A chemoenzymatic approach to protein immobilization onto crystalline cellulose nanoscaffolds

Christina Uth, Stefan Zielonka, Sebastian Hörner, Nicolas Rasche, Andreas Plog, Hannes Orelma, Olga Avrutina, Kai Zhang, Harald Kolmar

Research output: Contribution to journalArticleScientificpeer-review

42 Citations (Scopus)

Abstract

The immobilization of bioactive molecules onto nanocellulose leads to constructs that combine the properties of the grafted compounds with the biocompatibility and low cytotoxicity of cellulose carriers and the advantages given by their nanometer dimensions. However, the methods commonly used for protein grafting suffer from lack of selectivity, long reaction times, nonphysiological pH ranges and solvents, and the necessity to develop a tailor-made reaction strategy for each individual case. To overcome these restrictions, a generic two-step procedure was developed that takes advantage of the highly efficient oxime ligation combined with enzyme-mediated protein coupling onto the surface of peptide-modified crystalline nanocellulose. The described method is based on efficient and orthogonal transformations, requires no organic solvents, and takes place under physiological conditions. Being site-directed and regiospecific, it could be applied to a vast number of functional proteins.

Original languageEnglish
Pages (from-to)12618-12623
Number of pages6
JournalAngewandte Chemie: International Edition
Volume53
Issue number46
DOIs
Publication statusPublished - 10 Nov 2014
MoE publication typeA1 Journal article-refereed

Keywords

  • Bioorthogonal protein immobilization
  • Cellulose nanocrystals
  • Enzyme catalysis
  • Immobilization
  • Ligation

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    Uth, C., Zielonka, S., Hörner, S., Rasche, N., Plog, A., Orelma, H., Avrutina, O., Zhang, K., & Kolmar, H. (2014). A chemoenzymatic approach to protein immobilization onto crystalline cellulose nanoscaffolds. Angewandte Chemie: International Edition, 53(46), 12618-12623. https://doi.org/10.1002/anie.201404616