A fingerprint database for characterization and identification of microbial contaminants

Maija-Liisa Suihko, Erna Storgårds, Auli Haikara

Research output: Chapter in Book/Report/Conference proceedingConference article in proceedingsScientificpeer-review

Abstract

Ribotyping of brewery contaminants was carried out by automated ribotyping with the restriction enzymes EcoRI, PvuII and PstI. A database was created mainly including fingerprints of Lactobacillus spp., Pediococcus spp., Pectinatus spp., Megasphaera cerevisiae and Obesumbacterium proteus, but also of other lactic acid bacteria, enterobacteria, acetic acid bacteria, clostridia and pseudomonads. The most important property of ribotyping to characterize strains below species level, has been successfully applied in tracing of contamination routes and in detection of troublesome "house types" in industrial processes. The database created is extremely useful for reliable and rapid identification of bacteria isolated from brewing and malting industry.
Original languageEnglish
Title of host publicationProceedings of the 29th EBC Congress: Dublin 2003
Subtitle of host publicationEuropean Brewery Convention, 17-22 May 2003, Dublin, Ireland
Place of PublicationNurnberg
PublisherFachverlag Hans Carl
Pages1121 - 1127
Editioncd-rom
ISBN (Print)90-70143-22-4
Publication statusPublished - 2003
MoE publication typeA4 Article in a conference publication

Fingerprint

microbial contamination
DNA fingerprinting
Obesumbacterium proteus
Megasphaera cerevisiae
Pectinatus
acetic acid bacteria
Pediococcus
brewing industry
malting
brewing
Lactobacillus
lactic acid bacteria
industry
bacteria
enzymes

Keywords

  • beer spoilage organism
  • database
  • identification of bacteria

Cite this

Suihko, M-L., Storgårds, E., & Haikara, A. (2003). A fingerprint database for characterization and identification of microbial contaminants. In Proceedings of the 29th EBC Congress: Dublin 2003: European Brewery Convention, 17-22 May 2003, Dublin, Ireland (cd-rom ed., pp. 1121 - 1127). Nurnberg: Fachverlag Hans Carl.
Suihko, Maija-Liisa ; Storgårds, Erna ; Haikara, Auli. / A fingerprint database for characterization and identification of microbial contaminants. Proceedings of the 29th EBC Congress: Dublin 2003: European Brewery Convention, 17-22 May 2003, Dublin, Ireland. cd-rom. ed. Nurnberg : Fachverlag Hans Carl, 2003. pp. 1121 - 1127
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Suihko, M-L, Storgårds, E & Haikara, A 2003, A fingerprint database for characterization and identification of microbial contaminants. in Proceedings of the 29th EBC Congress: Dublin 2003: European Brewery Convention, 17-22 May 2003, Dublin, Ireland. cd-rom edn, Fachverlag Hans Carl, Nurnberg, pp. 1121 - 1127.

A fingerprint database for characterization and identification of microbial contaminants. / Suihko, Maija-Liisa; Storgårds, Erna; Haikara, Auli.

Proceedings of the 29th EBC Congress: Dublin 2003: European Brewery Convention, 17-22 May 2003, Dublin, Ireland. cd-rom. ed. Nurnberg : Fachverlag Hans Carl, 2003. p. 1121 - 1127.

Research output: Chapter in Book/Report/Conference proceedingConference article in proceedingsScientificpeer-review

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AU - Suihko, Maija-Liisa

AU - Storgårds, Erna

AU - Haikara, Auli

PY - 2003

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N2 - Ribotyping of brewery contaminants was carried out by automated ribotyping with the restriction enzymes EcoRI, PvuII and PstI. A database was created mainly including fingerprints of Lactobacillus spp., Pediococcus spp., Pectinatus spp., Megasphaera cerevisiae and Obesumbacterium proteus, but also of other lactic acid bacteria, enterobacteria, acetic acid bacteria, clostridia and pseudomonads. The most important property of ribotyping to characterize strains below species level, has been successfully applied in tracing of contamination routes and in detection of troublesome "house types" in industrial processes. The database created is extremely useful for reliable and rapid identification of bacteria isolated from brewing and malting industry.

AB - Ribotyping of brewery contaminants was carried out by automated ribotyping with the restriction enzymes EcoRI, PvuII and PstI. A database was created mainly including fingerprints of Lactobacillus spp., Pediococcus spp., Pectinatus spp., Megasphaera cerevisiae and Obesumbacterium proteus, but also of other lactic acid bacteria, enterobacteria, acetic acid bacteria, clostridia and pseudomonads. The most important property of ribotyping to characterize strains below species level, has been successfully applied in tracing of contamination routes and in detection of troublesome "house types" in industrial processes. The database created is extremely useful for reliable and rapid identification of bacteria isolated from brewing and malting industry.

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KW - database

KW - identification of bacteria

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BT - Proceedings of the 29th EBC Congress: Dublin 2003

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Suihko M-L, Storgårds E, Haikara A. A fingerprint database for characterization and identification of microbial contaminants. In Proceedings of the 29th EBC Congress: Dublin 2003: European Brewery Convention, 17-22 May 2003, Dublin, Ireland. cd-rom ed. Nurnberg: Fachverlag Hans Carl. 2003. p. 1121 - 1127