A yeast t-SNARE involved in endocytosis

Karin Seron, Ville Tieaho, Cristina Prescianotto-Baschong, Thomas Aust, Marie-Odile Blondel, Philippe Guillaud, Ginette Devilliers, Olivia Rossanese, Benjamin Glick, Howard Riezman, Sirkka Keränen, Rosine Haguenauer-Tsapis (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

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Abstract

The ORF YOL018c (TLG2) of Saccharomyces cerevisiae encodes a protein that belongs to the syntaxin protein family.
The proteins of this family, t-SNAREs, are present on target organelles and are thought to participate in the specific interaction between vesicles and acceptor membranes in intracellular membrane trafficking. TLG2 is not an essential gene, and its deletion does not cause defects in the secretory pathway. However, its deletion in cells lacking the vacuolar ATPase subunit Vma2p leads to loss of viability, suggesting that Tlg2p is involved in endocytosis.
In tlg2Δ cells, internalization was normal for two endocytic markers, the pheromone α-factor and the plasma membrane uracil permease. In contrast, degradation of α-factor and uracil permease was delayed intlg2Δ cells. Internalization of positively charged Nanogold shows that the endocytic pathway is perturbed in the mutant, which accumulates Nanogold in primary endocytic vesicles and shows a greatly reduced complement of early endosomes.
These results strongly suggest that Tlg2p is a t-SNARE involved in early endosome biogenesis.
Original languageEnglish
Pages (from-to)2873-2889
JournalMolecular Biology of the Cell
Volume9
Issue number10
DOIs
Publication statusPublished - 1998
MoE publication typeA1 Journal article-refereed

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SNARE Proteins
Endocytosis
Membrane Transport Proteins
Uracil
Yeasts
Endosomes
Qa-SNARE Proteins
Vacuolar Proton-Translocating ATPases
Transport Vesicles
Intracellular Membranes
Secretory Pathway
Pheromones
Essential Genes
Gene Deletion
Organelles
Open Reading Frames
Saccharomyces cerevisiae
Proteins
Cell Membrane
Membranes

Cite this

Seron, K., Tieaho, V., Prescianotto-Baschong, C., Aust, T., Blondel, M-O., Guillaud, P., ... Haguenauer-Tsapis, R. (1998). A yeast t-SNARE involved in endocytosis. Molecular Biology of the Cell, 9(10), 2873-2889. https://doi.org/10.1091/mbc.9.10.2873
Seron, Karin ; Tieaho, Ville ; Prescianotto-Baschong, Cristina ; Aust, Thomas ; Blondel, Marie-Odile ; Guillaud, Philippe ; Devilliers, Ginette ; Rossanese, Olivia ; Glick, Benjamin ; Riezman, Howard ; Keränen, Sirkka ; Haguenauer-Tsapis, Rosine. / A yeast t-SNARE involved in endocytosis. In: Molecular Biology of the Cell. 1998 ; Vol. 9, No. 10. pp. 2873-2889.
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title = "A yeast t-SNARE involved in endocytosis",
abstract = "The ORF YOL018c (TLG2) of Saccharomyces cerevisiae encodes a protein that belongs to the syntaxin protein family. The proteins of this family, t-SNAREs, are present on target organelles and are thought to participate in the specific interaction between vesicles and acceptor membranes in intracellular membrane trafficking. TLG2 is not an essential gene, and its deletion does not cause defects in the secretory pathway. However, its deletion in cells lacking the vacuolar ATPase subunit Vma2p leads to loss of viability, suggesting that Tlg2p is involved in endocytosis. In tlg2Δ cells, internalization was normal for two endocytic markers, the pheromone α-factor and the plasma membrane uracil permease. In contrast, degradation of α-factor and uracil permease was delayed intlg2Δ cells. Internalization of positively charged Nanogold shows that the endocytic pathway is perturbed in the mutant, which accumulates Nanogold in primary endocytic vesicles and shows a greatly reduced complement of early endosomes. These results strongly suggest that Tlg2p is a t-SNARE involved in early endosome biogenesis.",
author = "Karin Seron and Ville Tieaho and Cristina Prescianotto-Baschong and Thomas Aust and Marie-Odile Blondel and Philippe Guillaud and Ginette Devilliers and Olivia Rossanese and Benjamin Glick and Howard Riezman and Sirkka Ker{\"a}nen and Rosine Haguenauer-Tsapis",
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language = "English",
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Seron, K, Tieaho, V, Prescianotto-Baschong, C, Aust, T, Blondel, M-O, Guillaud, P, Devilliers, G, Rossanese, O, Glick, B, Riezman, H, Keränen, S & Haguenauer-Tsapis, R 1998, 'A yeast t-SNARE involved in endocytosis', Molecular Biology of the Cell, vol. 9, no. 10, pp. 2873-2889. https://doi.org/10.1091/mbc.9.10.2873

