A versatile transformation system for the cellulolytic filamentous fungus Trichoderma reesei

Merja Penttilä; Helena Nevalainen; Marjaana Rättö; Elina Salminen; Jonathan Knowles

doi:10.1016/0378-1119(87)90110-7

A versatile transformation system for the cellulolytic filamentous fungus Trichoderma reesei

Merja Penttilä, Helena Nevalainen, Marjaana Rättö, Elina Salminen, Jonathan Knowles

University of Jyväskylä

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Abstract

An efficient transformation system for the cellulolytic filamentous fungus Trichoderma reesei has been developed. Transformation was obtained with plasmid carrying the dominant selectable marker amdS or the argB gene of Aspergillus nidulans, which was found to complement the respective argB mutation of T. reesei. The transformation frequency can be up to 600 transformants per μg of transforming DNA. The efficiency of co-transformation with unselected DNA was high (approx. 80%). The transforming DNA was found to be integrated at several different locations, often in multiple tandem copies in the T. reesei genome. In addition, the Escherichia coli β-galactosidase was expressed in T. reesei in enzymatically active form from the A. nidulans gpd promoter.

Original language	English
Pages (from-to)	155-164
Journal	Gene
Volume	61
Issue number	2
DOIs	https://doi.org/10.1016/0378-1119(87)90110-7
Publication status	Published - 1 Jan 1987
MoE publication type	A1 Journal article-refereed

Keywords

cotransformation
heterologous expression
integration
lignocellulose
Recombinant DNA

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10.1016/0378-1119(87)90110-7

Cite this

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title = "A versatile transformation system for the cellulolytic filamentous fungus Trichoderma reesei",

abstract = "An efficient transformation system for the cellulolytic filamentous fungus Trichoderma reesei has been developed. Transformation was obtained with plasmid carrying the dominant selectable marker amdS or the argB gene of Aspergillus nidulans, which was found to complement the respective argB mutation of T. reesei. The transformation frequency can be up to 600 transformants per μg of transforming DNA. The efficiency of co-transformation with unselected DNA was high (approx. 80%). The transforming DNA was found to be integrated at several different locations, often in multiple tandem copies in the T. reesei genome. In addition, the Escherichia coli β-galactosidase was expressed in T. reesei in enzymatically active form from the A. nidulans gpd promoter.",

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T1 - A versatile transformation system for the cellulolytic filamentous fungus Trichoderma reesei

AU - Penttilä, Merja

AU - Nevalainen, Helena

AU - Rättö, Marjaana

AU - Salminen, Elina

AU - Knowles, Jonathan

PY - 1987/1/1

Y1 - 1987/1/1

N2 - An efficient transformation system for the cellulolytic filamentous fungus Trichoderma reesei has been developed. Transformation was obtained with plasmid carrying the dominant selectable marker amdS or the argB gene of Aspergillus nidulans, which was found to complement the respective argB mutation of T. reesei. The transformation frequency can be up to 600 transformants per μg of transforming DNA. The efficiency of co-transformation with unselected DNA was high (approx. 80%). The transforming DNA was found to be integrated at several different locations, often in multiple tandem copies in the T. reesei genome. In addition, the Escherichia coli β-galactosidase was expressed in T. reesei in enzymatically active form from the A. nidulans gpd promoter.

AB - An efficient transformation system for the cellulolytic filamentous fungus Trichoderma reesei has been developed. Transformation was obtained with plasmid carrying the dominant selectable marker amdS or the argB gene of Aspergillus nidulans, which was found to complement the respective argB mutation of T. reesei. The transformation frequency can be up to 600 transformants per μg of transforming DNA. The efficiency of co-transformation with unselected DNA was high (approx. 80%). The transforming DNA was found to be integrated at several different locations, often in multiple tandem copies in the T. reesei genome. In addition, the Escherichia coli β-galactosidase was expressed in T. reesei in enzymatically active form from the A. nidulans gpd promoter.

KW - cotransformation

KW - heterologous expression

KW - integration

KW - lignocellulose

KW - Recombinant DNA

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A versatile transformation system for the cellulolytic filamentous fungus Trichoderma reesei

Abstract

Keywords

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