Maijala, P., Siika-aho, M., Puranen, T., Tenkanen, M. & Viikari, L., 2010, Lignobiotech One Symposium: 1st Symposium on Biotechnology Applied to Lignocelluloses : March 28th - April 1st 2010, Reims, France. Presses universitaires de Reims

Research output: Chapter in Book/Report/Conference proceedingConference article in proceedingsScientific

15 Citations (Scopus)

Effect of acid and enzymatic treatments of TCF dissolving pulp on the properties of wet spun cellulosic fibres

Vehviläinen, M., Kamppuri, T., Nousiainen, P., Kallioinen, A., Siika-aho, M., Christoffersson, K. E., Rom, M. & Janicki, J., 2010, In : Cellulose chemistry and technology. 44, 4-6, p. 147-151 5 p.

Research output: Contribution to journalArticleScientificpeer-review

Open Access
Cellulose
Pulp
Acids
Fibers
Molecular weight

Enzymatic activation of softwood dissolving pulps

Tamminen, T., Siika-aho, M., Liitiä, T., Adden, R., Parviainen, H. & Maunu, S-L., 2010, In : Journal of Biotechnology. 150, Supplement, p. 508 [P-P&F.97].

Research output: Contribution to journalOther journal contributionScientificpeer-review

45 Citations (Scopus)

Hydrolysis efficiency and enzyme adsorption on steam pretreated spruce in the presence of poly(ethylene glycol)

Sipos, B., Dienes, D., Schleicher, Á., Perazzini, R., Crestini, C., Siika-aho, M. & Réczey, K., 2010, In : Enzyme and Microbial Technology. 47, 3, p. 84-90 7 p.

Research output: Contribution to journalArticleScientificpeer-review

Ethylene Glycol
Steam
Polyethylene glycols
Adsorption
Hydrolysis
1 Citation (Scopus)

Industrial Biotechnology in the Paper and Pulp Sector

Viikari, L., Grönqvist, S., Kruus, K., Pere, J., Siika-aho, M. & Suurnäkki, A., 2010, Industrial Biotechnology: Sustainable Growth and Economic Success. Soetaert, W. & Vandamme, E. J. (eds.). Weinheim, p. 385-412

Research output: Chapter in Book/Report/Conference proceedingChapter or book articleScientificpeer-review

19 Citations (Scopus)

Liberation of cellulose from the lignin cage: A catalytic pretreatment method for the production of cellulosic ethanol

Hakola, M., Kallioinen, A., Kemell, M., Lahtinen, P., Lankinen, E., Leskelä, M., Repo, T., Riekkola, T., Siika-aho, M., Uusitalo, J., Vuorela, S. & von Weymarn, N., 2010, In : ChemSusChem. 3, 10, p. 1142-1145 4 p.

Research output: Contribution to journalArticleScientificpeer-review

Cellulosic ethanol
Lignin
Cellulose
lignin
cellulose