A functional genomics approach toward the understanding of secondary metabolism in plant cells

Alain Goossens, Suvi T. Häkkinen, Into Laakso, Tuulikki Seppänen-Laakso, Stefania Biondi, Valerie De Sutter, Freya Lammertyn, Anna-Maria Nuutila, Hans Söderlund, Marc Zabeau, Dirk Inze (Corresponding Author), Kirsi-Marja Oksman-Caldentey

Research output: Contribution to journalArticle in a proceedings journalScientificpeer-review

297 Citations (Scopus)

Abstract

Despite the tremendous importance of secondary metabolites for humans as for the plant itself, plant secondary metabolism remains poorly characterized. Here, we present an experimental approach, based on functional genomics, to facilitate gene discovery in plant secondary metabolism. Targeted metabolite analysis was combined with cDNA-amplified fragment length polymorphism-based transcript profiling of jasmonate-elicited tobacco Bright yellow 2 cells. Transcriptome analysis suggested an extensive jasmonate-mediated genetic reprogramming of metabolism, which correlated well with the observed shifts in the biosynthesis of the metabolites investigated. This method, which in addition to transcriptome data also generates gene tags, in the future might lead to the creation of novel tools for metabolic engineering of medicinal plant systems in general.
Original languageEnglish
Pages (from-to)8595-8600
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume100
Issue number14
DOIs
Publication statusPublished - 2003
MoE publication typeA4 Article in a conference publication

Keywords

  • metabolites
  • plant metabolites
  • secondary metabolites
  • metabolomics

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