TY - JOUR
T1 - A low-cost paper-based platform for fast and reliable screening of cellular interactions with materials
AU - Rosqvist, Emil
AU - Niemelä, Erik
AU - Frisk, Johan
AU - Öblom, Heidi
AU - Koppolu, Rajesh
AU - Abdelkader, Hend
AU - Véliz, Diosangeles Soto
AU - Mennillo, Marco
AU - Venu, Arun Poonthuruthikudy
AU - Ihalainen, Petri
AU - Aubert, Melanie
AU - Sandler, Niklas
AU - Wilén, Carl-Erik
AU - Toivakka, Martti
AU - Eriksson, John E.
AU - Österbacka, Ronald
AU - Peltonen, Jouko
N1 - Funding Information:
All project members and laboratories are cordially thanked for the communal efforts, in particular Ruut Kummala and Markus Pesonen. This work was financed by the Åbo Akademi University through the Centre of Excellence project – Functional Materials at Biological Interfaces as well as personal grants, Tor, Joe and Pentti Borgs Minnesfond and Svenska Kulturfonden.
Publisher Copyright:
© 2020 The Royal Society of Chemistry.
PY - 2020
Y1 - 2020
N2 - A paper-based platform was developed and tested for studies on basic cell culture, material biocompatibility, and activity of pharmaceuticals in order to provide a reliable, robust and low-cost cell study platform. It is based upon a paper or paperboard support, with a nanostructured latex coating to provide an enhanced cell growth and sufficient barrier properties. Wetting is limited to regions of interest using a flexographically printed hydrophobic polydimethylsiloxane layer with circular non-print areas. The nanostructured coating can be substituted for another coating of interest, or the regions of interest functionalized with a material to be studied. The platform is fully up-scalable, being produced with roll-to-roll rod coating, flexographic and inkjet printing methods. Results show that the platform efficiency is comparable to multi-well plates in colorimetric assays in three separate studies: a cell culture study, a biocompatibility study, and a drug screening study. The color intensity is quantified by using a common office scanner or an imaging device and the data is analyzed by a custom computer software without the need for expensive screening or analysis equipment.
AB - A paper-based platform was developed and tested for studies on basic cell culture, material biocompatibility, and activity of pharmaceuticals in order to provide a reliable, robust and low-cost cell study platform. It is based upon a paper or paperboard support, with a nanostructured latex coating to provide an enhanced cell growth and sufficient barrier properties. Wetting is limited to regions of interest using a flexographically printed hydrophobic polydimethylsiloxane layer with circular non-print areas. The nanostructured coating can be substituted for another coating of interest, or the regions of interest functionalized with a material to be studied. The platform is fully up-scalable, being produced with roll-to-roll rod coating, flexographic and inkjet printing methods. Results show that the platform efficiency is comparable to multi-well plates in colorimetric assays in three separate studies: a cell culture study, a biocompatibility study, and a drug screening study. The color intensity is quantified by using a common office scanner or an imaging device and the data is analyzed by a custom computer software without the need for expensive screening or analysis equipment.
UR - https://www.scopus.com/pages/publications/85079320412
U2 - 10.1039/C9TB01958H
DO - 10.1039/C9TB01958H
M3 - Article
SN - 2050-750X
VL - 8
SP - 1146
EP - 1156
JO - Journal of Materials Chemistry B
JF - Journal of Materials Chemistry B
IS - 6
ER -