A Universal Gene Expression System for Novel Yeast Species

Dominik Mojzita (Corresponding author), Anssi Rantasalo, Marja Ilmén

Research output: Chapter in Book/Report/Conference proceedingChapter or book articleScientificpeer-review

Abstract

The current progress in sequencing of genomes and characterization of new species provides an increasing list of yeasts that show interesting physiological properties; however, the lack of expression tools for these new hosts is prohibiting their broader use in research or industry. Recently, we developed a universal expression system (SES) functional in broad spectrum of fungal species, which represent a solution for feasible gene expression control and genetic manipulation in these novel hosts. Here, we describe three example approaches for DNA transformation and high-level heterologous gene expression, using the SES system, in three yeast species, where minimal knowledge or prior experience in genetic modifications is available.

Original languageEnglish
Title of host publicationYeast Metabolic Engineerin
EditorsV. Mapelli, M. Bettiga
PublisherHumana Press
Pages221-242
Number of pages22
ISBN (Electronic)978-1-0716-2399-2
ISBN (Print)978-1-0716-2398-5
DOIs
Publication statusPublished - 2022
MoE publication typeA3 Part of a book or another research book

Publication series

SeriesMethods in Molecular Biology
Volume2513
ISSN1064-3745

Keywords

  • Genetic tools
  • Heterologous gene expression
  • Metabolic engineering
  • Novel yeast species
  • Protein production
  • Synthetic expression system (SES)

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