A yeast t-SNARE involved in endocytosis. / Seron, Karin; Tieaho, Ville; Prescianotto-Baschong, Cristina; Aust, Thomas; Blondel, Marie-Odile; Guillaud, Philippe; Devilliers, Ginette; Rossanese, Olivia; Glick, Benjamin; Riezman, Howard; Keränen, Sirkka; Haguenauer-Tsapis, Rosine (Corresponding Author).

In: Molecular Biology of the Cell, Vol. 9, No. 10, 1998, p. 2873-2889.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - A yeast t-SNARE involved in endocytosis

AU - Seron, Karin

AU - Tieaho, Ville

AU - Prescianotto-Baschong, Cristina

AU - Aust, Thomas

AU - Blondel, Marie-Odile

AU - Guillaud, Philippe

AU - Devilliers, Ginette

AU - Rossanese, Olivia

AU - Glick, Benjamin

AU - Riezman, Howard

AU - Keränen, Sirkka

AU - Haguenauer-Tsapis, Rosine

PY - 1998

Y1 - 1998

N2 - The ORF YOL018c (TLG2) of Saccharomyces cerevisiae encodes a protein that belongs to the syntaxin protein family. The proteins of this family, t-SNAREs, are present on target organelles and are thought to participate in the specific interaction between vesicles and acceptor membranes in intracellular membrane trafficking. TLG2 is not an essential gene, and its deletion does not cause defects in the secretory pathway. However, its deletion in cells lacking the vacuolar ATPase subunit Vma2p leads to loss of viability, suggesting that Tlg2p is involved in endocytosis. In tlg2Δ cells, internalization was normal for two endocytic markers, the pheromone α-factor and the plasma membrane uracil permease. In contrast, degradation of α-factor and uracil permease was delayed intlg2Δ cells. Internalization of positively charged Nanogold shows that the endocytic pathway is perturbed in the mutant, which accumulates Nanogold in primary endocytic vesicles and shows a greatly reduced complement of early endosomes. These results strongly suggest that Tlg2p is a t-SNARE involved in early endosome biogenesis.

AB - The ORF YOL018c (TLG2) of Saccharomyces cerevisiae encodes a protein that belongs to the syntaxin protein family. The proteins of this family, t-SNAREs, are present on target organelles and are thought to participate in the specific interaction between vesicles and acceptor membranes in intracellular membrane trafficking. TLG2 is not an essential gene, and its deletion does not cause defects in the secretory pathway. However, its deletion in cells lacking the vacuolar ATPase subunit Vma2p leads to loss of viability, suggesting that Tlg2p is involved in endocytosis. In tlg2Δ cells, internalization was normal for two endocytic markers, the pheromone α-factor and the plasma membrane uracil permease. In contrast, degradation of α-factor and uracil permease was delayed intlg2Δ cells. Internalization of positively charged Nanogold shows that the endocytic pathway is perturbed in the mutant, which accumulates Nanogold in primary endocytic vesicles and shows a greatly reduced complement of early endosomes. These results strongly suggest that Tlg2p is a t-SNARE involved in early endosome biogenesis.

U2 - 10.1091/mbc.9.10.2873

DO - 10.1091/mbc.9.10.2873

M3 - Article

VL - 9

SP - 2873

EP - 2889

JO - Molecular Biology of the Cell

JF - Molecular Biology of the Cell

SN - 1059-1524

IS - 10

ER -

Seron K, Tieaho V, Prescianotto-Baschong C, Aust T, Blondel M-O, Guillaud P et al. A yeast t-SNARE involved in endocytosis. Molecular Biology of the Cell. 1998;9(10):2873-2889. https://doi.org/10.1091/mbc.9.10.2873