Acetylcholinesterase inhibitory guided fractionation of Melissa officinalis L.

Keyvan Dastmalchi, Velimatti Ollilainen, Petri Lackman, Gustav Boije af Gennäs, H.J. Damien Dorman (Corresponding Author), Päivi P. Järvinen, Jari Yli-Kauhaluoma, Raimo Hiltunen

Research output: Contribution to journalArticleScientificpeer-review

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Abstract

The plant Melissa officinalis L. has been used traditionally in the treatment of cognitive dysfunction. Based on its traditional medicinal use, it was assessed for its clinical efficacy in mild to moderate Alzheimer’s patients. The plant was effective in the management of the disease. Therefore, based on this result, a similar plant extract was prepared in order to be screened for bioactivities which are relevant in Alzheimer’s disease therapy. The extract was recently screened for antioxidant activity and it showed a wide range of antioxidant properties. Another important bioactivity is acetylcholinesterase inhibition, which the extract was screened for in the current investigation. The extract was capable of inhibiting the enzyme in a time and dose-dependent manner. Activity of the extract at 10 min was estimated as 1.72 ± 0.16 μg equivalents of physostigmine/mg of the extract. Acetylcholinesterase inhibitory guided fractionation of the extract was then carried out. Most of the fractions showed inhibitory activity and were more potent than the extract. The contents of the most potent fraction were identified as cis- and trans-rosmarinic acid isomers and a rosmarinic acid derivative using LC-DAD-ESI-MS and NMR methods.
Original languageEnglish
Pages (from-to)867-871
JournalBioorganic & Medicinal Chemistry
Volume17
Issue number2
DOIs
Publication statusPublished - 2009
MoE publication typeA1 Journal article-refereed

Fingerprint

Melissa
Acetylcholinesterase
Fractionation
Bioactivity
Antioxidants
Physostigmine
Plant Extracts
Disease Management
Isomers
Alzheimer Disease
Nuclear magnetic resonance
Derivatives
Enzymes
Therapeutics
rosmarinic acid

Keywords

  • Melissa officinalis
  • activity-guided fractionation
  • acetylcholinesterase inhibition
  • cis-rosmarinic acid
  • trans-rosmarinic acid

Cite this

Dastmalchi, K., Ollilainen, V., Lackman, P., Boije af Gennäs, G., Dorman, H. J. D., Järvinen, P. P., ... Hiltunen, R. (2009). Acetylcholinesterase inhibitory guided fractionation of Melissa officinalis L. Bioorganic & Medicinal Chemistry, 17(2), 867-871. https://doi.org/10.1016/j.bmc.2008.11.034
Dastmalchi, Keyvan ; Ollilainen, Velimatti ; Lackman, Petri ; Boije af Gennäs, Gustav ; Dorman, H.J. Damien ; Järvinen, Päivi P. ; Yli-Kauhaluoma, Jari ; Hiltunen, Raimo. / Acetylcholinesterase inhibitory guided fractionation of Melissa officinalis L. In: Bioorganic & Medicinal Chemistry. 2009 ; Vol. 17, No. 2. pp. 867-871.
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abstract = "The plant Melissa officinalis L. has been used traditionally in the treatment of cognitive dysfunction. Based on its traditional medicinal use, it was assessed for its clinical efficacy in mild to moderate Alzheimer’s patients. The plant was effective in the management of the disease. Therefore, based on this result, a similar plant extract was prepared in order to be screened for bioactivities which are relevant in Alzheimer’s disease therapy. The extract was recently screened for antioxidant activity and it showed a wide range of antioxidant properties. Another important bioactivity is acetylcholinesterase inhibition, which the extract was screened for in the current investigation. The extract was capable of inhibiting the enzyme in a time and dose-dependent manner. Activity of the extract at 10 min was estimated as 1.72 ± 0.16 μg equivalents of physostigmine/mg of the extract. Acetylcholinesterase inhibitory guided fractionation of the extract was then carried out. Most of the fractions showed inhibitory activity and were more potent than the extract. The contents of the most potent fraction were identified as cis- and trans-rosmarinic acid isomers and a rosmarinic acid derivative using LC-DAD-ESI-MS and NMR methods.",
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Dastmalchi, K, Ollilainen, V, Lackman, P, Boije af Gennäs, G, Dorman, HJD, Järvinen, PP, Yli-Kauhaluoma, J & Hiltunen, R 2009, 'Acetylcholinesterase inhibitory guided fractionation of Melissa officinalis L.', Bioorganic & Medicinal Chemistry, vol. 17, no. 2, pp. 867-871. https://doi.org/10.1016/j.bmc.2008.11.034

