A yeast t-SNARE involved in endocytosis

Karin Seron, Ville Tieaho, Cristina Prescianotto-Baschong, Thomas Aust, Marie-Odile Blondel, Philippe Guillaud, Ginette Devilliers, Olivia Rossanese, Benjamin Glick, Howard Riezman, Sirkka Keränen, Rosine Haguenauer-Tsapis (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

77 Citations (Scopus)

Abstract

The ORF YOL018c (TLG2) of Saccharomyces cerevisiae encodes a protein that belongs to the syntaxin protein family. The proteins of this family, t-SNAREs, are present on target organelles and are thought to participate in the specific interaction between vesicles and acceptor membranes in intracellular membrane trafficking. TLG2 is not an essential gene, and its deletion does not cause defects in the secretory pathway. However, its deletion in cells lacking the vacuolar ATPase subunit Vma2p leads to loss of viability, suggesting that Tlg2p is involved in endocytosis. In tlg2Δ cells, internalization was normal for two endocytic markers, the pheromone α-factor and the plasma membrane uracil permease. In contrast, degradation of α-factor and uracil permease was delayed intlg2Δ cells. Internalization of positively charged Nanogold shows that the endocytic pathway is perturbed in the mutant, which accumulates Nanogold in primary endocytic vesicles and shows a greatly reduced complement of early endosomes. These results strongly suggest that Tlg2p is a t-SNARE involved in early endosome biogenesis.
Original languageEnglish
Pages (from-to)2873-2889
JournalMolecular Biology of the Cell
Volume9
Issue number10
DOIs
Publication statusPublished - 1998
MoE publication typeA1 Journal article-refereed

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    Seron, K., Tieaho, V., Prescianotto-Baschong, C., Aust, T., Blondel, M-O., Guillaud, P., Devilliers, G., Rossanese, O., Glick, B., Riezman, H., Keränen, S., & Haguenauer-Tsapis, R. (1998). A yeast t-SNARE involved in endocytosis. Molecular Biology of the Cell, 9(10), 2873-2889. https://doi.org/10.1091/mbc.9.10.2873