A fluoroimmunoassay based on immunoliposomes containing genetically engineered lipid-tagged antibody

Eiry Kobatake, Hiroyuki Sasakura, Tetsuya Haruyama, Marja-Leena Laukkanen, Kari Keinänen, Masuo Aizawa (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

14 Citations (Scopus)

Abstract

Immunoliposomes were prepared by using biosynthetically lipid-tagged anti-2-phenyloxazolone single-chain antibody. Carboxyfluorescein as a fluorescent marker was encapsulated in the immunoliposomes. Some conditions for fluoroimmunoassay using the immunoliposomes were optimized by binding assays with hapten-coated microtiter wells. A competitive fluoroimmunoassay for the caproic acid conjugate of 2-phenyloxazolone as a model antigen was performed with the immunoliposomes. In the optimized assay conditions, antigen could be determined in the concentration range from 10-7 to 10-9 M.

Original languageEnglish
Pages (from-to)1295 - 1298
Number of pages4
JournalAnalytical Chemistry
Volume69
Issue number7
DOIs
Publication statusPublished - 1997
MoE publication typeA1 Journal article-refereed

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Assays
Lipids
Antigens
Single-Chain Antibodies
Antibodies
Haptens
2-phenyloxazolone
6-carboxyfluorescein
hexanoic acid

Cite this

Kobatake, E., Sasakura, H., Haruyama, T., Laukkanen, M-L., Keinänen, K., & Aizawa, M. (1997). A fluoroimmunoassay based on immunoliposomes containing genetically engineered lipid-tagged antibody. Analytical Chemistry, 69(7), 1295 - 1298. https://doi.org/10.1021/ac961162+
Kobatake, Eiry ; Sasakura, Hiroyuki ; Haruyama, Tetsuya ; Laukkanen, Marja-Leena ; Keinänen, Kari ; Aizawa, Masuo. / A fluoroimmunoassay based on immunoliposomes containing genetically engineered lipid-tagged antibody. In: Analytical Chemistry. 1997 ; Vol. 69, No. 7. pp. 1295 - 1298.
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abstract = "Immunoliposomes were prepared by using biosynthetically lipid-tagged anti-2-phenyloxazolone single-chain antibody. Carboxyfluorescein as a fluorescent marker was encapsulated in the immunoliposomes. Some conditions for fluoroimmunoassay using the immunoliposomes were optimized by binding assays with hapten-coated microtiter wells. A competitive fluoroimmunoassay for the caproic acid conjugate of 2-phenyloxazolone as a model antigen was performed with the immunoliposomes. In the optimized assay conditions, antigen could be determined in the concentration range from 10-7 to 10-9 M.",
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Kobatake, E, Sasakura, H, Haruyama, T, Laukkanen, M-L, Keinänen, K & Aizawa, M 1997, 'A fluoroimmunoassay based on immunoliposomes containing genetically engineered lipid-tagged antibody', Analytical Chemistry, vol. 69, no. 7, pp. 1295 - 1298. https://doi.org/10.1021/ac961162+

A fluoroimmunoassay based on immunoliposomes containing genetically engineered lipid-tagged antibody. / Kobatake, Eiry; Sasakura, Hiroyuki; Haruyama, Tetsuya; Laukkanen, Marja-Leena; Keinänen, Kari; Aizawa, Masuo (Corresponding Author).

In: Analytical Chemistry, Vol. 69, No. 7, 1997, p. 1295 - 1298.

Research output: Contribution to journalArticleScientificpeer-review

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AU - Kobatake, Eiry

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AU - Keinänen, Kari

AU - Aizawa, Masuo

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AB - Immunoliposomes were prepared by using biosynthetically lipid-tagged anti-2-phenyloxazolone single-chain antibody. Carboxyfluorescein as a fluorescent marker was encapsulated in the immunoliposomes. Some conditions for fluoroimmunoassay using the immunoliposomes were optimized by binding assays with hapten-coated microtiter wells. A competitive fluoroimmunoassay for the caproic acid conjugate of 2-phenyloxazolone as a model antigen was performed with the immunoliposomes. In the optimized assay conditions, antigen could be determined in the concentration range from 10-7 to 10-9 M.

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Kobatake E, Sasakura H, Haruyama T, Laukkanen M-L, Keinänen K, Aizawa M. A fluoroimmunoassay based on immunoliposomes containing genetically engineered lipid-tagged antibody. Analytical Chemistry. 1997;69(7):1295 - 1298. https://doi.org/10.1021/ac961162+