Abstract
The immobilization of bioactive molecules onto nanocellulose leads to constructs that combine the properties of the grafted compounds with the biocompatibility and low cytotoxicity of cellulose carriers and the advantages given by their nanometer dimensions. However, the methods commonly used for protein grafting suffer from lack of selectivity, long reaction times, nonphysiological pH ranges and solvents, and the necessity to develop a tailor-made reaction strategy for each individual case. To overcome these restrictions, a generic two-step procedure was developed that takes advantage of the highly efficient oxime ligation combined with enzyme-mediated protein coupling onto the surface of peptide-modified crystalline nanocellulose. The described method is based on efficient and orthogonal transformations, requires no organic solvents, and takes place under physiological conditions. Being site-directed and regiospecific, it could be applied to a vast number of functional proteins.
| Original language | English |
|---|---|
| Pages (from-to) | 12618-12623 |
| Number of pages | 6 |
| Journal | Angewandte Chemie - International Edition |
| Volume | 53 |
| Issue number | 46 |
| DOIs | |
| Publication status | Published - 10 Nov 2014 |
| MoE publication type | A1 Journal article-refereed |
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Keywords
- Bioorthogonal protein immobilization
- Cellulose nanocrystals
- Enzyme catalysis
- Immobilization
- Ligation
Cite this
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A chemoenzymatic approach to protein immobilization onto crystalline cellulose nanoscaffolds. / Uth, Christina; Zielonka, Stefan; Hörner, Sebastian; Rasche, Nicolas; Plog, Andreas; Orelma, Hannes; Avrutina, Olga; Zhang, Kai; Kolmar, Harald.
In: Angewandte Chemie - International Edition, Vol. 53, No. 46, 10.11.2014, p. 12618-12623.Research output: Contribution to journal › Article › Scientific › peer-review
TY - JOUR
T1 - A chemoenzymatic approach to protein immobilization onto crystalline cellulose nanoscaffolds
AU - Uth, Christina
AU - Zielonka, Stefan
AU - Hörner, Sebastian
AU - Rasche, Nicolas
AU - Plog, Andreas
AU - Orelma, Hannes
AU - Avrutina, Olga
AU - Zhang, Kai
AU - Kolmar, Harald
PY - 2014/11/10
Y1 - 2014/11/10
N2 - The immobilization of bioactive molecules onto nanocellulose leads to constructs that combine the properties of the grafted compounds with the biocompatibility and low cytotoxicity of cellulose carriers and the advantages given by their nanometer dimensions. However, the methods commonly used for protein grafting suffer from lack of selectivity, long reaction times, nonphysiological pH ranges and solvents, and the necessity to develop a tailor-made reaction strategy for each individual case. To overcome these restrictions, a generic two-step procedure was developed that takes advantage of the highly efficient oxime ligation combined with enzyme-mediated protein coupling onto the surface of peptide-modified crystalline nanocellulose. The described method is based on efficient and orthogonal transformations, requires no organic solvents, and takes place under physiological conditions. Being site-directed and regiospecific, it could be applied to a vast number of functional proteins.
AB - The immobilization of bioactive molecules onto nanocellulose leads to constructs that combine the properties of the grafted compounds with the biocompatibility and low cytotoxicity of cellulose carriers and the advantages given by their nanometer dimensions. However, the methods commonly used for protein grafting suffer from lack of selectivity, long reaction times, nonphysiological pH ranges and solvents, and the necessity to develop a tailor-made reaction strategy for each individual case. To overcome these restrictions, a generic two-step procedure was developed that takes advantage of the highly efficient oxime ligation combined with enzyme-mediated protein coupling onto the surface of peptide-modified crystalline nanocellulose. The described method is based on efficient and orthogonal transformations, requires no organic solvents, and takes place under physiological conditions. Being site-directed and regiospecific, it could be applied to a vast number of functional proteins.
KW - Bioorthogonal protein immobilization
KW - Cellulose nanocrystals
KW - Enzyme catalysis
KW - Immobilization
KW - Ligation
UR - http://www.scopus.com/inward/record.url?scp=84919328850&partnerID=8YFLogxK
U2 - 10.1002/anie.201404616
DO - 10.1002/anie.201404616
M3 - Article
VL - 53
SP - 12618
EP - 12623
JO - Angewandte Chemie: International Edition
JF - Angewandte Chemie: International Edition
SN - 1433-7851
IS - 46
ER -