A modified Kelsey‐Sykes method for testing disinfectants with 2,3,5‐triphenyltetrazolium chloride reduction as an indicator of bacterial growth

Tiina Mattila

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14 Citations (Scopus)

Abstract

Different dilutions of chlorhexidine gluconate were tested by the Kelsey‐Sykes procedure. The method was further modified on microtitration plates using 2,3,5‐triphenyltetrazolium chloride (TTC) reduction as an indicator of bacterial growth.
There was a good correlation with the results based on TTC reduction and the conventional method based on turbidity changes caused by bacterial growth.
Furthermore, the modified method using TTC reduction is more rapid and can be read by the naked eye because of the red colour.
Original languageEnglish
Pages (from-to)551-554
JournalJournal of Applied Bacteriology
Volume62
Issue number6
DOIs
Publication statusPublished - 1987
MoE publication typeA1 Journal article-refereed

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Disinfectants
Chlorides
Growth
Color

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title = "A modified Kelsey‐Sykes method for testing disinfectants with 2,3,5‐triphenyltetrazolium chloride reduction as an indicator of bacterial growth",
abstract = "Different dilutions of chlorhexidine gluconate were tested by the Kelsey‐Sykes procedure. The method was further modified on microtitration plates using 2,3,5‐triphenyltetrazolium chloride (TTC) reduction as an indicator of bacterial growth. There was a good correlation with the results based on TTC reduction and the conventional method based on turbidity changes caused by bacterial growth. Furthermore, the modified method using TTC reduction is more rapid and can be read by the naked eye because of the red colour.",
author = "Tiina Mattila",
year = "1987",
doi = "10.1111/j.1365-2672.1987.tb02688.x",
language = "English",
volume = "62",
pages = "551--554",
journal = "Journal of Applied Microbiology",
issn = "1364-5072",
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A modified Kelsey‐Sykes method for testing disinfectants with 2,3,5‐triphenyltetrazolium chloride reduction as an indicator of bacterial growth. / Mattila, Tiina.

In: Journal of Applied Bacteriology, Vol. 62, No. 6, 1987, p. 551-554.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - A modified Kelsey‐Sykes method for testing disinfectants with 2,3,5‐triphenyltetrazolium chloride reduction as an indicator of bacterial growth

AU - Mattila, Tiina

PY - 1987

Y1 - 1987

N2 - Different dilutions of chlorhexidine gluconate were tested by the Kelsey‐Sykes procedure. The method was further modified on microtitration plates using 2,3,5‐triphenyltetrazolium chloride (TTC) reduction as an indicator of bacterial growth. There was a good correlation with the results based on TTC reduction and the conventional method based on turbidity changes caused by bacterial growth. Furthermore, the modified method using TTC reduction is more rapid and can be read by the naked eye because of the red colour.

AB - Different dilutions of chlorhexidine gluconate were tested by the Kelsey‐Sykes procedure. The method was further modified on microtitration plates using 2,3,5‐triphenyltetrazolium chloride (TTC) reduction as an indicator of bacterial growth. There was a good correlation with the results based on TTC reduction and the conventional method based on turbidity changes caused by bacterial growth. Furthermore, the modified method using TTC reduction is more rapid and can be read by the naked eye because of the red colour.

U2 - 10.1111/j.1365-2672.1987.tb02688.x

DO - 10.1111/j.1365-2672.1987.tb02688.x

M3 - Article

VL - 62

SP - 551

EP - 554

JO - Journal of Applied Microbiology

JF - Journal of Applied Microbiology

SN - 1364-5072

IS - 6

ER -