A new salt inducible expression system for Lactococcus lactis

Noora Sirén, Kalle Salonen, Matti Leisola, Antti Nyyssölä

Research output: Contribution to journalArticleScientificpeer-review

3 Citations (Scopus)

Abstract

A new expression system for Lactococcus lactis based on the salt inducible BusA promoter and the BusR repressor gene of L. lactis MG1363 was developed. To achieve salt induction, the expression of BusR was modulated by introducing mutations to its promoter sequence. An activity of 6.0 μkat l−1 of the model enzyme Lactobacillus amylovorus α-amylase was achieved in the bioreactor cultivation. The major advantage of the current expression system is that no additions of inducing agents are needed into bioreactor cultivations.
Original languageEnglish
Pages (from-to)132-135
Number of pages4
JournalBiochemical Engineering Journal
Volume48
Issue number1
DOIs
Publication statusPublished - 2009
MoE publication typeA1 Journal article-refereed

Fingerprint

Lactococcus lactis
Bioreactors
Salts
Lactobacillus acidophilus
Amylases
Enzymes
Genes
Mutation

Keywords

  • bioreactor systems
  • BusR
  • Lactococcus lactis
  • salt induction
  • recombinant proteins
  • expression systems
  • gene expression
  • bioreactor cultivation
  • bioreactors

Cite this

Sirén, Noora ; Salonen, Kalle ; Leisola, Matti ; Nyyssölä, Antti. / A new salt inducible expression system for Lactococcus lactis. In: Biochemical Engineering Journal. 2009 ; Vol. 48, No. 1. pp. 132-135.
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A new salt inducible expression system for Lactococcus lactis. / Sirén, Noora; Salonen, Kalle; Leisola, Matti; Nyyssölä, Antti.

In: Biochemical Engineering Journal, Vol. 48, No. 1, 2009, p. 132-135.

Research output: Contribution to journalArticleScientificpeer-review

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T1 - A new salt inducible expression system for Lactococcus lactis

AU - Sirén, Noora

AU - Salonen, Kalle

AU - Leisola, Matti

AU - Nyyssölä, Antti

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N2 - A new expression system for Lactococcus lactis based on the salt inducible BusA promoter and the BusR repressor gene of L. lactis MG1363 was developed. To achieve salt induction, the expression of BusR was modulated by introducing mutations to its promoter sequence. An activity of 6.0 μkat l−1 of the model enzyme Lactobacillus amylovorus α-amylase was achieved in the bioreactor cultivation. The major advantage of the current expression system is that no additions of inducing agents are needed into bioreactor cultivations.

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KW - bioreactor systems

KW - BusR

KW - Lactococcus lactis

KW - salt induction

KW - recombinant proteins

KW - expression systems

KW - gene expression

KW - bioreactor cultivation

KW - bioreactors

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DO - 10.1016/j.bej.2009.08.003

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