A Universal Gene Expression System for Novel Yeast Species

Dominik Mojzita; Anssi Rantasalo; Marja Ilmén

doi:10.1007/978-1-0716-2399-2_13

A Universal Gene Expression System for Novel Yeast Species

Dominik Mojzita^*
, Anssi Rantasalo
, Marja Ilmén

^*Corresponding author for this work

eniferBio Oy

Research output: Chapter in Book/Report/Conference proceeding › Chapter or book article › Scientific › peer-review

2 Citations (Scopus)

Abstract

The current progress in sequencing of genomes and characterization of new species provides an increasing list of yeasts that show interesting physiological properties; however, the lack of expression tools for these new hosts is prohibiting their broader use in research or industry. Recently, we developed a universal expression system (SES) functional in broad spectrum of fungal species, which represent a solution for feasible gene expression control and genetic manipulation in these novel hosts. Here, we describe three example approaches for DNA transformation and high-level heterologous gene expression, using the SES system, in three yeast species, where minimal knowledge or prior experience in genetic modifications is available.

Original language	English
Title of host publication	Yeast Metabolic Engineering
Editors	V. Mapelli, M. Bettiga
Publisher	Humana Press
Pages	221-242
ISBN (Electronic)	978-1-0716-2399-2
ISBN (Print)	978-1-0716-2398-5
DOIs	https://doi.org/10.1007/978-1-0716-2399-2_13
Publication status	Published - 2022
MoE publication type	A3 Part of a book or another research book

Publication series

Series	Methods in Molecular Biology
Volume	2513
ISSN	1064-3745

Funding

This material is based upon work supported by the Finnish Funding Agency for Innovation (living factories project), and the Academy of Finland (MODUBIO, project 260957; SynBioPol, project 272550). Synthetic expression system (SES) has been granted a patent (Finnish patent (FI127283B) March 15, 2018) and is the subject of patent applications (PCT/FI2017/050114 on February 21, 2017 and 015234 US on December 18, 2017).

Keywords

Genetic tools
Heterologous gene expression
Metabolic engineering
Novel yeast species
Protein production
Synthetic expression system (SES)

Access to Document

10.1007/978-1-0716-2399-2_13

Cite this

@inbook{0ec14b84061d4f30838a19b97cea8470,

title = "A Universal Gene Expression System for Novel Yeast Species",

abstract = "The current progress in sequencing of genomes and characterization of new species provides an increasing list of yeasts that show interesting physiological properties; however, the lack of expression tools for these new hosts is prohibiting their broader use in research or industry. Recently, we developed a universal expression system (SES) functional in broad spectrum of fungal species, which represent a solution for feasible gene expression control and genetic manipulation in these novel hosts. Here, we describe three example approaches for DNA transformation and high-level heterologous gene expression, using the SES system, in three yeast species, where minimal knowledge or prior experience in genetic modifications is available.",

keywords = "Genetic tools, Heterologous gene expression, Metabolic engineering, Novel yeast species, Protein production, Synthetic expression system (SES)",

author = "Dominik Mojzita and Anssi Rantasalo and Marja Ilm{\'e}n",

note = "Publisher Copyright: {\textcopyright} 2022, Springer Science+Business Media, LLC, part of Springer Nature.",

year = "2022",

doi = "10.1007/978-1-0716-2399-2\_13",

language = "English",

isbn = "978-1-0716-2398-5",

series = "Methods in Molecular Biology",

publisher = "Humana Press",

pages = "221--242",

editor = "V. Mapelli and M. Bettiga",

booktitle = "Yeast Metabolic Engineering",

address = "Germany",

}

TY - CHAP

T1 - A Universal Gene Expression System for Novel Yeast Species

AU - Mojzita, Dominik

AU - Rantasalo, Anssi

AU - Ilmén, Marja

PY - 2022

Y1 - 2022

N2 - The current progress in sequencing of genomes and characterization of new species provides an increasing list of yeasts that show interesting physiological properties; however, the lack of expression tools for these new hosts is prohibiting their broader use in research or industry. Recently, we developed a universal expression system (SES) functional in broad spectrum of fungal species, which represent a solution for feasible gene expression control and genetic manipulation in these novel hosts. Here, we describe three example approaches for DNA transformation and high-level heterologous gene expression, using the SES system, in three yeast species, where minimal knowledge or prior experience in genetic modifications is available.

AB - The current progress in sequencing of genomes and characterization of new species provides an increasing list of yeasts that show interesting physiological properties; however, the lack of expression tools for these new hosts is prohibiting their broader use in research or industry. Recently, we developed a universal expression system (SES) functional in broad spectrum of fungal species, which represent a solution for feasible gene expression control and genetic manipulation in these novel hosts. Here, we describe three example approaches for DNA transformation and high-level heterologous gene expression, using the SES system, in three yeast species, where minimal knowledge or prior experience in genetic modifications is available.

KW - Genetic tools

KW - Heterologous gene expression

KW - Metabolic engineering

KW - Novel yeast species

KW - Protein production

KW - Synthetic expression system (SES)

UR - https://www.scopus.com/pages/publications/85133272660

U2 - 10.1007/978-1-0716-2399-2_13

DO - 10.1007/978-1-0716-2399-2_13

M3 - Chapter or book article

C2 - 35781208

AN - SCOPUS:85133272660

SN - 978-1-0716-2398-5

T3 - Methods in Molecular Biology

SP - 221

EP - 242

BT - Yeast Metabolic Engineering

A2 - Mapelli, V.

A2 - Bettiga, M.

PB - Humana Press

ER -

A Universal Gene Expression System for Novel Yeast Species

Abstract

Publication series

Funding

Keywords

Access to Document

Other files and links

Fingerprint

Cite this