ACEI is a repressor of cellulase and xylanase genes in Trichoderma reesei

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsScientific

Abstract

T. reesei is an efficient producer of cellulolytic and xylanolytic enzymes. In general, cellulose and its derivatives and different xylans induce cellulase and xylanase expression while glucose represses cellulase and xylanase genes. The production of these enzymes is regulated at the transcriptional level at least by the glucose repressor CREI and the activator ACEII. We have isolated a third factor, ACEI, that binds to and activates the main cellulase promoter cbh1in vivo in yeast. ACEI is a Cys2-His2 type of a transcription factor that binds in vitro to eight sites scattered along the cbh1 promoter containing the core 5'AGGCA sequence. Although originally isolated as a protein capable of activating thecbh1 promoter of T. reesei in S. cerevisiae, further studies oface1 deletion strain indicated that ACEI is a repressor of cellulase and xylanase genes. The deletion of the ace1 gene led up to 10 times higher expression of cellulase genes, cbh1, cbh2, egl1, and egl2 after the transfer of glycerol grown mycelia to cellulose media or when the cellulase genes were induced by the addition of a disaccharide sophorose into glycerol media. Similarly, the expression of xyn1 and xyn2 was increased by ace1 deletion. Deletion of the ace2 gene, encoding the ACEII activator, in a strain deleted for ace1 did not affect the high level of cellulase expression seen in the ace1 deletion strain indicating, that there is at least one additional cellulase and xylanase activator present in T. reesei.
Original languageEnglish
Title of host publication22nd Fungal Genetics Conference at Asilomar
Subtitle of host publicationAbstracts from the 2003 Fungal Genetics Conference at Asilomar
DOIs
Publication statusPublished - 2003
Event22nd Fungal Genetics Conference - Asilomar, United States
Duration: 18 Mar 200322 Mar 2003

Publication series

NameFungal Genetics Reports
PublisherNew Prairie Press
NumberArticle 18
Volume50

Conference

Conference22nd Fungal Genetics Conference
CountryUnited States
CityAsilomar
Period18/03/0322/03/03

Fingerprint

Trichoderma reesei
xylanases
endo-1,4-beta-glucanase
genes
gene deletion
promoter regions
glycerol
cellulose
glucose
disaccharides
xylan
enzymes
mycelium
transcription factors
yeasts

Cite this

Aro, N., Ilmen, M., Saloheimo, A., & Penttilä, M. (2003). ACEI is a repressor of cellulase and xylanase genes in Trichoderma reesei. In 22nd Fungal Genetics Conference at Asilomar: Abstracts from the 2003 Fungal Genetics Conference at Asilomar [212] Fungal Genetics Reports, No. Article 18, Vol.. 50 https://doi.org/10.4148/1941-4765.1164
Aro, Nina ; Ilmen, Marja ; Saloheimo, Anu ; Penttilä, Merja. / ACEI is a repressor of cellulase and xylanase genes in Trichoderma reesei. 22nd Fungal Genetics Conference at Asilomar: Abstracts from the 2003 Fungal Genetics Conference at Asilomar. 2003. (Fungal Genetics Reports; No. Article 18, Vol. 50).
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abstract = "T. reesei is an efficient producer of cellulolytic and xylanolytic enzymes. In general, cellulose and its derivatives and different xylans induce cellulase and xylanase expression while glucose represses cellulase and xylanase genes. The production of these enzymes is regulated at the transcriptional level at least by the glucose repressor CREI and the activator ACEII. We have isolated a third factor, ACEI, that binds to and activates the main cellulase promoter cbh1in vivo in yeast. ACEI is a Cys2-His2 type of a transcription factor that binds in vitro to eight sites scattered along the cbh1 promoter containing the core 5'AGGCA sequence. Although originally isolated as a protein capable of activating thecbh1 promoter of T. reesei in S. cerevisiae, further studies oface1 deletion strain indicated that ACEI is a repressor of cellulase and xylanase genes. The deletion of the ace1 gene led up to 10 times higher expression of cellulase genes, cbh1, cbh2, egl1, and egl2 after the transfer of glycerol grown mycelia to cellulose media or when the cellulase genes were induced by the addition of a disaccharide sophorose into glycerol media. Similarly, the expression of xyn1 and xyn2 was increased by ace1 deletion. Deletion of the ace2 gene, encoding the ACEII activator, in a strain deleted for ace1 did not affect the high level of cellulase expression seen in the ace1 deletion strain indicating, that there is at least one additional cellulase and xylanase activator present in T. reesei.",
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Aro, N, Ilmen, M, Saloheimo, A & Penttilä, M 2003, ACEI is a repressor of cellulase and xylanase genes in Trichoderma reesei. in 22nd Fungal Genetics Conference at Asilomar: Abstracts from the 2003 Fungal Genetics Conference at Asilomar., 212, Fungal Genetics Reports, no. Article 18, vol. 50, 22nd Fungal Genetics Conference, Asilomar, United States, 18/03/03. https://doi.org/10.4148/1941-4765.1164

ACEI is a repressor of cellulase and xylanase genes in Trichoderma reesei. / Aro, Nina; Ilmen, Marja; Saloheimo, Anu; Penttilä, Merja.

