A novel platform for highly surface-sensitive fluorescent measurements applying simultaneous total internal reflection excitation and super critical angle detection

Hannu Välimäki (Corresponding Author), Kirsi Tappura

    Research output: Contribution to journalArticleScientificpeer-review

    10 Citations (Scopus)

    Abstract

    A highly surface-sensitive fluorescent measurement platform for chemical and biochemical analysis is presented. The core component of the platform is a polystyrene parabolic lens that is utilised in simultaneous total internal reflection excitation and super critical angle fluorescence detection. Both the theoretical calculations and the experimental results of the immunological model assays show that the system is extremely sensitive to the surface bound fluorescence. The binding of analytes at concentrations down to 5 pmol/l has been detected in real time while the unbound fluorescent labels in the sample liquid did not show any response until at three decades higher concentrations.
    Original languageEnglish
    Pages (from-to)358 - 362
    Number of pages5
    JournalChemical Physics Letters
    Volume473
    Issue number4 - 6
    DOIs
    Publication statusPublished - 2009
    MoE publication typeA1 Journal article-refereed

    Fingerprint

    platforms
    Fluorescence
    fluorescence
    Polystyrenes
    chemical analysis
    excitation
    Labels
    Lenses
    Assays
    polystyrene
    lenses
    Liquids
    liquids

    Keywords

    • fluorescent measurements
    • fluorescence
    • fluorescence microscopy
    • fluorescence techniques

    Cite this

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    title = "A novel platform for highly surface-sensitive fluorescent measurements applying simultaneous total internal reflection excitation and super critical angle detection",
    abstract = "A highly surface-sensitive fluorescent measurement platform for chemical and biochemical analysis is presented. The core component of the platform is a polystyrene parabolic lens that is utilised in simultaneous total internal reflection excitation and super critical angle fluorescence detection. Both the theoretical calculations and the experimental results of the immunological model assays show that the system is extremely sensitive to the surface bound fluorescence. The binding of analytes at concentrations down to 5 pmol/l has been detected in real time while the unbound fluorescent labels in the sample liquid did not show any response until at three decades higher concentrations.",
    keywords = "fluorescent measurements, fluorescence, fluorescence microscopy, fluorescence techniques",
    author = "Hannu V{\"a}lim{\"a}ki and Kirsi Tappura",
    year = "2009",
    doi = "10.1016/j.cplett.2009.04.010",
    language = "English",
    volume = "473",
    pages = "358 -- 362",
    journal = "Chemical Physics Letters",
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    A novel platform for highly surface-sensitive fluorescent measurements applying simultaneous total internal reflection excitation and super critical angle detection. / Välimäki, Hannu (Corresponding Author); Tappura, Kirsi.

    In: Chemical Physics Letters, Vol. 473, No. 4 - 6, 2009, p. 358 - 362.

    Research output: Contribution to journalArticleScientificpeer-review

    TY - JOUR

    T1 - A novel platform for highly surface-sensitive fluorescent measurements applying simultaneous total internal reflection excitation and super critical angle detection

    AU - Välimäki, Hannu

    AU - Tappura, Kirsi

    PY - 2009

    Y1 - 2009

    N2 - A highly surface-sensitive fluorescent measurement platform for chemical and biochemical analysis is presented. The core component of the platform is a polystyrene parabolic lens that is utilised in simultaneous total internal reflection excitation and super critical angle fluorescence detection. Both the theoretical calculations and the experimental results of the immunological model assays show that the system is extremely sensitive to the surface bound fluorescence. The binding of analytes at concentrations down to 5 pmol/l has been detected in real time while the unbound fluorescent labels in the sample liquid did not show any response until at three decades higher concentrations.

    AB - A highly surface-sensitive fluorescent measurement platform for chemical and biochemical analysis is presented. The core component of the platform is a polystyrene parabolic lens that is utilised in simultaneous total internal reflection excitation and super critical angle fluorescence detection. Both the theoretical calculations and the experimental results of the immunological model assays show that the system is extremely sensitive to the surface bound fluorescence. The binding of analytes at concentrations down to 5 pmol/l has been detected in real time while the unbound fluorescent labels in the sample liquid did not show any response until at three decades higher concentrations.

    KW - fluorescent measurements

    KW - fluorescence

    KW - fluorescence microscopy

    KW - fluorescence techniques

    U2 - 10.1016/j.cplett.2009.04.010

    DO - 10.1016/j.cplett.2009.04.010

    M3 - Article

    VL - 473

    SP - 358

    EP - 362

    JO - Chemical Physics Letters

    JF - Chemical Physics Letters

    SN - 0009-2614

    IS - 4 - 6

    ER -