A functional genomics approach toward the understanding of plant secondary metabolism

II. Functional analysis and combinatorial biochemistry in different expression systems

Heiko Rischer, Suvi T. Häkkinen, Tuulikki Seppänen-Laakso, Preeti Oza, Alain Goossens, Valerie De Sutter, Dirk Inze, Kirsi-Marja Oksman-Caldentey

Research output: Contribution to conferenceConference articleScientific

Abstract

A novel technology platform, developed by us, facilitates the discovery of differentially expressed genes in any plant system without pre-existing sequence knowledge and allows therefore the isolation of specific full-length cDNAs which can be further characterized by: A. Functional analysis by homologous expression In our tobacco BY-2 model system the overexpression of 22 genes resulted in three lines exhibiting an altered alkaloid accumulation pattern compared to controls. Targeted metabolite analysis is carried out by HPLC-PDA. B. Combinatorial biochemistry by heterologous expression The genes derived from the tobacco transcriptional profiling are used for the transformation of other species, too. One of the tested genes for example influences the tropane alkaloid biosynthesis, which is partly the same as for nicotine alkaloids, in Hyoscyamus muticus hairy roots. The effects on the completely different terpenoid indole alkaloids in Catharanthus roseus hairy roots are also investigated. GC- and LC-systems are used for the analysis of alkaloids.
Original languageEnglish
Publication statusPublished - 2004
MoE publication typeNot Eligible
EventInternational Congress on Natural Products Research - Phoenix, United States
Duration: 31 Jul 20044 Aug 2004

Conference

ConferenceInternational Congress on Natural Products Research
CountryUnited States
CityPhoenix
Period31/07/044/08/04

Fingerprint

biochemistry
genomics
alkaloids
metabolism
tobacco
genes
Hyoscyamus muticus
tropane alkaloids
indole alkaloids
Catharanthus roseus
nicotine
terpenoids
biosynthesis
metabolites

Cite this

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title = "A functional genomics approach toward the understanding of plant secondary metabolism: II. Functional analysis and combinatorial biochemistry in different expression systems",
abstract = "A novel technology platform, developed by us, facilitates the discovery of differentially expressed genes in any plant system without pre-existing sequence knowledge and allows therefore the isolation of specific full-length cDNAs which can be further characterized by: A. Functional analysis by homologous expression In our tobacco BY-2 model system the overexpression of 22 genes resulted in three lines exhibiting an altered alkaloid accumulation pattern compared to controls. Targeted metabolite analysis is carried out by HPLC-PDA. B. Combinatorial biochemistry by heterologous expression The genes derived from the tobacco transcriptional profiling are used for the transformation of other species, too. One of the tested genes for example influences the tropane alkaloid biosynthesis, which is partly the same as for nicotine alkaloids, in Hyoscyamus muticus hairy roots. The effects on the completely different terpenoid indole alkaloids in Catharanthus roseus hairy roots are also investigated. GC- and LC-systems are used for the analysis of alkaloids.",
author = "Heiko Rischer and H{\"a}kkinen, {Suvi T.} and Tuulikki Sepp{\"a}nen-Laakso and Preeti Oza and Alain Goossens and {De Sutter}, Valerie and Dirk Inze and Kirsi-Marja Oksman-Caldentey",
year = "2004",
language = "English",
note = "International Congress on Natural Products Research ; Conference date: 31-07-2004 Through 04-08-2004",

}

Rischer, H, Häkkinen, ST, Seppänen-Laakso, T, Oza, P, Goossens, A, De Sutter, V, Inze, D & Oksman-Caldentey, K-M 2004, 'A functional genomics approach toward the understanding of plant secondary metabolism: II. Functional analysis and combinatorial biochemistry in different expression systems' Paper presented at International Congress on Natural Products Research, Phoenix, United States, 31/07/04 - 4/08/04, .

A functional genomics approach toward the understanding of plant secondary metabolism : II. Functional analysis and combinatorial biochemistry in different expression systems. / Rischer, Heiko; Häkkinen, Suvi T.; Seppänen-Laakso, Tuulikki; Oza, Preeti; Goossens, Alain; De Sutter, Valerie; Inze, Dirk; Oksman-Caldentey, Kirsi-Marja.

2004. Paper presented at International Congress on Natural Products Research, Phoenix, United States.

Research output: Contribution to conferenceConference articleScientific

TY - CONF

T1 - A functional genomics approach toward the understanding of plant secondary metabolism

T2 - II. Functional analysis and combinatorial biochemistry in different expression systems

AU - Rischer, Heiko

AU - Häkkinen, Suvi T.

AU - Seppänen-Laakso, Tuulikki

AU - Oza, Preeti

AU - Goossens, Alain

AU - De Sutter, Valerie

AU - Inze, Dirk

AU - Oksman-Caldentey, Kirsi-Marja

PY - 2004

Y1 - 2004

N2 - A novel technology platform, developed by us, facilitates the discovery of differentially expressed genes in any plant system without pre-existing sequence knowledge and allows therefore the isolation of specific full-length cDNAs which can be further characterized by: A. Functional analysis by homologous expression In our tobacco BY-2 model system the overexpression of 22 genes resulted in three lines exhibiting an altered alkaloid accumulation pattern compared to controls. Targeted metabolite analysis is carried out by HPLC-PDA. B. Combinatorial biochemistry by heterologous expression The genes derived from the tobacco transcriptional profiling are used for the transformation of other species, too. One of the tested genes for example influences the tropane alkaloid biosynthesis, which is partly the same as for nicotine alkaloids, in Hyoscyamus muticus hairy roots. The effects on the completely different terpenoid indole alkaloids in Catharanthus roseus hairy roots are also investigated. GC- and LC-systems are used for the analysis of alkaloids.

AB - A novel technology platform, developed by us, facilitates the discovery of differentially expressed genes in any plant system without pre-existing sequence knowledge and allows therefore the isolation of specific full-length cDNAs which can be further characterized by: A. Functional analysis by homologous expression In our tobacco BY-2 model system the overexpression of 22 genes resulted in three lines exhibiting an altered alkaloid accumulation pattern compared to controls. Targeted metabolite analysis is carried out by HPLC-PDA. B. Combinatorial biochemistry by heterologous expression The genes derived from the tobacco transcriptional profiling are used for the transformation of other species, too. One of the tested genes for example influences the tropane alkaloid biosynthesis, which is partly the same as for nicotine alkaloids, in Hyoscyamus muticus hairy roots. The effects on the completely different terpenoid indole alkaloids in Catharanthus roseus hairy roots are also investigated. GC- and LC-systems are used for the analysis of alkaloids.

M3 - Conference article

ER -