A novel combination of prosthetic groups in a fungal laccase, PQQ and two copper atoms

Eija Karhunen; Marja-Leena Niku-Paavola; Liisa Viikari; Tuomas Haltia; Rob van der Meer; Johannis Duine

doi:10.1016/0014-5793(90)80273-L

A novel combination of prosthetic groups in a fungal laccase, PQQ and two copper atoms

Eija Karhunen
, Marja-Leena Niku-Paavola^*
, Liisa Viikari
, Tuomas Haltia
, Rob van der Meer
, Johannis Duine

^*Corresponding author for this work

VTT Technical Research Centre of Finland

University of Helsinki
Delft University of Technology

Research output: Contribution to journal › Article › Scientific › peer-review

47 Citations (Scopus)

Abstract

Extracellular laccase (benzenediol:oxygen oxidoreductase EC 1.10.3.2) from the lignin‐degrading fungus, Phlebia radiata, was shown to contain a novel combination of electron carriers as its prosthetic groups. In addition to two copper atoms per enzyme molecule, one molecule of PQQ was included as a cofactor. The EPR spectrum exhibits features of type 1 and type 2 copper atoms. In the enzymatic reaction 4 molecules of lignin model compound, coniferyl alcohol, are oxidized per molecule of oxygen reduced to water. During the reaction coniferyl alcohol is transformed to dilignols.

Original language	English
Pages (from-to)	6-8
Journal	FEBS Letters
Volume	267
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(90)80273-L
Publication status	Published - 1990
MoE publication type	A1 Journal article-refereed

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title = "A novel combination of prosthetic groups in a fungal laccase, PQQ and two copper atoms",

abstract = "Extracellular laccase (benzenediol:oxygen oxidoreductase EC 1.10.3.2) from the lignin‐degrading fungus, Phlebia radiata, was shown to contain a novel combination of electron carriers as its prosthetic groups. In addition to two copper atoms per enzyme molecule, one molecule of PQQ was included as a cofactor. The EPR spectrum exhibits features of type 1 and type 2 copper atoms. In the enzymatic reaction 4 molecules of lignin model compound, coniferyl alcohol, are oxidized per molecule of oxygen reduced to water. During the reaction coniferyl alcohol is transformed to dilignols. ",

author = "Eija Karhunen and Marja-Leena Niku-Paavola and Liisa Viikari and Tuomas Haltia and Meer, \{Rob van der\} and Johannis Duine",

year = "1990",

doi = "10.1016/0014-5793(90)80273-L",

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T1 - A novel combination of prosthetic groups in a fungal laccase, PQQ and two copper atoms

AU - Karhunen, Eija

AU - Niku-Paavola, Marja-Leena

AU - Viikari, Liisa

AU - Haltia, Tuomas

AU - Meer, Rob van der

AU - Duine, Johannis

PY - 1990

Y1 - 1990

N2 - Extracellular laccase (benzenediol:oxygen oxidoreductase EC 1.10.3.2) from the lignin‐degrading fungus, Phlebia radiata, was shown to contain a novel combination of electron carriers as its prosthetic groups. In addition to two copper atoms per enzyme molecule, one molecule of PQQ was included as a cofactor. The EPR spectrum exhibits features of type 1 and type 2 copper atoms. In the enzymatic reaction 4 molecules of lignin model compound, coniferyl alcohol, are oxidized per molecule of oxygen reduced to water. During the reaction coniferyl alcohol is transformed to dilignols.

AB - Extracellular laccase (benzenediol:oxygen oxidoreductase EC 1.10.3.2) from the lignin‐degrading fungus, Phlebia radiata, was shown to contain a novel combination of electron carriers as its prosthetic groups. In addition to two copper atoms per enzyme molecule, one molecule of PQQ was included as a cofactor. The EPR spectrum exhibits features of type 1 and type 2 copper atoms. In the enzymatic reaction 4 molecules of lignin model compound, coniferyl alcohol, are oxidized per molecule of oxygen reduced to water. During the reaction coniferyl alcohol is transformed to dilignols.

U2 - 10.1016/0014-5793(90)80273-L

DO - 10.1016/0014-5793(90)80273-L

M3 - Article

SN - 0014-5793

VL - 267

SP - 6

EP - 8

JO - FEBS Letters

JF - FEBS Letters

IS - 1

ER -

A novel combination of prosthetic groups in a fungal laccase, PQQ and two copper atoms

Abstract

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