A lignin peroxidase-encoding cDNA from the white-rot fungus Phlebia radiata: Characterization and expression in Trichoderma reesei

Markku Saloheimo (Corresponding Author), Victoria Barajas, Marja-Leena Niku-Paavola, Jonathan Knowles

Research output: Contribution to journalArticleScientificpeer-review

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Abstract

The nucleotide sequence of a cDNA coding for a lignin peroxidase (Lgp) of the white-rot fungus, Phlebia radiata has been determined. By amino acid (aa) sequencing, it has been shown that the protein product of this gene is the LIII Lgp of Pb. radiata.
The isolated gene and the putative aa sequence are about 60% homologous to published Lgp sequences from the fungus, Phanerochaete chrysosporium. The aa thought to be involved in the catalysis of LIII are revealed by comparison with the yeast cytochrome c peroxidase. The P. radiata Lgp-encoding gene (lgp3) was expressed in the fungus, Trichoderma reesei, under the cellobiohydrolase-encoding cbh1 gene promoter. Lgp3 mRNA was produced by the T. reesei transformants. No Lgp protein, however, could be detected.
Original languageEnglish
Pages (from-to)343-351
JournalGene
Volume85
Issue number2
DOIs
Publication statusPublished - 1989
MoE publication typeA1 Journal article-refereed

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Trichoderma
Fungi
Complementary DNA
Cytochrome-c Peroxidase
Cellulose 1,4-beta-Cellobiosidase
Phanerochaete
Genes
Protein Sequence Analysis
Catalysis
Amino Acid Sequence
Proteins
Yeasts
lignin peroxidase
Amino Acids
Messenger RNA

Cite this

Saloheimo, Markku ; Barajas, Victoria ; Niku-Paavola, Marja-Leena ; Knowles, Jonathan. / A lignin peroxidase-encoding cDNA from the white-rot fungus Phlebia radiata : Characterization and expression in Trichoderma reesei. In: Gene. 1989 ; Vol. 85, No. 2. pp. 343-351.
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abstract = "The nucleotide sequence of a cDNA coding for a lignin peroxidase (Lgp) of the white-rot fungus, Phlebia radiata has been determined. By amino acid (aa) sequencing, it has been shown that the protein product of this gene is the LIII Lgp of Pb. radiata. The isolated gene and the putative aa sequence are about 60{\%} homologous to published Lgp sequences from the fungus, Phanerochaete chrysosporium. The aa thought to be involved in the catalysis of LIII are revealed by comparison with the yeast cytochrome c peroxidase. The P. radiata Lgp-encoding gene (lgp3) was expressed in the fungus, Trichoderma reesei, under the cellobiohydrolase-encoding cbh1 gene promoter. Lgp3 mRNA was produced by the T. reesei transformants. No Lgp protein, however, could be detected.",
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A lignin peroxidase-encoding cDNA from the white-rot fungus Phlebia radiata : Characterization and expression in Trichoderma reesei. / Saloheimo, Markku (Corresponding Author); Barajas, Victoria; Niku-Paavola, Marja-Leena; Knowles, Jonathan.

In: Gene, Vol. 85, No. 2, 1989, p. 343-351.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - A lignin peroxidase-encoding cDNA from the white-rot fungus Phlebia radiata

T2 - Characterization and expression in Trichoderma reesei

AU - Saloheimo, Markku

AU - Barajas, Victoria

AU - Niku-Paavola, Marja-Leena

AU - Knowles, Jonathan

PY - 1989

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N2 - The nucleotide sequence of a cDNA coding for a lignin peroxidase (Lgp) of the white-rot fungus, Phlebia radiata has been determined. By amino acid (aa) sequencing, it has been shown that the protein product of this gene is the LIII Lgp of Pb. radiata. The isolated gene and the putative aa sequence are about 60% homologous to published Lgp sequences from the fungus, Phanerochaete chrysosporium. The aa thought to be involved in the catalysis of LIII are revealed by comparison with the yeast cytochrome c peroxidase. The P. radiata Lgp-encoding gene (lgp3) was expressed in the fungus, Trichoderma reesei, under the cellobiohydrolase-encoding cbh1 gene promoter. Lgp3 mRNA was produced by the T. reesei transformants. No Lgp protein, however, could be detected.

AB - The nucleotide sequence of a cDNA coding for a lignin peroxidase (Lgp) of the white-rot fungus, Phlebia radiata has been determined. By amino acid (aa) sequencing, it has been shown that the protein product of this gene is the LIII Lgp of Pb. radiata. The isolated gene and the putative aa sequence are about 60% homologous to published Lgp sequences from the fungus, Phanerochaete chrysosporium. The aa thought to be involved in the catalysis of LIII are revealed by comparison with the yeast cytochrome c peroxidase. The P. radiata Lgp-encoding gene (lgp3) was expressed in the fungus, Trichoderma reesei, under the cellobiohydrolase-encoding cbh1 gene promoter. Lgp3 mRNA was produced by the T. reesei transformants. No Lgp protein, however, could be detected.

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DO - 10.1016/0378-1119(89)90427-7

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JO - Gene

JF - Gene

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