A xylose-oxidizing membrane-bound aldose dehydrogenase of Gluconobacter oxydans ATCC 621

Johanna Buchert

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

A xylose-oxidizing membrane-bound aldose dehydrogenase was purified from Gluconobacter oxydans subsp suboxydans ATCC 621. The enzyme did not require added coenzyme for activity and could oxidize d-glucose, d-xylose, d-galactose, d-mannose and l-arabinose. d-Glucose was oxidized 1.6 times faster than d-xylose. The enzyme had a molecular mass of 87 kDa and appeared as a single polypeptide. Nitrotetrazolium blue — phenazine methosulphate and dichlorophenol indophenol — phenazine methosulphate were efficient electron acceptors.

Original languageEnglish
Pages (from-to)103 - 114
Number of pages12
JournalJournal of Biotechnology
Volume18
Issue number1
DOIs
Publication statusPublished - 1991
MoE publication typeA1 Journal article-refereed

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