Abstract
A highly surface-sensitive fluorescent measurement platform for chemical
and biochemical analysis is presented. The core component of the
platform is a polystyrene parabolic lens that is utilised in
simultaneous total internal reflection excitation and super critical
angle fluorescence detection. Both the theoretical calculations and the
experimental results of the immunological model assays show that the
system is extremely sensitive to the surface bound fluorescence. The
binding of analytes at concentrations down to 5 pmol/l has been detected
in real time while the unbound fluorescent labels in the sample liquid
did not show any response until at three decades higher concentrations.
| Original language | English |
|---|---|
| Pages (from-to) | 358 - 362 |
| Number of pages | 5 |
| Journal | Chemical Physics Letters |
| Volume | 473 |
| Issue number | 4 - 6 |
| DOIs | |
| Publication status | Published - 2009 |
| MoE publication type | A1 Journal article-refereed |
Keywords
- fluorescent measurements
- fluorescence
- fluorescence microscopy
- fluorescence techniques