A quantitative method for assessing bacteriocins and other food antimicrobials by automated turbidometry

Eija Skyttä (Corresponding Author), Tiina Mattila-Sandholm

Research output: Contribution to journalArticleScientificpeer-review

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Abstract

Automated turbidometry was used in assessing the antimicrobial efficacy of bacteriocin-like inhibitors produced by Pediococcus damnosus and Pediococcus pentosaceus. Four bacterial strains, i.e., Pseudomonas aeruginosa, Pseudomonas fragi, Salmonella enteritidis and Salmonella infantis were used as the test organisms. The growth of the test organisms was kinetically monitored throughout the incubation period and various growth curve parameters were used as quantitative indicators of growth inhibition. The results obtained in this study showed that a kinetic method based on automated turbidometry provides a sophisticated tool for the determination of bacteriocin activity. A number of growth curve parameters were applicable for the quantification of inhibitory effects. Regression analysis showed that the linear relationship between the level values and the log CFU counts was in most cases highly significant. However, the area value after 24 h appeared to describe most effectively the overall inhibitory effects since all the other growth curve parameters were simple end-point values giving information solely at a single point of time. Futhermore, the area reduction rate shown by the subsequent serial dilutions of the antimicrobials tested appeared to provide additional data on their antibacterial potency.

Original languageEnglish
Pages (from-to)77 - 88
Number of pages12
JournalJournal of Microbiological Methods
Volume14
Issue number2
DOIs
Publication statusPublished - 1991
MoE publication typeA1 Journal article-refereed

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Bacteriocins
Food
Growth
Pseudomonas fragi
Pediococcus
Salmonella enteritidis
Salmonella
Pseudomonas aeruginosa
Regression Analysis

Cite this

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title = "A quantitative method for assessing bacteriocins and other food antimicrobials by automated turbidometry",
abstract = "Automated turbidometry was used in assessing the antimicrobial efficacy of bacteriocin-like inhibitors produced by Pediococcus damnosus and Pediococcus pentosaceus. Four bacterial strains, i.e., Pseudomonas aeruginosa, Pseudomonas fragi, Salmonella enteritidis and Salmonella infantis were used as the test organisms. The growth of the test organisms was kinetically monitored throughout the incubation period and various growth curve parameters were used as quantitative indicators of growth inhibition. The results obtained in this study showed that a kinetic method based on automated turbidometry provides a sophisticated tool for the determination of bacteriocin activity. A number of growth curve parameters were applicable for the quantification of inhibitory effects. Regression analysis showed that the linear relationship between the level values and the log CFU counts was in most cases highly significant. However, the area value after 24 h appeared to describe most effectively the overall inhibitory effects since all the other growth curve parameters were simple end-point values giving information solely at a single point of time. Futhermore, the area reduction rate shown by the subsequent serial dilutions of the antimicrobials tested appeared to provide additional data on their antibacterial potency.",
author = "Eija Skytt{\"a} and Tiina Mattila-Sandholm",
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A quantitative method for assessing bacteriocins and other food antimicrobials by automated turbidometry. / Skyttä, Eija (Corresponding Author); Mattila-Sandholm, Tiina.

In: Journal of Microbiological Methods, Vol. 14, No. 2, 1991, p. 77 - 88.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - A quantitative method for assessing bacteriocins and other food antimicrobials by automated turbidometry

AU - Skyttä, Eija

AU - Mattila-Sandholm, Tiina

N1 - Project code: ELI8000 Project code: ELI12024

PY - 1991

Y1 - 1991

N2 - Automated turbidometry was used in assessing the antimicrobial efficacy of bacteriocin-like inhibitors produced by Pediococcus damnosus and Pediococcus pentosaceus. Four bacterial strains, i.e., Pseudomonas aeruginosa, Pseudomonas fragi, Salmonella enteritidis and Salmonella infantis were used as the test organisms. The growth of the test organisms was kinetically monitored throughout the incubation period and various growth curve parameters were used as quantitative indicators of growth inhibition. The results obtained in this study showed that a kinetic method based on automated turbidometry provides a sophisticated tool for the determination of bacteriocin activity. A number of growth curve parameters were applicable for the quantification of inhibitory effects. Regression analysis showed that the linear relationship between the level values and the log CFU counts was in most cases highly significant. However, the area value after 24 h appeared to describe most effectively the overall inhibitory effects since all the other growth curve parameters were simple end-point values giving information solely at a single point of time. Futhermore, the area reduction rate shown by the subsequent serial dilutions of the antimicrobials tested appeared to provide additional data on their antibacterial potency.

AB - Automated turbidometry was used in assessing the antimicrobial efficacy of bacteriocin-like inhibitors produced by Pediococcus damnosus and Pediococcus pentosaceus. Four bacterial strains, i.e., Pseudomonas aeruginosa, Pseudomonas fragi, Salmonella enteritidis and Salmonella infantis were used as the test organisms. The growth of the test organisms was kinetically monitored throughout the incubation period and various growth curve parameters were used as quantitative indicators of growth inhibition. The results obtained in this study showed that a kinetic method based on automated turbidometry provides a sophisticated tool for the determination of bacteriocin activity. A number of growth curve parameters were applicable for the quantification of inhibitory effects. Regression analysis showed that the linear relationship between the level values and the log CFU counts was in most cases highly significant. However, the area value after 24 h appeared to describe most effectively the overall inhibitory effects since all the other growth curve parameters were simple end-point values giving information solely at a single point of time. Futhermore, the area reduction rate shown by the subsequent serial dilutions of the antimicrobials tested appeared to provide additional data on their antibacterial potency.

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