A his-tagged Melanocarpus albomyces laccase and its electrochemistry upon immobilisation on NTA-modified electrodes and in conducting polymer films

M. Sosna, Harry Boer, P.N. Bartlett (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

9 Citations (Scopus)


The article describes the construction, immobilisation and electrochemistry of histidine tagged laccase from Melanocarpus albomyces. A facile method of functionalisation of glassy carbon electrodes with nitrilotriacetic acid (NTA) using diazonium grafting and solid state chemistry is described. NTA‐modified electrodes are shown to bind laccase which reduces oxygen at neutral pH in the presence of soluble redox mediator. Laccase‐modified electrodes are also prepared by enzyme immobilisation within poly(aniline)/poly(vinylsulfonate) films. The polymer is found to efficiently retain the enzyme as well as provide direct electrical contact between the electrode and the enzyme active centre. Cyclic voltammetry reveals the direct electron transfer to the enzyme is dependent on the redox state of the polymer film.
Original languageEnglish
Pages (from-to)2225-2231
Number of pages6
Issue number10
Publication statusPublished - 2013
MoE publication typeA1 Journal article-refereed



  • biofuel cells
  • direct electron transfer
  • laccase
  • modified electrodes
  • polyaniline

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