A survey of carbonic anhydrase mRNA expression in enamel cells

Rodrigo S. Lacruz, Mika Hilvo, Ira Kurtz, Michael L. Paine (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

22 Citations (Scopus)

Abstract

Enamel formation requires rigid control of pH homeostasis during all stages of development to prevent disruptions to crystal growth. The acceleration of the generation of bicarbonate by carbonic anhydrases (CA) has been suggested as one of the pathways used by ameloblasts cells to regulate extracellular pH yet only two isozymes (CA II and CA VI) have been reported to date during enamel formation. The mammalian CA family contains 16 different isoforms of which 13 are enzymatically active. We have conducted a systematic screening by RT-PCR on the expression of all known CA isoforms in mouse enamel organ epithelium (EOE) cells dissected from new born, in secretory ameloblasts derived from 7-day-old animals, and in the LS8 ameloblast cell line. Results show that all CA isoforms are expressed by EOE/ameloblast cells in vivo. The most highly expressed are the catalytic isozymes CA II, VI, IX, and XIII, and the acatalytic CA XI isoform. Only minor differences were found in CA expression levels between 1-day EOE cells and 7-day-old secretory-stage ameloblasts, whereas LS8 cells expressed fewer CA isoforms than both of these. The broad expression of CAs by ameloblasts reported here contributes to our understanding of pH homeostasis during enamel development and demonstrates its complexity. Our results also highlight the critical role that regulation of pH plays during the development of enamel.
Original languageEnglish
Pages (from-to)883-887
JournalBiochemical and Biophysical Research Communications
Volume393
Issue number4
DOIs
Publication statusPublished - 2010
MoE publication typeA1 Journal article-refereed

Fingerprint

Ameloblasts
Carbonic Anhydrases
Enamels
Dental Enamel
Enamel Organ
Messenger RNA
Protein Isoforms
Carbonic Anhydrase II
Epithelium
Isoenzymes
Homeostasis
Bicarbonates
Crystallization
Surveys and Questionnaires
Screening
Animals
Cells
Cell Line
Polymerase Chain Reaction

Keywords

  • Acid/base transport
  • Ameloblast
  • Carbonic anhydrase
  • Enamel

Cite this

Lacruz, Rodrigo S. ; Hilvo, Mika ; Kurtz, Ira ; Paine, Michael L. / A survey of carbonic anhydrase mRNA expression in enamel cells. In: Biochemical and Biophysical Research Communications. 2010 ; Vol. 393, No. 4. pp. 883-887.
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A survey of carbonic anhydrase mRNA expression in enamel cells. / Lacruz, Rodrigo S.; Hilvo, Mika; Kurtz, Ira; Paine, Michael L. (Corresponding Author).

In: Biochemical and Biophysical Research Communications, Vol. 393, No. 4, 2010, p. 883-887.

Research output: Contribution to journalArticleScientificpeer-review

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AU - Paine, Michael L.

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N2 - Enamel formation requires rigid control of pH homeostasis during all stages of development to prevent disruptions to crystal growth. The acceleration of the generation of bicarbonate by carbonic anhydrases (CA) has been suggested as one of the pathways used by ameloblasts cells to regulate extracellular pH yet only two isozymes (CA II and CA VI) have been reported to date during enamel formation. The mammalian CA family contains 16 different isoforms of which 13 are enzymatically active. We have conducted a systematic screening by RT-PCR on the expression of all known CA isoforms in mouse enamel organ epithelium (EOE) cells dissected from new born, in secretory ameloblasts derived from 7-day-old animals, and in the LS8 ameloblast cell line. Results show that all CA isoforms are expressed by EOE/ameloblast cells in vivo. The most highly expressed are the catalytic isozymes CA II, VI, IX, and XIII, and the acatalytic CA XI isoform. Only minor differences were found in CA expression levels between 1-day EOE cells and 7-day-old secretory-stage ameloblasts, whereas LS8 cells expressed fewer CA isoforms than both of these. The broad expression of CAs by ameloblasts reported here contributes to our understanding of pH homeostasis during enamel development and demonstrates its complexity. Our results also highlight the critical role that regulation of pH plays during the development of enamel.

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