A his-tagged Melanocarpus albomyces laccase and its electrochemistry upon immobilisation on NTA-modified electrodes and in conducting polymer films

M. Sosna, Harry Boer, P.N. Bartlett (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

9 Citations (Scopus)

Abstract

The article describes the construction, immobilisation and electrochemistry of histidine tagged laccase from Melanocarpus albomyces. A facile method of functionalisation of glassy carbon electrodes with nitrilotriacetic acid (NTA) using diazonium grafting and solid state chemistry is described. NTA‐modified electrodes are shown to bind laccase which reduces oxygen at neutral pH in the presence of soluble redox mediator. Laccase‐modified electrodes are also prepared by enzyme immobilisation within poly(aniline)/poly(vinylsulfonate) films. The polymer is found to efficiently retain the enzyme as well as provide direct electrical contact between the electrode and the enzyme active centre. Cyclic voltammetry reveals the direct electron transfer to the enzyme is dependent on the redox state of the polymer film.
Original languageEnglish
Pages (from-to)2225-2231
Number of pages6
JournalChemPhysChem
Volume14
Issue number10
DOIs
Publication statusPublished - 2013
MoE publication typeA1 Journal article-refereed

Fingerprint

Nitrilotriacetic Acid
Laccase
Conducting polymers
conducting polymers
Electrochemistry
electrochemistry
immobilization
Polymer films
enzymes
Electrodes
acids
electrodes
Enzymes
Enzyme immobilization
histidine
glassy carbon
Glassy carbon
polymers
aniline
Histidine

Keywords

  • biofuel cells
  • direct electron transfer
  • laccase
  • modified electrodes
  • polyaniline

Cite this

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title = "A his-tagged Melanocarpus albomyces laccase and its electrochemistry upon immobilisation on NTA-modified electrodes and in conducting polymer films",
abstract = "The article describes the construction, immobilisation and electrochemistry of histidine tagged laccase from Melanocarpus albomyces. A facile method of functionalisation of glassy carbon electrodes with nitrilotriacetic acid (NTA) using diazonium grafting and solid state chemistry is described. NTA‐modified electrodes are shown to bind laccase which reduces oxygen at neutral pH in the presence of soluble redox mediator. Laccase‐modified electrodes are also prepared by enzyme immobilisation within poly(aniline)/poly(vinylsulfonate) films. The polymer is found to efficiently retain the enzyme as well as provide direct electrical contact between the electrode and the enzyme active centre. Cyclic voltammetry reveals the direct electron transfer to the enzyme is dependent on the redox state of the polymer film.",
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A his-tagged Melanocarpus albomyces laccase and its electrochemistry upon immobilisation on NTA-modified electrodes and in conducting polymer films. / Sosna, M.; Boer, Harry; Bartlett, P.N. (Corresponding Author).

In: ChemPhysChem, Vol. 14, No. 10, 2013, p. 2225-2231.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - A his-tagged Melanocarpus albomyces laccase and its electrochemistry upon immobilisation on NTA-modified electrodes and in conducting polymer films

AU - Sosna, M.

AU - Boer, Harry

AU - Bartlett, P.N.

PY - 2013

Y1 - 2013

N2 - The article describes the construction, immobilisation and electrochemistry of histidine tagged laccase from Melanocarpus albomyces. A facile method of functionalisation of glassy carbon electrodes with nitrilotriacetic acid (NTA) using diazonium grafting and solid state chemistry is described. NTA‐modified electrodes are shown to bind laccase which reduces oxygen at neutral pH in the presence of soluble redox mediator. Laccase‐modified electrodes are also prepared by enzyme immobilisation within poly(aniline)/poly(vinylsulfonate) films. The polymer is found to efficiently retain the enzyme as well as provide direct electrical contact between the electrode and the enzyme active centre. Cyclic voltammetry reveals the direct electron transfer to the enzyme is dependent on the redox state of the polymer film.

AB - The article describes the construction, immobilisation and electrochemistry of histidine tagged laccase from Melanocarpus albomyces. A facile method of functionalisation of glassy carbon electrodes with nitrilotriacetic acid (NTA) using diazonium grafting and solid state chemistry is described. NTA‐modified electrodes are shown to bind laccase which reduces oxygen at neutral pH in the presence of soluble redox mediator. Laccase‐modified electrodes are also prepared by enzyme immobilisation within poly(aniline)/poly(vinylsulfonate) films. The polymer is found to efficiently retain the enzyme as well as provide direct electrical contact between the electrode and the enzyme active centre. Cyclic voltammetry reveals the direct electron transfer to the enzyme is dependent on the redox state of the polymer film.

KW - biofuel cells

KW - direct electron transfer

KW - laccase

KW - modified electrodes

KW - polyaniline

U2 - 10.1002/cphc.201300340

DO - 10.1002/cphc.201300340

M3 - Article

VL - 14

SP - 2225

EP - 2231

JO - ChemPhysChem

JF - ChemPhysChem

SN - 1439-4235

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