A Sec1-related vesicle-transport protein that is expressed predominantly in epithelial cells

Kirsi Riento, Jussi Jäntti, Sanna Jansson, Sebastian Hielm, Eero Lehtonen, Christian Ehnholm, Sirkka Keränen, Vesa Olkkonen

Research output: Contribution to journalArticleScientificpeer-review

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Abstract

Sec1‐related proteins are involved in docking and fusion of transport vesicles in eukaryotic cells. Here we report the cloning and molecular characterization of a Sec1‐related protein expressed in the MDCK epithelial cell line. This protein represents a canine counterpart of the murine Munc‐18–2/Munc‐18b/muSec1 protein, displays 93% amino acid identity with these proteins, has a similar tissue mRNA expression pattern, and associates in vitro with syntaxins 1A, 2, and 3. In situ hybridization analysis of embryonic mouse tissues revealed prominent expression of the munc‐18‐2 mRNA in the epithelia of several tissues. Cell‐fractionation studies demonstrated that the majority of Munc‐18‐2 is membrane associated. Most of the protein is washed off the membranes by sodium carbonate, pH 11.5. However, the protein is poorly solubilized by detergent treatment. The Munc‐18‐2 protein was localized, by immunofluorescence microscopy, to the plasma membrane of MDCK cells, and is apically distributed in the epithelial cells of mouse tissues. When overexpressed in COS‐1 cells, the protein appeared to be largely cytosolic. However, upon expression with syntaxin 1A, it displayed a shift to the plasma membrane, where the two proteins colocalized. These results identified Munc‐18‐2 as a predominantly epithelial vesicle‐transport protein with a polarized distribution and provided novel in vivo evidence for the association of Sec1‐related proteins with members of the syntaxin family.
Original languageEnglish
Pages (from-to)638-646
Number of pages9
JournalEuropean Journal of Biochemistry
Volume239
Issue number3
DOIs
Publication statusPublished - 1996
MoE publication typeA1 Journal article-refereed

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Carrier Proteins
Epithelial Cells
Syntaxin 1
Proteins
Qa-SNARE Proteins
Madin Darby Canine Kidney Cells
Tissue
Cell membranes
Munc18 Proteins
Cell Membrane
Transport Vesicles
Messenger RNA
Membranes
Molecular Cloning
Eukaryotic Cells
Fluorescence Microscopy
Cloning
Detergents
In Situ Hybridization
Canidae

Cite this

Riento, Kirsi ; Jäntti, Jussi ; Jansson, Sanna ; Hielm, Sebastian ; Lehtonen, Eero ; Ehnholm, Christian ; Keränen, Sirkka ; Olkkonen, Vesa. / A Sec1-related vesicle-transport protein that is expressed predominantly in epithelial cells. In: European Journal of Biochemistry. 1996 ; Vol. 239, No. 3. pp. 638-646.
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title = "A Sec1-related vesicle-transport protein that is expressed predominantly in epithelial cells",
abstract = "Sec1‐related proteins are involved in docking and fusion of transport vesicles in eukaryotic cells. Here we report the cloning and molecular characterization of a Sec1‐related protein expressed in the MDCK epithelial cell line. This protein represents a canine counterpart of the murine Munc‐18–2/Munc‐18b/muSec1 protein, displays 93{\%} amino acid identity with these proteins, has a similar tissue mRNA expression pattern, and associates in vitro with syntaxins 1A, 2, and 3. In situ hybridization analysis of embryonic mouse tissues revealed prominent expression of the munc‐18‐2 mRNA in the epithelia of several tissues. Cell‐fractionation studies demonstrated that the majority of Munc‐18‐2 is membrane associated. Most of the protein is washed off the membranes by sodium carbonate, pH 11.5. However, the protein is poorly solubilized by detergent treatment. The Munc‐18‐2 protein was localized, by immunofluorescence microscopy, to the plasma membrane of MDCK cells, and is apically distributed in the epithelial cells of mouse tissues. When overexpressed in COS‐1 cells, the protein appeared to be largely cytosolic. However, upon expression with syntaxin 1A, it displayed a shift to the plasma membrane, where the two proteins colocalized. These results identified Munc‐18‐2 as a predominantly epithelial vesicle‐transport protein with a polarized distribution and provided novel in vivo evidence for the association of Sec1‐related proteins with members of the syntaxin family.",
author = "Kirsi Riento and Jussi J{\"a}ntti and Sanna Jansson and Sebastian Hielm and Eero Lehtonen and Christian Ehnholm and Sirkka Ker{\"a}nen and Vesa Olkkonen",
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Riento, K, Jäntti, J, Jansson, S, Hielm, S, Lehtonen, E, Ehnholm, C, Keränen, S & Olkkonen, V 1996, 'A Sec1-related vesicle-transport protein that is expressed predominantly in epithelial cells', European Journal of Biochemistry, vol. 239, no. 3, pp. 638-646. https://doi.org/10.1111/j.1432-1033.1996.0638u.x