22nd Fungal Genetics Conference at Asilomar: Abstracts from the 2003 Fungal Genetics Conference at Asilomar. 2003. 212 (Fungal Genetics Reports; No. Article 18, Vol. 50).

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsScientific

TY - CHAP

T1 - ACEI is a repressor of cellulase and xylanase genes in Trichoderma reesei

AU - Aro, Nina

AU - Ilmen, Marja

AU - Saloheimo, Anu

AU - Penttilä, Merja

PY - 2003

Y1 - 2003

N2 - T. reesei is an efficient producer of cellulolytic and xylanolytic enzymes. In general, cellulose and its derivatives and different xylans induce cellulase and xylanase expression while glucose represses cellulase and xylanase genes. The production of these enzymes is regulated at the transcriptional level at least by the glucose repressor CREI and the activator ACEII. We have isolated a third factor, ACEI, that binds to and activates the main cellulase promoter cbh1in vivo in yeast. ACEI is a Cys2-His2 type of a transcription factor that binds in vitro to eight sites scattered along the cbh1 promoter containing the core 5'AGGCA sequence. Although originally isolated as a protein capable of activating thecbh1 promoter of T. reesei in S. cerevisiae, further studies oface1 deletion strain indicated that ACEI is a repressor of cellulase and xylanase genes. The deletion of the ace1 gene led up to 10 times higher expression of cellulase genes, cbh1, cbh2, egl1, and egl2 after the transfer of glycerol grown mycelia to cellulose media or when the cellulase genes were induced by the addition of a disaccharide sophorose into glycerol media. Similarly, the expression of xyn1 and xyn2 was increased by ace1 deletion. Deletion of the ace2 gene, encoding the ACEII activator, in a strain deleted for ace1 did not affect the high level of cellulase expression seen in the ace1 deletion strain indicating, that there is at least one additional cellulase and xylanase activator present in T. reesei.

AB - T. reesei is an efficient producer of cellulolytic and xylanolytic enzymes. In general, cellulose and its derivatives and different xylans induce cellulase and xylanase expression while glucose represses cellulase and xylanase genes. The production of these enzymes is regulated at the transcriptional level at least by the glucose repressor CREI and the activator ACEII. We have isolated a third factor, ACEI, that binds to and activates the main cellulase promoter cbh1in vivo in yeast. ACEI is a Cys2-His2 type of a transcription factor that binds in vitro to eight sites scattered along the cbh1 promoter containing the core 5'AGGCA sequence. Although originally isolated as a protein capable of activating thecbh1 promoter of T. reesei in S. cerevisiae, further studies oface1 deletion strain indicated that ACEI is a repressor of cellulase and xylanase genes. The deletion of the ace1 gene led up to 10 times higher expression of cellulase genes, cbh1, cbh2, egl1, and egl2 after the transfer of glycerol grown mycelia to cellulose media or when the cellulase genes were induced by the addition of a disaccharide sophorose into glycerol media. Similarly, the expression of xyn1 and xyn2 was increased by ace1 deletion. Deletion of the ace2 gene, encoding the ACEII activator, in a strain deleted for ace1 did not affect the high level of cellulase expression seen in the ace1 deletion strain indicating, that there is at least one additional cellulase and xylanase activator present in T. reesei.

UR - http://newprairiepress.org/cgi/viewcontent.cgi?article=1164&context=fgr

U2 - 10.4148/1941-4765.1164

DO - 10.4148/1941-4765.1164

M3 - Conference abstract in proceedings

T3 - Fungal Genetics Reports

BT - 22nd Fungal Genetics Conference at Asilomar

ER -

Aro N, Ilmen M, Saloheimo A, Penttilä M. ACEI is a repressor of cellulase and xylanase genes in Trichoderma reesei. In 22nd Fungal Genetics Conference at Asilomar: Abstracts from the 2003 Fungal Genetics Conference at Asilomar. 2003. 212. (Fungal Genetics Reports; No. Article 18, Vol. 50). https://doi.org/10.4148/1941-4765.1164