A functional genomics approach toward the understanding of plant secondary metabolism: I. Transcriptomics and high-throughput functional analysis of genes in plant cells

Alain Goossens, Valerie De Sutter, Freia Lammertyn, Suvi T. Häkkinen, Heiko Rischer, Kirsi-Marja Oksman-Caldentey, Dirk Inze

Research output: Contribution to conferenceConference articleScientific

Abstract

Genetic knowledge on biosynthetic pathways and the regulation thereof is of crucial importance to bypass the low-product yield of various secondary metabolites in plant cells. To facilitate gene discovery in plant secondary metabolism, we have developed a comprehensive profiling approach that is based on functional genomics and can be applied on any plant system, without a need for prior sequence knowledge. Thus far, we have monitored jasmonate-induced changes on the transcript and alkaloid profiles of tobacco BY-2 and Catharanthus roseus cell cultures. Using cDNA-AFLP based transcript profiling, an inventory of hundreds of genes, potentially involved in plant secondary metabolism, has been built. A technology platform, especially driven towards the exploitation of plant cell suspension cultures, for high-throughput isolation and functional analysis of these genes has been established. This platform will allow creating a novel toolbox for metabolic engineering of plant cells. At present, more than 50 tobacco BY-2 full-length open reading frames have been isolated and are currently subjected to functional analysis in transgenic tobacco, Hyoscyamus muticus and C. roseus cell or hairy root lines.
Original languageEnglish
Publication statusPublished - 2004
MoE publication typeNot Eligible
EventInternational Congress on Natural Products Research - Phoenix, United States
Duration: 31 Jul 20044 Aug 2004

Conference

ConferenceInternational Congress on Natural Products Research
CountryUnited States
CityPhoenix
Period31/07/044/08/04

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Goossens, A., De Sutter, V., Lammertyn, F., Häkkinen, S. T., Rischer, H., Oksman-Caldentey, K-M., & Inze, D. (2004). A functional genomics approach toward the understanding of plant secondary metabolism: I. Transcriptomics and high-throughput functional analysis of genes in plant cells. Paper presented at International Congress on Natural Products Research, Phoenix, United States.