A short-chain dehydrogenase gene from Pichia stipitis having D-arabinitol dehydrogenase activity

Johan Hallborn; Mats Walfridsson; Merja Penttilä; Sirkka Keränen; Bärbel Hahn-Hägerdal

doi:10.1002/yea.320110906

A short-chain dehydrogenase gene from Pichia stipitis having D-arabinitol dehydrogenase activity

Johan Hallborn
, Mats Walfridsson
, Merja Penttilä
, Sirkka Keränen
, Bärbel Hahn-Hägerdal^*

^*Corresponding author for this work

Lund University

Research output: Contribution to journal › Article › Scientific › peer-review

22 Citations (Scopus)

Abstract

An NAD⁺-dependent D-arabinitol dehydrogenase (polyol dehydrogenase) gene was isolated from Pichia stipitis CBS 6054 and cloned in Saccharomyces cerevisiae. The gene was isolated by screening of a λ-cDNA library with a zymogram technique. D-Arabinitol, xylitol, D-glucitol and galactitol are substrates for the recominant protein. With D-arabinitol as substrate the reaction product is D-ribulose. The molecular weight of the native tetramer enzyme is 110 000 Da and the monomer is 30 000 Da. The amino acid sequence is homologous to the short-chain dehydrogenase family. It is 85·5% identical to a D-arabinitol dehydrogenase from Candida albicans. The gene in P. stipitis was induced by D-arabinitol and P. stipitis was able to grow on D-arabinitol. The physiological role of D-arabinitol metabolism is discussed.

Original language	English
Pages (from-to)	839-847
Journal	Yeast
Volume	11
Issue number	9
DOIs	https://doi.org/10.1002/yea.320110906
Publication status	Published - 1 Jan 1995
MoE publication type	A1 Journal article-refereed

Keywords

arabinitol metabolism
ARDH
D-arabinitol dehydrogenase
overexpression
Pichia stipitis
Saccharomyces cerevisiae
xylose metabolism
yeast
zymogram screening

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title = "A short-chain dehydrogenase gene from Pichia stipitis having D-arabinitol dehydrogenase activity",

abstract = "An NAD+-dependent D-arabinitol dehydrogenase (polyol dehydrogenase) gene was isolated from Pichia stipitis CBS 6054 and cloned in Saccharomyces cerevisiae. The gene was isolated by screening of a λ-cDNA library with a zymogram technique. D-Arabinitol, xylitol, D-glucitol and galactitol are substrates for the recominant protein. With D-arabinitol as substrate the reaction product is D-ribulose. The molecular weight of the native tetramer enzyme is 110 000 Da and the monomer is 30 000 Da. The amino acid sequence is homologous to the short-chain dehydrogenase family. It is 85·5\% identical to a D-arabinitol dehydrogenase from Candida albicans. The gene in P. stipitis was induced by D-arabinitol and P. stipitis was able to grow on D-arabinitol. The physiological role of D-arabinitol metabolism is discussed.",

keywords = "arabinitol metabolism, ARDH, D-arabinitol dehydrogenase, overexpression, Pichia stipitis, Saccharomyces cerevisiae, xylose metabolism, yeast, zymogram screening",

author = "Johan Hallborn and Mats Walfridsson and Merja Penttil{\"a} and Sirkka Ker{\"a}nen and B{\"a}rbel Hahn-H{\"a}gerdal",

year = "1995",

month = jan,

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doi = "10.1002/yea.320110906",

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T1 - A short-chain dehydrogenase gene from Pichia stipitis having D-arabinitol dehydrogenase activity

AU - Hallborn, Johan

AU - Walfridsson, Mats

AU - Penttilä, Merja

AU - Keränen, Sirkka

AU - Hahn-Hägerdal, Bärbel

PY - 1995/1/1

Y1 - 1995/1/1

N2 - An NAD+-dependent D-arabinitol dehydrogenase (polyol dehydrogenase) gene was isolated from Pichia stipitis CBS 6054 and cloned in Saccharomyces cerevisiae. The gene was isolated by screening of a λ-cDNA library with a zymogram technique. D-Arabinitol, xylitol, D-glucitol and galactitol are substrates for the recominant protein. With D-arabinitol as substrate the reaction product is D-ribulose. The molecular weight of the native tetramer enzyme is 110 000 Da and the monomer is 30 000 Da. The amino acid sequence is homologous to the short-chain dehydrogenase family. It is 85·5% identical to a D-arabinitol dehydrogenase from Candida albicans. The gene in P. stipitis was induced by D-arabinitol and P. stipitis was able to grow on D-arabinitol. The physiological role of D-arabinitol metabolism is discussed.

AB - An NAD+-dependent D-arabinitol dehydrogenase (polyol dehydrogenase) gene was isolated from Pichia stipitis CBS 6054 and cloned in Saccharomyces cerevisiae. The gene was isolated by screening of a λ-cDNA library with a zymogram technique. D-Arabinitol, xylitol, D-glucitol and galactitol are substrates for the recominant protein. With D-arabinitol as substrate the reaction product is D-ribulose. The molecular weight of the native tetramer enzyme is 110 000 Da and the monomer is 30 000 Da. The amino acid sequence is homologous to the short-chain dehydrogenase family. It is 85·5% identical to a D-arabinitol dehydrogenase from Candida albicans. The gene in P. stipitis was induced by D-arabinitol and P. stipitis was able to grow on D-arabinitol. The physiological role of D-arabinitol metabolism is discussed.

KW - arabinitol metabolism

KW - ARDH

KW - D-arabinitol dehydrogenase

KW - overexpression

KW - Pichia stipitis

KW - Saccharomyces cerevisiae

KW - xylose metabolism

KW - yeast

KW - zymogram screening

UR - https://www.scopus.com/pages/publications/0029013247

U2 - 10.1002/yea.320110906

DO - 10.1002/yea.320110906

M3 - Article

C2 - 7483848

AN - SCOPUS:0029013247

SN - 0749-503X

VL - 11

SP - 839

EP - 847

JO - Yeast

JF - Yeast

IS - 9

ER -

A short-chain dehydrogenase gene from Pichia stipitis having D-arabinitol dehydrogenase activity

Abstract

Keywords

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