A Universal Gene Expression System for Novel Yeast Species

Dominik Mojzita; Anssi Rantasalo; Marja Ilmén

doi:10.1007/978-1-0716-2399-2_13

A Universal Gene Expression System for Novel Yeast Species

Dominik Mojzita^*
, Anssi Rantasalo
, Marja Ilmén

^*Corresponding author for this work

BA5301 Production host engineering

eniferBio Oy

Research output: Chapter in Book/Report/Conference proceeding › Chapter or book article › Scientific › peer-review

2 Citations (Scopus)

Abstract

The current progress in sequencing of genomes and characterization of new species provides an increasing list of yeasts that show interesting physiological properties; however, the lack of expression tools for these new hosts is prohibiting their broader use in research or industry. Recently, we developed a universal expression system (SES) functional in broad spectrum of fungal species, which represent a solution for feasible gene expression control and genetic manipulation in these novel hosts. Here, we describe three example approaches for DNA transformation and high-level heterologous gene expression, using the SES system, in three yeast species, where minimal knowledge or prior experience in genetic modifications is available.

Original language	English
Title of host publication	Yeast Metabolic Engineering
Editors	V. Mapelli, M. Bettiga
Publisher	Humana Press
Pages	221-242
ISBN (Electronic)	978-1-0716-2399-2
ISBN (Print)	978-1-0716-2398-5
DOIs	https://doi.org/10.1007/978-1-0716-2399-2_13
Publication status	Published - 2022
MoE publication type	A3 Part of a book or another research book

Publication series

Series	Methods in Molecular Biology
Volume	2513
ISSN	1064-3745

Funding

This material is based upon work supported by the Finnish Funding Agency for Innovation (living factories project), and the Academy of Finland (MODUBIO, project 260957; SynBioPol, project 272550). Synthetic expression system (SES) has been granted a patent (Finnish patent (FI127283B) March 15, 2018) and is the subject of patent applications (PCT/FI2017/050114 on February 21, 2017 and 015234 US on December 18, 2017).

Keywords

Genetic tools
Heterologous gene expression
Metabolic engineering
Novel yeast species
Protein production
Synthetic expression system (SES)

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10.1007/978-1-0716-2399-2_13

Cite this

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AU - Rantasalo, Anssi

AU - Ilmén, Marja

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AB - The current progress in sequencing of genomes and characterization of new species provides an increasing list of yeasts that show interesting physiological properties; however, the lack of expression tools for these new hosts is prohibiting their broader use in research or industry. Recently, we developed a universal expression system (SES) functional in broad spectrum of fungal species, which represent a solution for feasible gene expression control and genetic manipulation in these novel hosts. Here, we describe three example approaches for DNA transformation and high-level heterologous gene expression, using the SES system, in three yeast species, where minimal knowledge or prior experience in genetic modifications is available.

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KW - Metabolic engineering

KW - Novel yeast species

KW - Protein production

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ER -

A Universal Gene Expression System for Novel Yeast Species

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