Abstract
Morphine was used as a model analyte to examine the
possibility of using cellulose, physically modified by
papermaking and converting techniques, as a capillary
matrix in a lateral flow type of diagnostic assay. This
research was directed toward low-cost, disposable, and
portable paperbased diagnostics, with the aim of
addressing the analytical performance of paper as a
substrate in the analysis for drugs of abuse. Antibody
Fab fragments were used as sensing molecules, and gold
nanoparticle detection was employed. Inkjet printing was
used to pattern sensing biomolecules as detection zones
on paper. To validate the usefulness of paper as a
diagnostic platform, the principle of a direct sandwich
assay, based on immunocomplex formation between morphine
and the anti-morphine Fab fragment and detection of the
formed immunocomplex by another Fab fragment, was
implemented. Results were compared with that achieved by
using nitrocellulose as a reference material. Possible
interfering from the sample matrix on assay quality was
investigated with spiked oral fluid samples. Under
optimized conditions, a visually assessed limit of
detection for the sandwich assay was 1 ng/mL, indicating
that the paper-based test devices developed in this work
can perform screening for drugs of abuse and can fulfill
the requirement for a sensitive assay in diagnostically
relevant ranges
Original language | English |
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Pages (from-to) | 5955-5965 |
Journal | Analytical and Bioanalytical Chemistry |
Volume | 406 |
Issue number | 24 |
DOIs | |
Publication status | Published - 2014 |
MoE publication type | A1 Journal article-refereed |
Keywords
- paper-based
- drugs of abuse
- recombinant antibody
- immunocomplex assay
- point-of-care diagnostics
- low resources
- capillary actions
- cellulose