A novel laccase from the ascomycete Melanocarpus albomyces

Kristiina Kruus, Laura-Leena Kiiskinen, Markku Saloheimo, Nina Hakulinen, Juha Rouvinen, Arja Paananen, Markus Linder, Liisa Viikari

Research output: Chapter in Book/Report/Conference proceedingConference article in proceedingsScientificpeer-review

Abstract

A novel laccase from the ascomycete Melanocarpus albomyces was isolated, purified and characterized. The ultraviolet-visible absorption and electron paramagnetic resonance spectra indicated that the three types of coppers were present. Redox potential of the T1 copper of M. albomyces laccase was determined to be 0.46±0.01 V. The enzyme had very interesting pH and temperature behaviour. It had a pH optimum measured with guaiacol at neutral and slightly alkalic pH and substantial activity still at pH 8. The laccase showed very good thermostability, retaining full activity for two hours at 60°C. The gene encoding the M. albomyces laccase was isolated and sequenced. The length of the open reading frame of the M. albomyces laccase was 623 amino acid residues. The copper binding residues were well conserved and the amino acid sequence had high homology to other ascomycete laccases. Interestingly the secreted laccase was processed both from the amino and carboxy terminus. The laccase was also crystallized with all four coppers present.
Original languageEnglish
Title of host publicationApplications of Enzymes to Lignocellulosics
EditorsShawn D. Mansfield, John N. Saddler
Place of PublicationWashington, DC
PublisherAmerican Chemical Society ACS
Chapter19
Pages315-331
ISBN (Electronic)978-0-8412-1960-1
ISBN (Print)978-0-8412-3831-2
DOIs
Publication statusPublished - 2003
MoE publication typeA4 Article in a conference publication

Publication series

SeriesACS Symposium Series
Volume855

Fingerprint Dive into the research topics of 'A novel laccase from the ascomycete Melanocarpus albomyces'. Together they form a unique fingerprint.

  • Cite this

    Kruus, K., Kiiskinen, L-L., Saloheimo, M., Hakulinen, N., Rouvinen, J., Paananen, A., Linder, M., & Viikari, L. (2003). A novel laccase from the ascomycete Melanocarpus albomyces. In S. D. Mansfield, & J. N. Saddler (Eds.), Applications of Enzymes to Lignocellulosics (pp. 315-331). American Chemical Society ACS. ACS Symposium Series, Vol.. 855 https://doi.org/10.1021/bk-2003-0855.ch019