A paper-based lateral flow assay for morphine

Tuija Teerinen (Corresponding Author), Timo Lappalainen, Tomi Erho

Research output: Contribution to journalArticleScientificpeer-review

20 Citations (Scopus)

Abstract

Morphine was used as a model analyte to examine the possibility of using cellulose, physically modified by papermaking and converting techniques, as a capillary matrix in a lateral flow type of diagnostic assay. This research was directed toward low-cost, disposable, and portable paperbased diagnostics, with the aim of addressing the analytical performance of paper as a substrate in the analysis for drugs of abuse. Antibody Fab fragments were used as sensing molecules, and gold nanoparticle detection was employed. Inkjet printing was used to pattern sensing biomolecules as detection zones on paper. To validate the usefulness of paper as a diagnostic platform, the principle of a direct sandwich assay, based on immunocomplex formation between morphine and the anti-morphine Fab fragment and detection of the formed immunocomplex by another Fab fragment, was implemented. Results were compared with that achieved by using nitrocellulose as a reference material. Possible interfering from the sample matrix on assay quality was investigated with spiked oral fluid samples. Under optimized conditions, a visually assessed limit of detection for the sandwich assay was 1 ng/mL, indicating that the paper-based test devices developed in this work can perform screening for drugs of abuse and can fulfill the requirement for a sensitive assay in diagnostically relevant ranges
Original languageEnglish
Pages (from-to)5955-5965
JournalAnalytical and Bioanalytical Chemistry
Volume406
Issue number24
DOIs
Publication statusPublished - 2014
MoE publication typeA1 Journal article-refereed

Fingerprint

Immunoglobulin Fab Fragments
Morphine
Assays
Street Drugs
Immunoglobulin Fragments
Printing
Collodion
Cellulose
Gold
Nanoparticles
Limit of Detection
Papermaking
Costs and Cost Analysis
Biomolecules
Equipment and Supplies
Screening
Research
Molecules
Fluids
Antibodies

Keywords

  • paper-based
  • drugs of abuse
  • recombinant antibody
  • immunocomplex assay
  • point-of-care diagnostics
  • low resources
  • capillary actions
  • cellulose

Cite this

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title = "A paper-based lateral flow assay for morphine",
abstract = "Morphine was used as a model analyte to examine the possibility of using cellulose, physically modified by papermaking and converting techniques, as a capillary matrix in a lateral flow type of diagnostic assay. This research was directed toward low-cost, disposable, and portable paperbased diagnostics, with the aim of addressing the analytical performance of paper as a substrate in the analysis for drugs of abuse. Antibody Fab fragments were used as sensing molecules, and gold nanoparticle detection was employed. Inkjet printing was used to pattern sensing biomolecules as detection zones on paper. To validate the usefulness of paper as a diagnostic platform, the principle of a direct sandwich assay, based on immunocomplex formation between morphine and the anti-morphine Fab fragment and detection of the formed immunocomplex by another Fab fragment, was implemented. Results were compared with that achieved by using nitrocellulose as a reference material. Possible interfering from the sample matrix on assay quality was investigated with spiked oral fluid samples. Under optimized conditions, a visually assessed limit of detection for the sandwich assay was 1 ng/mL, indicating that the paper-based test devices developed in this work can perform screening for drugs of abuse and can fulfill the requirement for a sensitive assay in diagnostically relevant ranges",
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A paper-based lateral flow assay for morphine. / Teerinen, Tuija (Corresponding Author); Lappalainen, Timo; Erho, Tomi.

In: Analytical and Bioanalytical Chemistry, Vol. 406, No. 24, 2014, p. 5955-5965.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - A paper-based lateral flow assay for morphine

AU - Teerinen, Tuija

AU - Lappalainen, Timo

AU - Erho, Tomi

PY - 2014

Y1 - 2014

N2 - Morphine was used as a model analyte to examine the possibility of using cellulose, physically modified by papermaking and converting techniques, as a capillary matrix in a lateral flow type of diagnostic assay. This research was directed toward low-cost, disposable, and portable paperbased diagnostics, with the aim of addressing the analytical performance of paper as a substrate in the analysis for drugs of abuse. Antibody Fab fragments were used as sensing molecules, and gold nanoparticle detection was employed. Inkjet printing was used to pattern sensing biomolecules as detection zones on paper. To validate the usefulness of paper as a diagnostic platform, the principle of a direct sandwich assay, based on immunocomplex formation between morphine and the anti-morphine Fab fragment and detection of the formed immunocomplex by another Fab fragment, was implemented. Results were compared with that achieved by using nitrocellulose as a reference material. Possible interfering from the sample matrix on assay quality was investigated with spiked oral fluid samples. Under optimized conditions, a visually assessed limit of detection for the sandwich assay was 1 ng/mL, indicating that the paper-based test devices developed in this work can perform screening for drugs of abuse and can fulfill the requirement for a sensitive assay in diagnostically relevant ranges

AB - Morphine was used as a model analyte to examine the possibility of using cellulose, physically modified by papermaking and converting techniques, as a capillary matrix in a lateral flow type of diagnostic assay. This research was directed toward low-cost, disposable, and portable paperbased diagnostics, with the aim of addressing the analytical performance of paper as a substrate in the analysis for drugs of abuse. Antibody Fab fragments were used as sensing molecules, and gold nanoparticle detection was employed. Inkjet printing was used to pattern sensing biomolecules as detection zones on paper. To validate the usefulness of paper as a diagnostic platform, the principle of a direct sandwich assay, based on immunocomplex formation between morphine and the anti-morphine Fab fragment and detection of the formed immunocomplex by another Fab fragment, was implemented. Results were compared with that achieved by using nitrocellulose as a reference material. Possible interfering from the sample matrix on assay quality was investigated with spiked oral fluid samples. Under optimized conditions, a visually assessed limit of detection for the sandwich assay was 1 ng/mL, indicating that the paper-based test devices developed in this work can perform screening for drugs of abuse and can fulfill the requirement for a sensitive assay in diagnostically relevant ranges

KW - paper-based

KW - drugs of abuse

KW - recombinant antibody

KW - immunocomplex assay

KW - point-of-care diagnostics

KW - low resources

KW - capillary actions

KW - cellulose

U2 - 10.1007/s00216-014-8001-7

DO - 10.1007/s00216-014-8001-7

M3 - Article

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JO - Analytical and Bioanalytical Chemistry

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