A novel class of fungal lipoxygenases

R. Heshof, Sirpa Jylhä, T. Haarmann, A.L.W. Jørgensen, T.K. Dalsgaard, L.H. de Graaff (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

15 Citations (Scopus)

Abstract

Lipoxygenases (LOXs) are well-studied enzymes in plants and mammals. However, fungal LOXs are less studied. In this study, we have compared fungal LOX protein sequences to all known characterized LOXs. For this, a script was written using Shell commands to extract sequences from the NCBI database and to align the sequences obtained using Multiple Sequence Comparison by Log-Expectation. We constructed a phylogenetic tree with the use of Quicktree to visualize the relation of fungal LOXs towards other LOXs. These sequences were analyzed with respect to the signal sequence, C-terminal amino acid, the stereochemistry of the formed oxylipin, and the metal ion cofactor usage. This study shows fungal LOXs are divided into two groups, the Ile- and the Val-groups. The Ile-group has a conserved WRYAK sequence that appears to be characteristic for fungal LOXs and has as a C-terminal amino acid Ile. The Val-group has a highly conserved WL-L/F-AK sequence that is also found in LOXs of plant and animal origin. We found that fungal LOXs with this conserved sequence have a Val at the C-terminus in contrast to other LOXs of fungal origin. Also, these LOXs have signal sequences implying these LOXs will be expressed extracellularly. Our results show that in this group, in addition to the Gaeumannomyces graminis and the Magnaporthe salvinii LOXs, the Aspergillus fumigatus LOX uses manganese as a cofactor.
Original languageEnglish
Pages (from-to)1261-1270
JournalApplied Microbiology and Biotechnology
Volume98
Issue number3
DOIs
Publication statusPublished - 2014
MoE publication typeA1 Journal article-refereed

Fingerprint

Lipoxygenases
Conserved Sequence
Protein Sorting Signals
Oxylipins
Magnaporthe
Amino Acids
Aspergillus fumigatus
Fungal Proteins

Keywords

  • aspergillus fumigatus
  • cofactor
  • gaeumannomyces graminis
  • lipoxygenase
  • manganese
  • phylogenetic tree

Cite this

Heshof, R., Jylhä, S., Haarmann, T., Jørgensen, A. L. W., Dalsgaard, T. K., & de Graaff, L. H. (2014). A novel class of fungal lipoxygenases. Applied Microbiology and Biotechnology, 98(3), 1261-1270. https://doi.org/10.1007/s00253-013-5392-x
Heshof, R. ; Jylhä, Sirpa ; Haarmann, T. ; Jørgensen, A.L.W. ; Dalsgaard, T.K. ; de Graaff, L.H. / A novel class of fungal lipoxygenases. In: Applied Microbiology and Biotechnology. 2014 ; Vol. 98, No. 3. pp. 1261-1270.
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Heshof, R, Jylhä, S, Haarmann, T, Jørgensen, ALW, Dalsgaard, TK & de Graaff, LH 2014, 'A novel class of fungal lipoxygenases', Applied Microbiology and Biotechnology, vol. 98, no. 3, pp. 1261-1270. https://doi.org/10.1007/s00253-013-5392-x

A novel class of fungal lipoxygenases. / Heshof, R.; Jylhä, Sirpa; Haarmann, T.; Jørgensen, A.L.W.; Dalsgaard, T.K.; de Graaff, L.H. (Corresponding Author).

In: Applied Microbiology and Biotechnology, Vol. 98, No. 3, 2014, p. 1261-1270.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - A novel class of fungal lipoxygenases

AU - Heshof, R.

AU - Jylhä, Sirpa

AU - Haarmann, T.

AU - Jørgensen, A.L.W.

AU - Dalsgaard, T.K.

AU - de Graaff, L.H.

PY - 2014

Y1 - 2014

N2 - Lipoxygenases (LOXs) are well-studied enzymes in plants and mammals. However, fungal LOXs are less studied. In this study, we have compared fungal LOX protein sequences to all known characterized LOXs. For this, a script was written using Shell commands to extract sequences from the NCBI database and to align the sequences obtained using Multiple Sequence Comparison by Log-Expectation. We constructed a phylogenetic tree with the use of Quicktree to visualize the relation of fungal LOXs towards other LOXs. These sequences were analyzed with respect to the signal sequence, C-terminal amino acid, the stereochemistry of the formed oxylipin, and the metal ion cofactor usage. This study shows fungal LOXs are divided into two groups, the Ile- and the Val-groups. The Ile-group has a conserved WRYAK sequence that appears to be characteristic for fungal LOXs and has as a C-terminal amino acid Ile. The Val-group has a highly conserved WL-L/F-AK sequence that is also found in LOXs of plant and animal origin. We found that fungal LOXs with this conserved sequence have a Val at the C-terminus in contrast to other LOXs of fungal origin. Also, these LOXs have signal sequences implying these LOXs will be expressed extracellularly. Our results show that in this group, in addition to the Gaeumannomyces graminis and the Magnaporthe salvinii LOXs, the Aspergillus fumigatus LOX uses manganese as a cofactor.

AB - Lipoxygenases (LOXs) are well-studied enzymes in plants and mammals. However, fungal LOXs are less studied. In this study, we have compared fungal LOX protein sequences to all known characterized LOXs. For this, a script was written using Shell commands to extract sequences from the NCBI database and to align the sequences obtained using Multiple Sequence Comparison by Log-Expectation. We constructed a phylogenetic tree with the use of Quicktree to visualize the relation of fungal LOXs towards other LOXs. These sequences were analyzed with respect to the signal sequence, C-terminal amino acid, the stereochemistry of the formed oxylipin, and the metal ion cofactor usage. This study shows fungal LOXs are divided into two groups, the Ile- and the Val-groups. The Ile-group has a conserved WRYAK sequence that appears to be characteristic for fungal LOXs and has as a C-terminal amino acid Ile. The Val-group has a highly conserved WL-L/F-AK sequence that is also found in LOXs of plant and animal origin. We found that fungal LOXs with this conserved sequence have a Val at the C-terminus in contrast to other LOXs of fungal origin. Also, these LOXs have signal sequences implying these LOXs will be expressed extracellularly. Our results show that in this group, in addition to the Gaeumannomyces graminis and the Magnaporthe salvinii LOXs, the Aspergillus fumigatus LOX uses manganese as a cofactor.

KW - aspergillus fumigatus

KW - cofactor

KW - gaeumannomyces graminis

KW - lipoxygenase

KW - manganese

KW - phylogenetic tree

U2 - 10.1007/s00253-013-5392-x

DO - 10.1007/s00253-013-5392-x

M3 - Article

VL - 98

SP - 1261

EP - 1270

JO - Applied Microbiology and Biotechnology

JF - Applied Microbiology and Biotechnology

SN - 0175-7598

IS - 3

ER -

Heshof R, Jylhä S, Haarmann T, Jørgensen ALW, Dalsgaard TK, de Graaff LH. A novel class of fungal lipoxygenases. Applied Microbiology and Biotechnology. 2014;98(3):1261-1270. https://doi.org/10.1007/s00253-013-5392-x