A disposable, roll-to-roll hot-embossed inertial microfluidic device for size-based sorting of microbeads and cells

Xiao Wang, Christina Liedert, Ralph Liedert, Ian Paputsky (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

24 Citations (Scopus)

Abstract

Inertial microfluidics has been a highly active area of research in recent years for high-throughput focusing and sorting of synthetic and biological microparticles. However, existing inertial microfluidic devices always rely on microchannels with high-aspect-ratio geometries (channel width w <channel height h) and small cross-sections (w * h <50 * 100 µm2). Such deep and small structures increase fabrication difficulty and can limit manufacturing by large-scale and high-throughput production approaches such as roll-to-roll (R2R) hot embossing. In this work, we present a novel inertial microfluidic device using only a simple and low-aspect-ratio (LAR) straight microchannel (w > h) to achieve size-based sorting of microparticles and cells. The simple LAR geometry of the device enables successful high-throughput fabrication using R2R hot embossing. With optimized flow conditions and channel dimensions, we demonstrate continuous sorting of a mixture of 15 µm and 10 µm diameter microbeads with >97% sorting efficiency using the low-cost and disposable R2R chip. We further demonstrate size-based sorting of bovine white blood cells, demonstrating the ability to process real cellular samples in our R2R chip. We envision that this R2R hot-embossed inertial microfluidic chip will serve as a powerful yet low-cost and disposable tool for size-based sorting of synthetic microparticles in industrial applications or cellular samples in cell biology research and clinical diagnostics.
Original languageEnglish
Pages (from-to)1821-1830
JournalLab on a Chip
Volume16
Issue number10
DOIs
Publication statusPublished - 2016
MoE publication typeA1 Journal article-refereed

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Lab-On-A-Chip Devices
Microfluidics
Microspheres
Sorting
Costs and Cost Analysis
Research
Cell Biology
Aspect ratio
Leukocytes
Throughput
Microchannels
Equipment and Supplies
Cytology
Fabrication
Geometry
Industrial applications
Costs
Blood
Cells

Cite this

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title = "A disposable, roll-to-roll hot-embossed inertial microfluidic device for size-based sorting of microbeads and cells",
abstract = "Inertial microfluidics has been a highly active area of research in recent years for high-throughput focusing and sorting of synthetic and biological microparticles. However, existing inertial microfluidic devices always rely on microchannels with high-aspect-ratio geometries (channel width w <channel height h) and small cross-sections (w * h <50 * 100 µm2). Such deep and small structures increase fabrication difficulty and can limit manufacturing by large-scale and high-throughput production approaches such as roll-to-roll (R2R) hot embossing. In this work, we present a novel inertial microfluidic device using only a simple and low-aspect-ratio (LAR) straight microchannel (w > h) to achieve size-based sorting of microparticles and cells. The simple LAR geometry of the device enables successful high-throughput fabrication using R2R hot embossing. With optimized flow conditions and channel dimensions, we demonstrate continuous sorting of a mixture of 15 µm and 10 µm diameter microbeads with >97{\%} sorting efficiency using the low-cost and disposable R2R chip. We further demonstrate size-based sorting of bovine white blood cells, demonstrating the ability to process real cellular samples in our R2R chip. We envision that this R2R hot-embossed inertial microfluidic chip will serve as a powerful yet low-cost and disposable tool for size-based sorting of synthetic microparticles in industrial applications or cellular samples in cell biology research and clinical diagnostics.",
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A disposable, roll-to-roll hot-embossed inertial microfluidic device for size-based sorting of microbeads and cells. / Wang, Xiao; Liedert, Christina; Liedert, Ralph; Paputsky, Ian (Corresponding Author).

In: Lab on a Chip, Vol. 16, No. 10, 2016, p. 1821-1830.

Research output: Contribution to journalArticleScientificpeer-review

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AB - Inertial microfluidics has been a highly active area of research in recent years for high-throughput focusing and sorting of synthetic and biological microparticles. However, existing inertial microfluidic devices always rely on microchannels with high-aspect-ratio geometries (channel width w <channel height h) and small cross-sections (w * h <50 * 100 µm2). Such deep and small structures increase fabrication difficulty and can limit manufacturing by large-scale and high-throughput production approaches such as roll-to-roll (R2R) hot embossing. In this work, we present a novel inertial microfluidic device using only a simple and low-aspect-ratio (LAR) straight microchannel (w > h) to achieve size-based sorting of microparticles and cells. The simple LAR geometry of the device enables successful high-throughput fabrication using R2R hot embossing. With optimized flow conditions and channel dimensions, we demonstrate continuous sorting of a mixture of 15 µm and 10 µm diameter microbeads with >97% sorting efficiency using the low-cost and disposable R2R chip. We further demonstrate size-based sorting of bovine white blood cells, demonstrating the ability to process real cellular samples in our R2R chip. We envision that this R2R hot-embossed inertial microfluidic chip will serve as a powerful yet low-cost and disposable tool for size-based sorting of synthetic microparticles in industrial applications or cellular samples in cell biology research and clinical diagnostics.

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