A Sec1-related vesicle-transport protein that is expressed predominantly in epithelial cells. / Riento, Kirsi; Jäntti, Jussi; Jansson, Sanna; Hielm, Sebastian; Lehtonen, Eero; Ehnholm, Christian; Keränen, Sirkka; Olkkonen, Vesa.

In: European Journal of Biochemistry, Vol. 239, No. 3, 1996, p. 638-646.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - A Sec1-related vesicle-transport protein that is expressed predominantly in epithelial cells

AU - Riento, Kirsi

AU - Jäntti, Jussi

AU - Jansson, Sanna

AU - Hielm, Sebastian

AU - Lehtonen, Eero

AU - Ehnholm, Christian

AU - Keränen, Sirkka

AU - Olkkonen, Vesa

N1 - Project code: B6SU00051

PY - 1996

Y1 - 1996

N2 - Sec1‐related proteins are involved in docking and fusion of transport vesicles in eukaryotic cells. Here we report the cloning and molecular characterization of a Sec1‐related protein expressed in the MDCK epithelial cell line. This protein represents a canine counterpart of the murine Munc‐18–2/Munc‐18b/muSec1 protein, displays 93% amino acid identity with these proteins, has a similar tissue mRNA expression pattern, and associates in vitro with syntaxins 1A, 2, and 3. In situ hybridization analysis of embryonic mouse tissues revealed prominent expression of the munc‐18‐2 mRNA in the epithelia of several tissues. Cell‐fractionation studies demonstrated that the majority of Munc‐18‐2 is membrane associated. Most of the protein is washed off the membranes by sodium carbonate, pH 11.5. However, the protein is poorly solubilized by detergent treatment. The Munc‐18‐2 protein was localized, by immunofluorescence microscopy, to the plasma membrane of MDCK cells, and is apically distributed in the epithelial cells of mouse tissues. When overexpressed in COS‐1 cells, the protein appeared to be largely cytosolic. However, upon expression with syntaxin 1A, it displayed a shift to the plasma membrane, where the two proteins colocalized. These results identified Munc‐18‐2 as a predominantly epithelial vesicle‐transport protein with a polarized distribution and provided novel in vivo evidence for the association of Sec1‐related proteins with members of the syntaxin family.

AB - Sec1‐related proteins are involved in docking and fusion of transport vesicles in eukaryotic cells. Here we report the cloning and molecular characterization of a Sec1‐related protein expressed in the MDCK epithelial cell line. This protein represents a canine counterpart of the murine Munc‐18–2/Munc‐18b/muSec1 protein, displays 93% amino acid identity with these proteins, has a similar tissue mRNA expression pattern, and associates in vitro with syntaxins 1A, 2, and 3. In situ hybridization analysis of embryonic mouse tissues revealed prominent expression of the munc‐18‐2 mRNA in the epithelia of several tissues. Cell‐fractionation studies demonstrated that the majority of Munc‐18‐2 is membrane associated. Most of the protein is washed off the membranes by sodium carbonate, pH 11.5. However, the protein is poorly solubilized by detergent treatment. The Munc‐18‐2 protein was localized, by immunofluorescence microscopy, to the plasma membrane of MDCK cells, and is apically distributed in the epithelial cells of mouse tissues. When overexpressed in COS‐1 cells, the protein appeared to be largely cytosolic. However, upon expression with syntaxin 1A, it displayed a shift to the plasma membrane, where the two proteins colocalized. These results identified Munc‐18‐2 as a predominantly epithelial vesicle‐transport protein with a polarized distribution and provided novel in vivo evidence for the association of Sec1‐related proteins with members of the syntaxin family.

U2 - 10.1111/j.1432-1033.1996.0638u.x

DO - 10.1111/j.1432-1033.1996.0638u.x

M3 - Article

VL - 239

SP - 638

EP - 646

JO - FEBS Journal

JF - FEBS Journal

SN - 1742-464X

IS - 3

ER -