A new appraisal of the endoglucanases of the fungus Trichoderma reesei

Marja-Leena Niku-Paavola, Arja Lappalainen, Tor-Magnus Enari, Martti Nummi

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Abstract

The properties and enzymic activity of endoglucanases (EC 3.2.1.4) of the fungus Trichoderma reesei were studied by means of immunological methods and by using polyglycosidic substrates. Endoglucanases exist in the culture liquid as a series of immunologically related components. The most active endoglucanase component has an Mr of 43 000 and pI value of 4.0. The most abundant components have a value of pI about 5.0, an Mr of 56 000-67 000 and specific activity only one-fifth of that of the pI-4.0 component. During purification and storage the endoglucanases are spontaneously modified; the relative proportion of components having greater Mr values, more alkaline pI values and lower specific activities is increased. The hexose content of the endoglucanase components is 2-7%. Endoglucanases hydrolyse soluble β-1,4 glycans. The enzymes described here differ from endoglucanase preparations described previously in not showing activity towards insoluble substrates. The role of endoglucanases in wood hydrolysis is consequently limited to the stage where wood constituents are already in soluble form.

Original languageEnglish
Pages (from-to)75 - 81
Number of pages7
JournalBiochemical Journal
Volume231
Issue number1
DOIs
Publication statusPublished - 1985
MoE publication typeNot Eligible

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Trichoderma
Cellulase
Fungi
Wood
Hexoses
Substrates
Purification
Polysaccharides
Hydrolysis
Liquids
Enzymes

Cite this

Niku-Paavola, M-L., Lappalainen, A., Enari, T-M., & Nummi, M. (1985). A new appraisal of the endoglucanases of the fungus Trichoderma reesei. Biochemical Journal, 231(1), 75 - 81. https://doi.org/10.1042/bj2310075
Niku-Paavola, Marja-Leena ; Lappalainen, Arja ; Enari, Tor-Magnus ; Nummi, Martti. / A new appraisal of the endoglucanases of the fungus Trichoderma reesei. In: Biochemical Journal. 1985 ; Vol. 231, No. 1. pp. 75 - 81.
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abstract = "The properties and enzymic activity of endoglucanases (EC 3.2.1.4) of the fungus Trichoderma reesei were studied by means of immunological methods and by using polyglycosidic substrates. Endoglucanases exist in the culture liquid as a series of immunologically related components. The most active endoglucanase component has an Mr of 43 000 and pI value of 4.0. The most abundant components have a value of pI about 5.0, an Mr of 56 000-67 000 and specific activity only one-fifth of that of the pI-4.0 component. During purification and storage the endoglucanases are spontaneously modified; the relative proportion of components having greater Mr values, more alkaline pI values and lower specific activities is increased. The hexose content of the endoglucanase components is 2-7{\%}. Endoglucanases hydrolyse soluble β-1,4 glycans. The enzymes described here differ from endoglucanase preparations described previously in not showing activity towards insoluble substrates. The role of endoglucanases in wood hydrolysis is consequently limited to the stage where wood constituents are already in soluble form.",
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Niku-Paavola, M-L, Lappalainen, A, Enari, T-M & Nummi, M 1985, 'A new appraisal of the endoglucanases of the fungus Trichoderma reesei', Biochemical Journal, vol. 231, no. 1, pp. 75 - 81. https://doi.org/10.1042/bj2310075

A new appraisal of the endoglucanases of the fungus Trichoderma reesei. / Niku-Paavola, Marja-Leena; Lappalainen, Arja; Enari, Tor-Magnus; Nummi, Martti.

In: Biochemical Journal, Vol. 231, No. 1, 1985, p. 75 - 81.

Research output: Contribution to journalArticleScientificpeer-review

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