A staining technique to measure capsular polysaccharides of Staphylococcus aureus on filter membranes

Tiina Mattila

Research output: Contribution to journalArticleScientificpeer-review

3 Citations (Scopus)

Abstract

Three strains of Staphylococcus aureus indicating diffuse growth in serum-soft agar and three strains of S. aureus growing as compact colonies were grown on cellulose-acetate membranes laid on trypticase soy and modified 110 agar plates.
The production of capsule material on the membrane filters was detected by staining with ruthenium-red. The intensity and color indexes of the reflected light was analysed using a chroma detector interfaced to a minicomputer.
The intensity of the reflected light and the red color index showed marked differences between the diffuse and compact staphylococci, indicating that the staining method is useful for studying bacterial capsule or slime formation. Treatment with egg-white lysozyme destroyed some slime layers of the compact colony-forming staphylococci whereas the diffuse colony-forming bacteria remained intact on the membranes.
The technique is of special value in studying the means to destroy capsular material as well as in comparing different microbes within individual species with respect to their formation of capsular material or slime.
Original languageEnglish
Pages (from-to)323-331
JournalJournal of Microbiological Methods
Volume9
Issue number4
DOIs
Publication statusPublished - 1989
MoE publication typeA1 Journal article-refereed

Fingerprint

Polysaccharides
Staphylococcus aureus
Staining and Labeling
Staphylococcus
Agar
Membranes
Bacterial Capsules
Color
Minicomputers
Light
Ruthenium Red
Egg White
Muramidase
Capsules
Bacteria
Growth
Serum
trypticase-soy broth
acetylcellulose

Cite this

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title = "A staining technique to measure capsular polysaccharides of Staphylococcus aureus on filter membranes",
abstract = "Three strains of Staphylococcus aureus indicating diffuse growth in serum-soft agar and three strains of S. aureus growing as compact colonies were grown on cellulose-acetate membranes laid on trypticase soy and modified 110 agar plates. The production of capsule material on the membrane filters was detected by staining with ruthenium-red. The intensity and color indexes of the reflected light was analysed using a chroma detector interfaced to a minicomputer. The intensity of the reflected light and the red color index showed marked differences between the diffuse and compact staphylococci, indicating that the staining method is useful for studying bacterial capsule or slime formation. Treatment with egg-white lysozyme destroyed some slime layers of the compact colony-forming staphylococci whereas the diffuse colony-forming bacteria remained intact on the membranes. The technique is of special value in studying the means to destroy capsular material as well as in comparing different microbes within individual species with respect to their formation of capsular material or slime.",
author = "Tiina Mattila",
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A staining technique to measure capsular polysaccharides of Staphylococcus aureus on filter membranes. / Mattila, Tiina.

In: Journal of Microbiological Methods, Vol. 9, No. 4, 1989, p. 323-331.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - A staining technique to measure capsular polysaccharides of Staphylococcus aureus on filter membranes

AU - Mattila, Tiina

PY - 1989

Y1 - 1989

N2 - Three strains of Staphylococcus aureus indicating diffuse growth in serum-soft agar and three strains of S. aureus growing as compact colonies were grown on cellulose-acetate membranes laid on trypticase soy and modified 110 agar plates. The production of capsule material on the membrane filters was detected by staining with ruthenium-red. The intensity and color indexes of the reflected light was analysed using a chroma detector interfaced to a minicomputer. The intensity of the reflected light and the red color index showed marked differences between the diffuse and compact staphylococci, indicating that the staining method is useful for studying bacterial capsule or slime formation. Treatment with egg-white lysozyme destroyed some slime layers of the compact colony-forming staphylococci whereas the diffuse colony-forming bacteria remained intact on the membranes. The technique is of special value in studying the means to destroy capsular material as well as in comparing different microbes within individual species with respect to their formation of capsular material or slime.

AB - Three strains of Staphylococcus aureus indicating diffuse growth in serum-soft agar and three strains of S. aureus growing as compact colonies were grown on cellulose-acetate membranes laid on trypticase soy and modified 110 agar plates. The production of capsule material on the membrane filters was detected by staining with ruthenium-red. The intensity and color indexes of the reflected light was analysed using a chroma detector interfaced to a minicomputer. The intensity of the reflected light and the red color index showed marked differences between the diffuse and compact staphylococci, indicating that the staining method is useful for studying bacterial capsule or slime formation. Treatment with egg-white lysozyme destroyed some slime layers of the compact colony-forming staphylococci whereas the diffuse colony-forming bacteria remained intact on the membranes. The technique is of special value in studying the means to destroy capsular material as well as in comparing different microbes within individual species with respect to their formation of capsular material or slime.

U2 - 10.1016/0167-7012(89)90074-2

DO - 10.1016/0167-7012(89)90074-2

M3 - Article

VL - 9

SP - 323

EP - 331

JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

SN - 0167-7012

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