A novel combination of prosthetic groups in a fungal laccase, PQQ and two copper atoms

Eija Karhunen, Marja-Leena Niku-Paavola (Corresponding Author), Liisa Viikari, Tuomas Haltia, Rob van der Meer, Johannis Duine

Research output: Contribution to journalArticleScientificpeer-review

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Abstract

Extracellular laccase (benzenediol:oxygen oxidoreductase EC 1.10.3.2) from the lignin‐degrading fungus, Phlebia radiata, was shown to contain a novel combination of electron carriers as its prosthetic groups. In addition to two copper atoms per enzyme molecule, one molecule of PQQ was included as a cofactor. The EPR spectrum exhibits features of type 1 and type 2 copper atoms. In the enzymatic reaction 4 molecules of lignin model compound, coniferyl alcohol, are oxidized per molecule of oxygen reduced to water. During the reaction coniferyl alcohol is transformed to dilignols.

Original languageEnglish
Pages (from-to)6-8
JournalFEBS Letters
Volume267
Issue number1
DOIs
Publication statusPublished - 1990
MoE publication typeA1 Journal article-refereed

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Laccase
Prosthetics
Copper
Oxygen
Atoms
Molecules
Lignin
Oxidoreductases
Fungi
Electrons
Water
Enzymes
Paramagnetic resonance
coniferyl alcohol

Cite this

Karhunen, E., Niku-Paavola, M-L., Viikari, L., Haltia, T., Meer, R. V. D., & Duine, J. (1990). A novel combination of prosthetic groups in a fungal laccase, PQQ and two copper atoms. FEBS Letters, 267(1), 6-8. https://doi.org/10.1016/0014-5793(90)80273-L
Karhunen, Eija ; Niku-Paavola, Marja-Leena ; Viikari, Liisa ; Haltia, Tuomas ; Meer, Rob van der ; Duine, Johannis. / A novel combination of prosthetic groups in a fungal laccase, PQQ and two copper atoms. In: FEBS Letters. 1990 ; Vol. 267, No. 1. pp. 6-8.
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abstract = "Extracellular laccase (benzenediol:oxygen oxidoreductase EC 1.10.3.2) from the lignin‐degrading fungus, Phlebia radiata, was shown to contain a novel combination of electron carriers as its prosthetic groups. In addition to two copper atoms per enzyme molecule, one molecule of PQQ was included as a cofactor. The EPR spectrum exhibits features of type 1 and type 2 copper atoms. In the enzymatic reaction 4 molecules of lignin model compound, coniferyl alcohol, are oxidized per molecule of oxygen reduced to water. During the reaction coniferyl alcohol is transformed to dilignols.",
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Karhunen, E, Niku-Paavola, M-L, Viikari, L, Haltia, T, Meer, RVD & Duine, J 1990, 'A novel combination of prosthetic groups in a fungal laccase, PQQ and two copper atoms', FEBS Letters, vol. 267, no. 1, pp. 6-8. https://doi.org/10.1016/0014-5793(90)80273-L

A novel combination of prosthetic groups in a fungal laccase, PQQ and two copper atoms. / Karhunen, Eija; Niku-Paavola, Marja-Leena (Corresponding Author); Viikari, Liisa; Haltia, Tuomas; Meer, Rob van der; Duine, Johannis.

In: FEBS Letters, Vol. 267, No. 1, 1990, p. 6-8.

Research output: Contribution to journalArticleScientificpeer-review

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T1 - A novel combination of prosthetic groups in a fungal laccase, PQQ and two copper atoms

AU - Karhunen, Eija

AU - Niku-Paavola, Marja-Leena

AU - Viikari, Liisa

AU - Haltia, Tuomas

AU - Meer, Rob van der

AU - Duine, Johannis

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AB - Extracellular laccase (benzenediol:oxygen oxidoreductase EC 1.10.3.2) from the lignin‐degrading fungus, Phlebia radiata, was shown to contain a novel combination of electron carriers as its prosthetic groups. In addition to two copper atoms per enzyme molecule, one molecule of PQQ was included as a cofactor. The EPR spectrum exhibits features of type 1 and type 2 copper atoms. In the enzymatic reaction 4 molecules of lignin model compound, coniferyl alcohol, are oxidized per molecule of oxygen reduced to water. During the reaction coniferyl alcohol is transformed to dilignols.

U2 - 10.1016/0014-5793(90)80273-L

DO - 10.1016/0014-5793(90)80273-L

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VL - 267

SP - 6

EP - 8

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

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