A fluoroimmunoassay based on immunoliposomes containing genetically engineered lipid-tagged antibody

Eiry Kobatake, Hiroyuki Sasakura, Tetsuya Haruyama, Marja-Leena Laukkanen, Kari Keinänen, Masuo Aizawa (Corresponding Author)

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Abstract

Immunoliposomes were prepared by using biosynthetically lipid-tagged anti-2-phenyloxazolone single-chain antibody. Carboxyfluorescein as a fluorescent marker was encapsulated in the immunoliposomes. Some conditions for fluoroimmunoassay using the immunoliposomes were optimized by binding assays with hapten-coated microtiter wells. A competitive fluoroimmunoassay for the caproic acid conjugate of 2-phenyloxazolone as a model antigen was performed with the immunoliposomes. In the optimized assay conditions, antigen could be determined in the concentration range from 10-7 to 10-9 M.

Original languageEnglish
Pages (from-to)1295 - 1298
Number of pages4
JournalAnalytical Chemistry
Volume69
Issue number7
DOIs
Publication statusPublished - 1997
MoE publication typeA1 Journal article-refereed

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Kobatake, E., Sasakura, H., Haruyama, T., Laukkanen, M-L., Keinänen, K., & Aizawa, M. (1997). A fluoroimmunoassay based on immunoliposomes containing genetically engineered lipid-tagged antibody. Analytical Chemistry, 69(7), 1295 - 1298. https://doi.org/10.1021/ac961162+