A ZO-1/a5ß1-integrin complex regulates cytokinesis downstream of PKCe in NCI-H460 cells plated on fibronectin

Saara Hämälistö, Jeroen Pouwels, Nicola De Franceschi, Markku Saari, Ylva Ivarsson, Pascale Zimmermann, Andreas Brech, Harald Stenmark, Johanna Ivaska (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

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Abstract

Recently, we demonstrated that integrin adhesion to the extracellular matrix at the cleavage furrow is essential for cytokinesis of adherent cells. Here, we report that tight junction protein ZO-1 (Zonula Occludens-1) is required for successful cytokinesis in NCI-H460 cells plated on fibronectin. This function of ZO-1 involves interaction with the cytoplasmic domain of α5-integrin to facilitate recruitment of active fibronectin-binding integrins to the base of the cleavage furrow. In the absence of ZO-1, or a functional ZO-1/α5β1-integrin complex, proper actin-dependent constriction between daughter cells is impaired and cells fail cytokinesis. Super-resolution microscopy reveals that in ZO-1 depleted cells the furrow becomes delocalized from the matrix. We also show that PKCε-dependent phosphorylation at Serine168 is required for ZO-1 localization to the furrow and successful cell division. Altogether, our results identify a novel regulatory pathway involving the interplay between ZO-1, α5-integrin and PKCε in the late stages of mammalian cell division.
Original languageEnglish
Article number70696
JournalPLoS ONE
Volume8
Issue number8
DOIs
Publication statusPublished - 2013
MoE publication typeA1 Journal article-refereed

Fingerprint

Cytokinesis
tight junctions
Tight Junctions
fibronectins
cytokinesis
integrins
Fibronectins
Integrins
furrows
cells
Cells
Cell Division
Tight Junction Proteins
Phosphorylation
cell division
Actins
Microscopic examination
Adhesion
Constriction
Extracellular Matrix

Cite this

Hämälistö, S., Pouwels, J., De Franceschi, N., Saari, M., Ivarsson, Y., Zimmermann, P., ... Ivaska, J. (2013). A ZO-1/a5ß1-integrin complex regulates cytokinesis downstream of PKCe in NCI-H460 cells plated on fibronectin. PLoS ONE, 8(8), [70696]. https://doi.org/10.1371/journal.pone.0070696
Hämälistö, Saara ; Pouwels, Jeroen ; De Franceschi, Nicola ; Saari, Markku ; Ivarsson, Ylva ; Zimmermann, Pascale ; Brech, Andreas ; Stenmark, Harald ; Ivaska, Johanna. / A ZO-1/a5ß1-integrin complex regulates cytokinesis downstream of PKCe in NCI-H460 cells plated on fibronectin. In: PLoS ONE. 2013 ; Vol. 8, No. 8.
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abstract = "Recently, we demonstrated that integrin adhesion to the extracellular matrix at the cleavage furrow is essential for cytokinesis of adherent cells. Here, we report that tight junction protein ZO-1 (Zonula Occludens-1) is required for successful cytokinesis in NCI-H460 cells plated on fibronectin. This function of ZO-1 involves interaction with the cytoplasmic domain of α5-integrin to facilitate recruitment of active fibronectin-binding integrins to the base of the cleavage furrow. In the absence of ZO-1, or a functional ZO-1/α5β1-integrin complex, proper actin-dependent constriction between daughter cells is impaired and cells fail cytokinesis. Super-resolution microscopy reveals that in ZO-1 depleted cells the furrow becomes delocalized from the matrix. We also show that PKCε-dependent phosphorylation at Serine168 is required for ZO-1 localization to the furrow and successful cell division. Altogether, our results identify a novel regulatory pathway involving the interplay between ZO-1, α5-integrin and PKCε in the late stages of mammalian cell division.",
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Hämälistö, S, Pouwels, J, De Franceschi, N, Saari, M, Ivarsson, Y, Zimmermann, P, Brech, A, Stenmark, H & Ivaska, J 2013, 'A ZO-1/a5ß1-integrin complex regulates cytokinesis downstream of PKCe in NCI-H460 cells plated on fibronectin', PLoS ONE, vol. 8, no. 8, 70696. https://doi.org/10.1371/journal.pone.0070696

A ZO-1/a5ß1-integrin complex regulates cytokinesis downstream of PKCe in NCI-H460 cells plated on fibronectin. / Hämälistö, Saara; Pouwels, Jeroen; De Franceschi, Nicola; Saari, Markku; Ivarsson, Ylva; Zimmermann, Pascale; Brech, Andreas; Stenmark, Harald; Ivaska, Johanna (Corresponding Author).

In: PLoS ONE, Vol. 8, No. 8, 70696, 2013.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - A ZO-1/a5ß1-integrin complex regulates cytokinesis downstream of PKCe in NCI-H460 cells plated on fibronectin

AU - Hämälistö, Saara

AU - Pouwels, Jeroen

AU - De Franceschi, Nicola

AU - Saari, Markku

AU - Ivarsson, Ylva

AU - Zimmermann, Pascale

AU - Brech, Andreas

AU - Stenmark, Harald

AU - Ivaska, Johanna

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N2 - Recently, we demonstrated that integrin adhesion to the extracellular matrix at the cleavage furrow is essential for cytokinesis of adherent cells. Here, we report that tight junction protein ZO-1 (Zonula Occludens-1) is required for successful cytokinesis in NCI-H460 cells plated on fibronectin. This function of ZO-1 involves interaction with the cytoplasmic domain of α5-integrin to facilitate recruitment of active fibronectin-binding integrins to the base of the cleavage furrow. In the absence of ZO-1, or a functional ZO-1/α5β1-integrin complex, proper actin-dependent constriction between daughter cells is impaired and cells fail cytokinesis. Super-resolution microscopy reveals that in ZO-1 depleted cells the furrow becomes delocalized from the matrix. We also show that PKCε-dependent phosphorylation at Serine168 is required for ZO-1 localization to the furrow and successful cell division. Altogether, our results identify a novel regulatory pathway involving the interplay between ZO-1, α5-integrin and PKCε in the late stages of mammalian cell division.

AB - Recently, we demonstrated that integrin adhesion to the extracellular matrix at the cleavage furrow is essential for cytokinesis of adherent cells. Here, we report that tight junction protein ZO-1 (Zonula Occludens-1) is required for successful cytokinesis in NCI-H460 cells plated on fibronectin. This function of ZO-1 involves interaction with the cytoplasmic domain of α5-integrin to facilitate recruitment of active fibronectin-binding integrins to the base of the cleavage furrow. In the absence of ZO-1, or a functional ZO-1/α5β1-integrin complex, proper actin-dependent constriction between daughter cells is impaired and cells fail cytokinesis. Super-resolution microscopy reveals that in ZO-1 depleted cells the furrow becomes delocalized from the matrix. We also show that PKCε-dependent phosphorylation at Serine168 is required for ZO-1 localization to the furrow and successful cell division. Altogether, our results identify a novel regulatory pathway involving the interplay between ZO-1, α5-integrin and PKCε in the late stages of mammalian cell division.

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