A fluoroimmunoassay based on immunoliposomes containing genetically engineered lipid-tagged antibody

Eiry Kobatake; Hiroyuki Sasakura; Tetsuya Haruyama; Marja-Leena Laukkanen; Kari Keinänen; Masuo Aizawa

doi:10.1021/ac961162+

A fluoroimmunoassay based on immunoliposomes containing genetically engineered lipid-tagged antibody

Eiry Kobatake
, Hiroyuki Sasakura
, Tetsuya Haruyama
, Marja-Leena Laukkanen
, Kari Keinänen
, Masuo Aizawa^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Scientific › peer-review

17 Citations (Scopus)

Abstract

Immunoliposomes were prepared by using biosynthetically lipid-tagged anti-2-phenyloxazolone single-chain antibody. Carboxyfluorescein as a fluorescent marker was encapsulated in the immunoliposomes. Some conditions for fluoroimmunoassay using the immunoliposomes were optimized by binding assays with hapten-coated microtiter wells. A competitive fluoroimmunoassay for the caproic acid conjugate of 2-phenyloxazolone as a model antigen was performed with the immunoliposomes. In the optimized assay conditions, antigen could be determined in the concentration range from 10^-7 to 10^-9 M.

Original language	English
Pages (from-to)	1295-1298
Journal	Analytical Chemistry
Volume	69
Issue number	7
DOIs	https://doi.org/10.1021/ac961162+
Publication status	Published - 1997
MoE publication type	A1 Journal article-refereed

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title = "A fluoroimmunoassay based on immunoliposomes containing genetically engineered lipid-tagged antibody",

abstract = "Immunoliposomes were prepared by using biosynthetically lipid-tagged anti-2-phenyloxazolone single-chain antibody. Carboxyfluorescein as a fluorescent marker was encapsulated in the immunoliposomes. Some conditions for fluoroimmunoassay using the immunoliposomes were optimized by binding assays with hapten-coated microtiter wells. A competitive fluoroimmunoassay for the caproic acid conjugate of 2-phenyloxazolone as a model antigen was performed with the immunoliposomes. In the optimized assay conditions, antigen could be determined in the concentration range from 10-7 to 10-9 M. ",

author = "Eiry Kobatake and Hiroyuki Sasakura and Tetsuya Haruyama and Marja-Leena Laukkanen and Kari Kein{\"a}nen and Masuo Aizawa",

year = "1997",

doi = "10.1021/ac961162+",

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journal = "Analytical Chemistry",

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T1 - A fluoroimmunoassay based on immunoliposomes containing genetically engineered lipid-tagged antibody

AU - Kobatake, Eiry

AU - Sasakura, Hiroyuki

AU - Haruyama, Tetsuya

AU - Laukkanen, Marja-Leena

AU - Keinänen, Kari

AU - Aizawa, Masuo

PY - 1997

Y1 - 1997

N2 - Immunoliposomes were prepared by using biosynthetically lipid-tagged anti-2-phenyloxazolone single-chain antibody. Carboxyfluorescein as a fluorescent marker was encapsulated in the immunoliposomes. Some conditions for fluoroimmunoassay using the immunoliposomes were optimized by binding assays with hapten-coated microtiter wells. A competitive fluoroimmunoassay for the caproic acid conjugate of 2-phenyloxazolone as a model antigen was performed with the immunoliposomes. In the optimized assay conditions, antigen could be determined in the concentration range from 10-7 to 10-9 M.

AB - Immunoliposomes were prepared by using biosynthetically lipid-tagged anti-2-phenyloxazolone single-chain antibody. Carboxyfluorescein as a fluorescent marker was encapsulated in the immunoliposomes. Some conditions for fluoroimmunoassay using the immunoliposomes were optimized by binding assays with hapten-coated microtiter wells. A competitive fluoroimmunoassay for the caproic acid conjugate of 2-phenyloxazolone as a model antigen was performed with the immunoliposomes. In the optimized assay conditions, antigen could be determined in the concentration range from 10-7 to 10-9 M.

U2 - 10.1021/ac961162+

DO - 10.1021/ac961162+

M3 - Article

SN - 0003-2700

VL - 69

SP - 1295

EP - 1298

JO - Analytical Chemistry

JF - Analytical Chemistry

IS - 7

ER -

A fluoroimmunoassay based on immunoliposomes containing genetically engineered lipid-tagged antibody

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