A new salt inducible expression system for Lactococcus lactis

Noora Sirén, Kalle Salonen, Matti Leisola, Antti Nyyssölä

    Research output: Contribution to journalArticleScientificpeer-review

    3 Citations (Scopus)

    Abstract

    A new expression system for Lactococcus lactis based on the salt inducible BusA promoter and the BusR repressor gene of L. lactis MG1363 was developed. To achieve salt induction, the expression of BusR was modulated by introducing mutations to its promoter sequence. An activity of 6.0 μkat l−1 of the model enzyme Lactobacillus amylovorus α-amylase was achieved in the bioreactor cultivation. The major advantage of the current expression system is that no additions of inducing agents are needed into bioreactor cultivations.
    Original languageEnglish
    Pages (from-to)132-135
    Number of pages4
    JournalBiochemical Engineering Journal
    Volume48
    Issue number1
    DOIs
    Publication statusPublished - 2009
    MoE publication typeA1 Journal article-refereed

    Keywords

    • bioreactor systems
    • BusR
    • Lactococcus lactis
    • salt induction
    • recombinant proteins
    • expression systems
    • gene expression
    • bioreactor cultivation
    • bioreactors

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