A note on the use of dinitrosalicylic acid for determining the products of enzymatic reactions

Michael Bailey

Research output: Contribution to journalArticleScientificpeer-review

88 Citations (Scopus)

Abstract

Factors contributing to non-linearity of enzyme assays incorporating detection of reaction products using dinitrosalicylic acid (DNS) are discussed.
The common practice of diluting reaction products before quantification of reducing compounds is shown to be one cause of non-linearity. Insufficiency of substrate is also an important contributory factor in most modern versions of the method, although the original procedure was correctly designed to ensure a linear reaction.
The inherent inaccuracy involved in expression of the results of non-linear reactions in units implying linearity (katals or International Units) is emphasized.
Original languageEnglish
Pages (from-to)494-496
JournalApplied Microbiology and Biotechnology
Volume29
Issue number5
DOIs
Publication statusPublished - 1988
MoE publication typeA1 Journal article-refereed

Fingerprint

Acids
Enzyme Assays

Cite this

@article{3138e3a06c2b40228c900aef574a0636,
title = "A note on the use of dinitrosalicylic acid for determining the products of enzymatic reactions",
abstract = "Factors contributing to non-linearity of enzyme assays incorporating detection of reaction products using dinitrosalicylic acid (DNS) are discussed. The common practice of diluting reaction products before quantification of reducing compounds is shown to be one cause of non-linearity. Insufficiency of substrate is also an important contributory factor in most modern versions of the method, although the original procedure was correctly designed to ensure a linear reaction. The inherent inaccuracy involved in expression of the results of non-linear reactions in units implying linearity (katals or International Units) is emphasized.",
author = "Michael Bailey",
year = "1988",
doi = "10.1007/BF00269074",
language = "English",
volume = "29",
pages = "494--496",
journal = "Applied Microbiology and Biotechnology",
issn = "0175-7598",
publisher = "Springer",
number = "5",

}

A note on the use of dinitrosalicylic acid for determining the products of enzymatic reactions. / Bailey, Michael.

In: Applied Microbiology and Biotechnology, Vol. 29, No. 5, 1988, p. 494-496.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - A note on the use of dinitrosalicylic acid for determining the products of enzymatic reactions

AU - Bailey, Michael

PY - 1988

Y1 - 1988

N2 - Factors contributing to non-linearity of enzyme assays incorporating detection of reaction products using dinitrosalicylic acid (DNS) are discussed. The common practice of diluting reaction products before quantification of reducing compounds is shown to be one cause of non-linearity. Insufficiency of substrate is also an important contributory factor in most modern versions of the method, although the original procedure was correctly designed to ensure a linear reaction. The inherent inaccuracy involved in expression of the results of non-linear reactions in units implying linearity (katals or International Units) is emphasized.

AB - Factors contributing to non-linearity of enzyme assays incorporating detection of reaction products using dinitrosalicylic acid (DNS) are discussed. The common practice of diluting reaction products before quantification of reducing compounds is shown to be one cause of non-linearity. Insufficiency of substrate is also an important contributory factor in most modern versions of the method, although the original procedure was correctly designed to ensure a linear reaction. The inherent inaccuracy involved in expression of the results of non-linear reactions in units implying linearity (katals or International Units) is emphasized.

U2 - 10.1007/BF00269074

DO - 10.1007/BF00269074

M3 - Article

VL - 29

SP - 494

EP - 496

JO - Applied Microbiology and Biotechnology

JF - Applied Microbiology and Biotechnology

SN - 0175-7598

IS - 5

ER -