Acetylcholinesterase inhibitory guided fractionation of Melissa officinalis L.. / Dastmalchi, Keyvan; Ollilainen, Velimatti; Lackman, Petri; Boije af Gennäs, Gustav; Dorman, H.J. Damien (Corresponding Author); Järvinen, Päivi P.; Yli-Kauhaluoma, Jari; Hiltunen, Raimo.

In: Bioorganic & Medicinal Chemistry, Vol. 17, No. 2, 2009, p. 867-871.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Acetylcholinesterase inhibitory guided fractionation of Melissa officinalis L.

AU - Dastmalchi, Keyvan

AU - Ollilainen, Velimatti

AU - Lackman, Petri

AU - Boije af Gennäs, Gustav

AU - Dorman, H.J. Damien

AU - Järvinen, Päivi P.

AU - Yli-Kauhaluoma, Jari

AU - Hiltunen, Raimo

PY - 2009

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N2 - The plant Melissa officinalis L. has been used traditionally in the treatment of cognitive dysfunction. Based on its traditional medicinal use, it was assessed for its clinical efficacy in mild to moderate Alzheimer’s patients. The plant was effective in the management of the disease. Therefore, based on this result, a similar plant extract was prepared in order to be screened for bioactivities which are relevant in Alzheimer’s disease therapy. The extract was recently screened for antioxidant activity and it showed a wide range of antioxidant properties. Another important bioactivity is acetylcholinesterase inhibition, which the extract was screened for in the current investigation. The extract was capable of inhibiting the enzyme in a time and dose-dependent manner. Activity of the extract at 10 min was estimated as 1.72 ± 0.16 μg equivalents of physostigmine/mg of the extract. Acetylcholinesterase inhibitory guided fractionation of the extract was then carried out. Most of the fractions showed inhibitory activity and were more potent than the extract. The contents of the most potent fraction were identified as cis- and trans-rosmarinic acid isomers and a rosmarinic acid derivative using LC-DAD-ESI-MS and NMR methods.

AB - The plant Melissa officinalis L. has been used traditionally in the treatment of cognitive dysfunction. Based on its traditional medicinal use, it was assessed for its clinical efficacy in mild to moderate Alzheimer’s patients. The plant was effective in the management of the disease. Therefore, based on this result, a similar plant extract was prepared in order to be screened for bioactivities which are relevant in Alzheimer’s disease therapy. The extract was recently screened for antioxidant activity and it showed a wide range of antioxidant properties. Another important bioactivity is acetylcholinesterase inhibition, which the extract was screened for in the current investigation. The extract was capable of inhibiting the enzyme in a time and dose-dependent manner. Activity of the extract at 10 min was estimated as 1.72 ± 0.16 μg equivalents of physostigmine/mg of the extract. Acetylcholinesterase inhibitory guided fractionation of the extract was then carried out. Most of the fractions showed inhibitory activity and were more potent than the extract. The contents of the most potent fraction were identified as cis- and trans-rosmarinic acid isomers and a rosmarinic acid derivative using LC-DAD-ESI-MS and NMR methods.

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KW - activity-guided fractionation

KW - acetylcholinesterase inhibition

KW - cis-rosmarinic acid

KW - trans-rosmarinic acid

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DO - 10.1016/j.bmc.2008.11.034

M3 - Article

VL - 17

SP - 867

EP - 871

JO - Bioorganic & Medicinal Chemistry

JF - Bioorganic & Medicinal Chemistry

SN - 0968-0896

IS - 2

ER -

Dastmalchi K, Ollilainen V, Lackman P, Boije af Gennäs G, Dorman HJD, Järvinen PP et al. Acetylcholinesterase inhibitory guided fractionation of Melissa officinalis L. Bioorganic & Medicinal Chemistry. 2009;17(2):867-871. https://doi.org/10.1016/j.bmc.2008.11.034