A fluoroimmunoassay based on immunoliposomes containing genetically engineered lipid-tagged antibody

Eiry Kobatake, Hiroyuki Sasakura, Tetsuya Haruyama, Marja-Leena Laukkanen, Kari Keinänen, Masuo Aizawa (Corresponding Author)

    Research output: Contribution to journalArticleScientificpeer-review

    15 Citations (Scopus)

    Abstract

    Immunoliposomes were prepared by using biosynthetically lipid-tagged anti-2-phenyloxazolone single-chain antibody. Carboxyfluorescein as a fluorescent marker was encapsulated in the immunoliposomes. Some conditions for fluoroimmunoassay using the immunoliposomes were optimized by binding assays with hapten-coated microtiter wells. A competitive fluoroimmunoassay for the caproic acid conjugate of 2-phenyloxazolone as a model antigen was performed with the immunoliposomes. In the optimized assay conditions, antigen could be determined in the concentration range from 10-7 to 10-9 M.

    Original languageEnglish
    Pages (from-to)1295 - 1298
    Number of pages4
    JournalAnalytical Chemistry
    Volume69
    Issue number7
    DOIs
    Publication statusPublished - 1997
    MoE publication typeA1 Journal article-refereed

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