A new method for determining the area of cell walls in rye doughs based on fluorescence microscopy and computer-assisted image analysis

Teija Parkkonen (Corresponding Author), Ritva Heinonen, Karin Autio

Research output: Contribution to journalArticleScientificpeer-review

14 Citations (Scopus)

Abstract

An objective method to quantify visible cell walls in thin sections of rye dough has been developed using light microscopy. This method is based on the detection of Calcofluor-induced fluorescence of mixed-linked (1,3)(1,4)-β-glucan found in cell walls with a fluorescence microscope, and calculating the area of blue fluorescence in the microscope fields. The method was tested on two rheologically different rye doughs; numerous thin sections were cut from different parts of the dough and from two parallel doughs. A clear difference between the doughs was obtained, supporting results published elsewhere. The method was proven to be reproducible and objective by two test persons. The method is applicable to all biological material containing β-glucan and therefore has potential in, for example, the study of the efficiency of nutrient digestion, in enzyme activity research and in the optimization of processing conditions.

Original languageEnglish
Pages (from-to)743 - 747
Number of pages5
JournalLebensmittel-Wissenschaft & Technologie
Volume30
Issue number7
DOIs
Publication statusPublished - 1997
MoE publication typeA1 Journal article-refereed

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Computer-Assisted Image Processing
fluorescence microscopy
Fluorescence Microscopy
dough
rye
Cell Wall
cell walls
image analysis
Fluorescence
fluorescence
glucans
microscopes
methodology
Glucans
light microscopy
Digestion
Microscopy
digestion
Secale
enzyme activity

Cite this

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title = "A new method for determining the area of cell walls in rye doughs based on fluorescence microscopy and computer-assisted image analysis",
abstract = "An objective method to quantify visible cell walls in thin sections of rye dough has been developed using light microscopy. This method is based on the detection of Calcofluor-induced fluorescence of mixed-linked (1,3)(1,4)-β-glucan found in cell walls with a fluorescence microscope, and calculating the area of blue fluorescence in the microscope fields. The method was tested on two rheologically different rye doughs; numerous thin sections were cut from different parts of the dough and from two parallel doughs. A clear difference between the doughs was obtained, supporting results published elsewhere. The method was proven to be reproducible and objective by two test persons. The method is applicable to all biological material containing β-glucan and therefore has potential in, for example, the study of the efficiency of nutrient digestion, in enzyme activity research and in the optimization of processing conditions.",
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A new method for determining the area of cell walls in rye doughs based on fluorescence microscopy and computer-assisted image analysis. / Parkkonen, Teija (Corresponding Author); Heinonen, Ritva; Autio, Karin.

In: Lebensmittel-Wissenschaft & Technologie, Vol. 30, No. 7, 1997, p. 743 - 747.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - A new method for determining the area of cell walls in rye doughs based on fluorescence microscopy and computer-assisted image analysis

AU - Parkkonen, Teija

AU - Heinonen, Ritva

AU - Autio, Karin

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Y1 - 1997

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AB - An objective method to quantify visible cell walls in thin sections of rye dough has been developed using light microscopy. This method is based on the detection of Calcofluor-induced fluorescence of mixed-linked (1,3)(1,4)-β-glucan found in cell walls with a fluorescence microscope, and calculating the area of blue fluorescence in the microscope fields. The method was tested on two rheologically different rye doughs; numerous thin sections were cut from different parts of the dough and from two parallel doughs. A clear difference between the doughs was obtained, supporting results published elsewhere. The method was proven to be reproducible and objective by two test persons. The method is applicable to all biological material containing β-glucan and therefore has potential in, for example, the study of the efficiency of nutrient digestion, in enzyme activity research and in the optimization of processing conditions.

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