A novel platform for highly surface-sensitive fluorescent measurements applying simultaneous total internal reflection excitation and super critical angle detection

Hannu Välimäki (Corresponding Author), Kirsi Tappura

Research output: Contribution to journalArticleScientificpeer-review

10 Citations (Scopus)

Abstract

A highly surface-sensitive fluorescent measurement platform for chemical and biochemical analysis is presented. The core component of the platform is a polystyrene parabolic lens that is utilised in simultaneous total internal reflection excitation and super critical angle fluorescence detection. Both the theoretical calculations and the experimental results of the immunological model assays show that the system is extremely sensitive to the surface bound fluorescence. The binding of analytes at concentrations down to 5 pmol/l has been detected in real time while the unbound fluorescent labels in the sample liquid did not show any response until at three decades higher concentrations.
Original languageEnglish
Pages (from-to)358 - 362
Number of pages5
JournalChemical Physics Letters
Volume473
Issue number4 - 6
DOIs
Publication statusPublished - 2009
MoE publication typeA1 Journal article-refereed

Fingerprint

platforms
Fluorescence
fluorescence
Polystyrenes
chemical analysis
excitation
Labels
Lenses
Assays
polystyrene
lenses
Liquids
liquids

Keywords

  • fluorescent measurements
  • fluorescence
  • fluorescence microscopy
  • fluorescence techniques

Cite this

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abstract = "A highly surface-sensitive fluorescent measurement platform for chemical and biochemical analysis is presented. The core component of the platform is a polystyrene parabolic lens that is utilised in simultaneous total internal reflection excitation and super critical angle fluorescence detection. Both the theoretical calculations and the experimental results of the immunological model assays show that the system is extremely sensitive to the surface bound fluorescence. The binding of analytes at concentrations down to 5 pmol/l has been detected in real time while the unbound fluorescent labels in the sample liquid did not show any response until at three decades higher concentrations.",
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journal = "Chemical Physics Letters",
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A novel platform for highly surface-sensitive fluorescent measurements applying simultaneous total internal reflection excitation and super critical angle detection. / Välimäki, Hannu (Corresponding Author); Tappura, Kirsi.

In: Chemical Physics Letters, Vol. 473, No. 4 - 6, 2009, p. 358 - 362.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - A novel platform for highly surface-sensitive fluorescent measurements applying simultaneous total internal reflection excitation and super critical angle detection

AU - Välimäki, Hannu

AU - Tappura, Kirsi

PY - 2009

Y1 - 2009

N2 - A highly surface-sensitive fluorescent measurement platform for chemical and biochemical analysis is presented. The core component of the platform is a polystyrene parabolic lens that is utilised in simultaneous total internal reflection excitation and super critical angle fluorescence detection. Both the theoretical calculations and the experimental results of the immunological model assays show that the system is extremely sensitive to the surface bound fluorescence. The binding of analytes at concentrations down to 5 pmol/l has been detected in real time while the unbound fluorescent labels in the sample liquid did not show any response until at three decades higher concentrations.

AB - A highly surface-sensitive fluorescent measurement platform for chemical and biochemical analysis is presented. The core component of the platform is a polystyrene parabolic lens that is utilised in simultaneous total internal reflection excitation and super critical angle fluorescence detection. Both the theoretical calculations and the experimental results of the immunological model assays show that the system is extremely sensitive to the surface bound fluorescence. The binding of analytes at concentrations down to 5 pmol/l has been detected in real time while the unbound fluorescent labels in the sample liquid did not show any response until at three decades higher concentrations.

KW - fluorescent measurements

KW - fluorescence

KW - fluorescence microscopy

KW - fluorescence techniques

U2 - 10.1016/j.cplett.2009.04.010

DO - 10.1016/j.cplett.2009.04.010

M3 - Article

VL - 473

SP - 358

EP - 362

JO - Chemical Physics Letters

JF - Chemical Physics Letters

SN - 0009-2614

IS - 4 - 6

ER -