A functional genomics approach toward the understanding of secondary metabolism in plant cells

Alain Goossens, Suvi T. Häkkinen, Into Laakso, Tuulikki Seppänen-Laakso, Stefania Biondi, Valerie De Sutter, Freya Lammertyn, Anna-Maria Nuutila, Hans Söderlund, Marc Zabeau, Dirk Inze (Corresponding Author), Kirsi-Marja Oksman-Caldentey

Research output: Contribution to journalArticle in a proceedings journalScientificpeer-review

287 Citations (Scopus)

Abstract

Despite the tremendous importance of secondary metabolites for humans as for the plant itself, plant secondary metabolism remains poorly characterized. Here, we present an experimental approach, based on functional genomics, to facilitate gene discovery in plant secondary metabolism. Targeted metabolite analysis was combined with cDNA-amplified fragment length polymorphism-based transcript profiling of jasmonate-elicited tobacco Bright yellow 2 cells. Transcriptome analysis suggested an extensive jasmonate-mediated genetic reprogramming of metabolism, which correlated well with the observed shifts in the biosynthesis of the metabolites investigated. This method, which in addition to transcriptome data also generates gene tags, in the future might lead to the creation of novel tools for metabolic engineering of medicinal plant systems in general.
Original languageEnglish
Pages (from-to)8595-8600
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume100
Issue number14
DOIs
Publication statusPublished - 2003
MoE publication typeA4 Article in a conference publication

Fingerprint

genomics
metabolism
metabolites
metabolic engineering
cells
transcriptomics
transcriptome
secondary metabolites
amplified fragment length polymorphism
medicinal plants
tobacco
genes
biosynthesis
methodology

Keywords

  • metabolites
  • plant metabolites
  • secondary metabolites
  • metabolomics

Cite this

Goossens, Alain ; Häkkinen, Suvi T. ; Laakso, Into ; Seppänen-Laakso, Tuulikki ; Biondi, Stefania ; De Sutter, Valerie ; Lammertyn, Freya ; Nuutila, Anna-Maria ; Söderlund, Hans ; Zabeau, Marc ; Inze, Dirk ; Oksman-Caldentey, Kirsi-Marja. / A functional genomics approach toward the understanding of secondary metabolism in plant cells. In: Proceedings of the National Academy of Sciences of the United States of America. 2003 ; Vol. 100, No. 14. pp. 8595-8600.
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A functional genomics approach toward the understanding of secondary metabolism in plant cells. / Goossens, Alain; Häkkinen, Suvi T.; Laakso, Into; Seppänen-Laakso, Tuulikki; Biondi, Stefania; De Sutter, Valerie; Lammertyn, Freya; Nuutila, Anna-Maria; Söderlund, Hans; Zabeau, Marc; Inze, Dirk (Corresponding Author); Oksman-Caldentey, Kirsi-Marja.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, No. 14, 2003, p. 8595-8600.

Research output: Contribution to journalArticle in a proceedings journalScientificpeer-review

TY - JOUR

T1 - A functional genomics approach toward the understanding of secondary metabolism in plant cells

AU - Goossens, Alain

AU - Häkkinen, Suvi T.

AU - Laakso, Into

AU - Seppänen-Laakso, Tuulikki

AU - Biondi, Stefania

AU - De Sutter, Valerie

AU - Lammertyn, Freya

AU - Nuutila, Anna-Maria

AU - Söderlund, Hans

AU - Zabeau, Marc

AU - Inze, Dirk

AU - Oksman-Caldentey, Kirsi-Marja

PY - 2003

Y1 - 2003

N2 - Despite the tremendous importance of secondary metabolites for humans as for the plant itself, plant secondary metabolism remains poorly characterized. Here, we present an experimental approach, based on functional genomics, to facilitate gene discovery in plant secondary metabolism. Targeted metabolite analysis was combined with cDNA-amplified fragment length polymorphism-based transcript profiling of jasmonate-elicited tobacco Bright yellow 2 cells. Transcriptome analysis suggested an extensive jasmonate-mediated genetic reprogramming of metabolism, which correlated well with the observed shifts in the biosynthesis of the metabolites investigated. This method, which in addition to transcriptome data also generates gene tags, in the future might lead to the creation of novel tools for metabolic engineering of medicinal plant systems in general.

AB - Despite the tremendous importance of secondary metabolites for humans as for the plant itself, plant secondary metabolism remains poorly characterized. Here, we present an experimental approach, based on functional genomics, to facilitate gene discovery in plant secondary metabolism. Targeted metabolite analysis was combined with cDNA-amplified fragment length polymorphism-based transcript profiling of jasmonate-elicited tobacco Bright yellow 2 cells. Transcriptome analysis suggested an extensive jasmonate-mediated genetic reprogramming of metabolism, which correlated well with the observed shifts in the biosynthesis of the metabolites investigated. This method, which in addition to transcriptome data also generates gene tags, in the future might lead to the creation of novel tools for metabolic engineering of medicinal plant systems in general.

KW - metabolites

KW - plant metabolites

KW - secondary metabolites

KW - metabolomics

U2 - 10.1073/pnas.1032967100

DO - 10.1073/pnas.1032967100

M3 - Article in a proceedings journal

VL - 100

SP - 8595

EP - 8600

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 14

ER -