A streptavidin-luciferase fusion proteins: Comparisons and applications

Matti Karp (Corresponding Author), Christian Oker-Blom

Research output: Contribution to journalArticleScientificpeer-review

18 Citations (Scopus)

Abstract

Luciferases are unique enzymes in being capable of emitting visible light as one of the end-products of their catalysis. Both procaryotic and eucaryotic organisms exist that emit light, and the luciferases from these organisms differ considerably in size as well as chemistry of catalysis. Two main, i.e. most studied groups, are the bacterial luciferases of e.g. Vibrio fisheri, Vibrio harveyi, and Photorhabdus luminescens, responding to FMNH2, long-chain aldehyde and molecular oxygen and the insect luciferases of the fireflies Photinus pyralis and Luciola minengrelica or click beetle Pyrophorus plagiophthalamus, responding to ATP, luciferin and molecular oxygen. An emerging amount of ‘new’ luciferases from shrimps, fish, jelly fish and overall from marine origin, are finding their way to biotechnological applications. The common feature of these is their ability to produce light within the visible region of the spectrum, i.e. between 450 nm (blue) and 630 nm (red). In this short review, we discuss some of the recent advances on fusion proteins of eucaryotic luciferases and their applications. Special emphasis is placed on a streptavidin–luciferase fusion protein produced by insect cells using the baculovirus expression system.
Original languageEnglish
Pages (from-to)101-104
Number of pages4
JournalBiomolecular Engineering
Volume16
Issue number1-4
DOIs
Publication statusPublished - 1999
MoE publication typeA1 Journal article-refereed

Fingerprint

Streptavidin
Luciferases
Fusion reactions
Molecular oxygen
Proteins
Fish
Catalysis
Vibrio
Light
Fishes
Photorhabdus
Bacterial Luciferases
Fireflies
Adenosinetriphosphate
Insect Proteins
Oxygen
Aldehydes
Firefly Luciferases
Baculoviridae
Beetles

Cite this

Karp, Matti ; Oker-Blom, Christian. / A streptavidin-luciferase fusion proteins : Comparisons and applications. In: Biomolecular Engineering. 1999 ; Vol. 16, No. 1-4. pp. 101-104.
@article{06a84f86ae0f40e48efbafdc50829e53,
title = "A streptavidin-luciferase fusion proteins: Comparisons and applications",
abstract = "Luciferases are unique enzymes in being capable of emitting visible light as one of the end-products of their catalysis. Both procaryotic and eucaryotic organisms exist that emit light, and the luciferases from these organisms differ considerably in size as well as chemistry of catalysis. Two main, i.e. most studied groups, are the bacterial luciferases of e.g. Vibrio fisheri, Vibrio harveyi, and Photorhabdus luminescens, responding to FMNH2, long-chain aldehyde and molecular oxygen and the insect luciferases of the fireflies Photinus pyralis and Luciola minengrelica or click beetle Pyrophorus plagiophthalamus, responding to ATP, luciferin and molecular oxygen. An emerging amount of ‘new’ luciferases from shrimps, fish, jelly fish and overall from marine origin, are finding their way to biotechnological applications. The common feature of these is their ability to produce light within the visible region of the spectrum, i.e. between 450 nm (blue) and 630 nm (red). In this short review, we discuss some of the recent advances on fusion proteins of eucaryotic luciferases and their applications. Special emphasis is placed on a streptavidin–luciferase fusion protein produced by insect cells using the baculovirus expression system.",
author = "Matti Karp and Christian Oker-Blom",
year = "1999",
doi = "10.1016/S1050-3862(99)00039-X",
language = "English",
volume = "16",
pages = "101--104",
journal = "New Biotechnology",
issn = "1871-6784",
publisher = "Elsevier",
number = "1-4",

}

A streptavidin-luciferase fusion proteins : Comparisons and applications. / Karp, Matti (Corresponding Author); Oker-Blom, Christian.

In: Biomolecular Engineering, Vol. 16, No. 1-4, 1999, p. 101-104.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - A streptavidin-luciferase fusion proteins

T2 - Comparisons and applications

AU - Karp, Matti

AU - Oker-Blom, Christian

PY - 1999

Y1 - 1999

N2 - Luciferases are unique enzymes in being capable of emitting visible light as one of the end-products of their catalysis. Both procaryotic and eucaryotic organisms exist that emit light, and the luciferases from these organisms differ considerably in size as well as chemistry of catalysis. Two main, i.e. most studied groups, are the bacterial luciferases of e.g. Vibrio fisheri, Vibrio harveyi, and Photorhabdus luminescens, responding to FMNH2, long-chain aldehyde and molecular oxygen and the insect luciferases of the fireflies Photinus pyralis and Luciola minengrelica or click beetle Pyrophorus plagiophthalamus, responding to ATP, luciferin and molecular oxygen. An emerging amount of ‘new’ luciferases from shrimps, fish, jelly fish and overall from marine origin, are finding their way to biotechnological applications. The common feature of these is their ability to produce light within the visible region of the spectrum, i.e. between 450 nm (blue) and 630 nm (red). In this short review, we discuss some of the recent advances on fusion proteins of eucaryotic luciferases and their applications. Special emphasis is placed on a streptavidin–luciferase fusion protein produced by insect cells using the baculovirus expression system.

AB - Luciferases are unique enzymes in being capable of emitting visible light as one of the end-products of their catalysis. Both procaryotic and eucaryotic organisms exist that emit light, and the luciferases from these organisms differ considerably in size as well as chemistry of catalysis. Two main, i.e. most studied groups, are the bacterial luciferases of e.g. Vibrio fisheri, Vibrio harveyi, and Photorhabdus luminescens, responding to FMNH2, long-chain aldehyde and molecular oxygen and the insect luciferases of the fireflies Photinus pyralis and Luciola minengrelica or click beetle Pyrophorus plagiophthalamus, responding to ATP, luciferin and molecular oxygen. An emerging amount of ‘new’ luciferases from shrimps, fish, jelly fish and overall from marine origin, are finding their way to biotechnological applications. The common feature of these is their ability to produce light within the visible region of the spectrum, i.e. between 450 nm (blue) and 630 nm (red). In this short review, we discuss some of the recent advances on fusion proteins of eucaryotic luciferases and their applications. Special emphasis is placed on a streptavidin–luciferase fusion protein produced by insect cells using the baculovirus expression system.

U2 - 10.1016/S1050-3862(99)00039-X

DO - 10.1016/S1050-3862(99)00039-X

M3 - Article

VL - 16

SP - 101

EP - 104

JO - New Biotechnology

JF - New Biotechnology

SN - 1871-6784

IS - 1-4

ER -