A new technique for the performance evaluation of clean-in-place disinfection of biofilms

L. Willcock, P. Gilbert, J. Holah, Gun Wirtanen, D. G. Allison

Research output: Contribution to journalArticleScientificpeer-review

12 Citations (Scopus)

Abstract

A concentric cylinder reactor (CCR) is described that enables the steady-state kinetics of microbial biofilms to be evaluated under conditions of constant nutrient flow and variable shear-stress. The reactor has been used to evaluate the influence of fluid dynamic shear on the extent and mode of detachment of bacteria from biofilms. Using a food factory isolate of Pseudomonas aeruginosa, a general increase in the overall growth rate and detachment of the biofilms (cfu cm−2 min−1) with time was shown for each biofilm, regardless of the prevailing shear. As the shear rate was increased beyond 0.123 ms−1, populations tended toward a pseudo steady-state. Sudden changes in shear force, however, caused dramatic changes in the productivity of steady-state populations. The CCR provides an effective means of testing disinfectant activity, particularly for clean-in-place situations, and allows for an examination of the residual effects of a cleansing programme on a treated surface for three different chemical classes of disinfectant. Utilisation of the CCR would, therefore, provide enhanced ability to determine the efficacy and efficiency of chemical products for use in sanitation protocols.
Original languageEnglish
Pages (from-to)235 - 241
Number of pages7
JournalJournal of industrial microbiology and biotechnology
Volume25
Issue number5
DOIs
Publication statusPublished - 2000
MoE publication typeA1 Journal article-refereed

Fingerprint

Disinfection
Biofilms
Disinfectants
Food
Sanitation
Hydrodynamics
Fluid dynamics
Shear deformation
Pseudomonas aeruginosa
Population
Nutrients
Industrial plants
Shear stress
Bacteria
Productivity
Kinetics
Testing
Growth

Keywords

  • cleansing
  • biofilm
  • disinfection
  • biocides
  • fluid dynamic shear

Cite this

Willcock, L. ; Gilbert, P. ; Holah, J. ; Wirtanen, Gun ; Allison, D. G. / A new technique for the performance evaluation of clean-in-place disinfection of biofilms. In: Journal of industrial microbiology and biotechnology. 2000 ; Vol. 25, No. 5. pp. 235 - 241.
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abstract = "A concentric cylinder reactor (CCR) is described that enables the steady-state kinetics of microbial biofilms to be evaluated under conditions of constant nutrient flow and variable shear-stress. The reactor has been used to evaluate the influence of fluid dynamic shear on the extent and mode of detachment of bacteria from biofilms. Using a food factory isolate of Pseudomonas aeruginosa, a general increase in the overall growth rate and detachment of the biofilms (cfu cm−2 min−1) with time was shown for each biofilm, regardless of the prevailing shear. As the shear rate was increased beyond 0.123 ms−1, populations tended toward a pseudo steady-state. Sudden changes in shear force, however, caused dramatic changes in the productivity of steady-state populations. The CCR provides an effective means of testing disinfectant activity, particularly for clean-in-place situations, and allows for an examination of the residual effects of a cleansing programme on a treated surface for three different chemical classes of disinfectant. Utilisation of the CCR would, therefore, provide enhanced ability to determine the efficacy and efficiency of chemical products for use in sanitation protocols.",
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A new technique for the performance evaluation of clean-in-place disinfection of biofilms. / Willcock, L.; Gilbert, P.; Holah, J.; Wirtanen, Gun; Allison, D. G.

In: Journal of industrial microbiology and biotechnology, Vol. 25, No. 5, 2000, p. 235 - 241.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - A new technique for the performance evaluation of clean-in-place disinfection of biofilms

AU - Willcock, L.

AU - Gilbert, P.

AU - Holah, J.

AU - Wirtanen, Gun

AU - Allison, D. G.

PY - 2000

Y1 - 2000

N2 - A concentric cylinder reactor (CCR) is described that enables the steady-state kinetics of microbial biofilms to be evaluated under conditions of constant nutrient flow and variable shear-stress. The reactor has been used to evaluate the influence of fluid dynamic shear on the extent and mode of detachment of bacteria from biofilms. Using a food factory isolate of Pseudomonas aeruginosa, a general increase in the overall growth rate and detachment of the biofilms (cfu cm−2 min−1) with time was shown for each biofilm, regardless of the prevailing shear. As the shear rate was increased beyond 0.123 ms−1, populations tended toward a pseudo steady-state. Sudden changes in shear force, however, caused dramatic changes in the productivity of steady-state populations. The CCR provides an effective means of testing disinfectant activity, particularly for clean-in-place situations, and allows for an examination of the residual effects of a cleansing programme on a treated surface for three different chemical classes of disinfectant. Utilisation of the CCR would, therefore, provide enhanced ability to determine the efficacy and efficiency of chemical products for use in sanitation protocols.

AB - A concentric cylinder reactor (CCR) is described that enables the steady-state kinetics of microbial biofilms to be evaluated under conditions of constant nutrient flow and variable shear-stress. The reactor has been used to evaluate the influence of fluid dynamic shear on the extent and mode of detachment of bacteria from biofilms. Using a food factory isolate of Pseudomonas aeruginosa, a general increase in the overall growth rate and detachment of the biofilms (cfu cm−2 min−1) with time was shown for each biofilm, regardless of the prevailing shear. As the shear rate was increased beyond 0.123 ms−1, populations tended toward a pseudo steady-state. Sudden changes in shear force, however, caused dramatic changes in the productivity of steady-state populations. The CCR provides an effective means of testing disinfectant activity, particularly for clean-in-place situations, and allows for an examination of the residual effects of a cleansing programme on a treated surface for three different chemical classes of disinfectant. Utilisation of the CCR would, therefore, provide enhanced ability to determine the efficacy and efficiency of chemical products for use in sanitation protocols.

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KW - biofilm

KW - disinfection

KW - biocides

KW - fluid dynamic shear

U2 - 10.1038/sj.jim.7000063

DO - 10.1038/sj.jim.7000063

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JO - Journal of industrial microbiology and biotechnology

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