Abstract
A novel technology platform, developed by us, facilitates the discovery
of differentially expressed genes in any plant system without pre-existing
sequence knowledge and allows therefore the isolation of specific full-length
cDNAs which can be further characterized by:
A. Functional analysis by homologous expression
In our tobacco BY-2 model system the overexpression of 22 genes resulted in
three lines exhibiting an altered alkaloid accumulation pattern compared to
controls. Targeted metabolite analysis is carried out by HPLC-PDA.
B. Combinatorial biochemistry by heterologous expression
The genes derived from the tobacco transcriptional profiling are used for the
transformation of other species, too. One of the tested genes for example
influences the tropane alkaloid biosynthesis, which is partly the same as for
nicotine alkaloids, in Hyoscyamus muticus hairy roots. The effects on the
completely different terpenoid indole alkaloids in Catharanthus roseus hairy
roots are also investigated. GC- and LC-systems are used for the analysis of
alkaloids.
Original language | English |
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Publication status | Published - 2004 |
MoE publication type | Not Eligible |
Event | International Congress on Natural Products Research - Phoenix, United States Duration: 31 Jul 2004 → 4 Aug 2004 |
Conference
Conference | International Congress on Natural Products Research |
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Country/Territory | United States |
City | Phoenix |
Period | 31/07/04 → 4/08/04 |